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BMC Vet Res. 2012; 8: 71.
Published online May 28, 2012. doi:  10.1186/1746-6148-8-71
PMCID: PMC3489612
Exploring flubendazole formulations for use in sheep. Pharmacokinetic evaluation of a cyclodextrin-based solution
Laura Ceballos,corresponding author1,2 Laura Moreno,1,2 Juan J Torrado,2,3 Carlos Lanusse,1,2 and Luis Alvarez1,2
1Departamento de Fisiopatología, Laboratorio de Farmacología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Campus Universitario, 7000, Tandil, Argentina
2Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Tandil, Argentina
3Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain
corresponding authorCorresponding author.
Laura Ceballos: ceballos/at/vet.unicen.edu.ar; Laura Moreno: lmoreno/at/vet.unicen.edu.ar; Juan J Torrado: torrado1/at/farm.ucm.es; Carlos Lanusse: clanusse/at/vet.unicen.edu.ar; Luis Alvarez: lalvarez/at/vet.unicen.edu.ar
Received November 22, 2011; Accepted April 17, 2012.
Abstract
Background
Flubendazole (FLBZ) is a poor water solubility broad-spectrum BZD methylcarbamate anthelmintic compound. Cyclodextrins (CDs) are usually used to increase aqueous solubility of poor hydrosoluble compounds. The comparative in vitro aqueous solubility of FLBZ and other BZD anthelmintics in the presence of hydroxypropyl-β-cyclodextrin (HPβCD) was evaluated in the current work. Additionally, the comparative pharmacokinetic behaviour of FLBZ (and its metabolites) administered by the intraruminal (i.r.) or intraabomasal (i.a.) routes to sheep as either an aqueous CDs-based solution or a conventional carboximethylcellulose (CMC) suspension was assessed. Drug solubility studies involving albendazole, mebendazole, oxfendazole and FLBZ were performed in an aqueous solution (pH 1.2 or 7.4) with or without HPβCD (10%, w/v). The pharmacokinetic study involved two experiments. Experiment 1: In a crossover study, sheep received either a FLBZ-CDs solution (n = 3) or a FLBZ-CMC suspension (n = 3) by the i.r. route (3.8 mg/kg). The treatment Groups were reversed after a 21-days washout period. Experiment 2: sheep (n = 4) were treated by the i.a. route with the FLBZ-CDs solution (3.8 mg/kg). Plasma and abomasal fluid samples were collected between 0 and 72 h post-treatment. Samples were analysed by HPLC.
Results
Improvement of FLBZ aqueous solubility due to CDs resulted markedly higher than that observed for mebendazole and albendazole. However, oppositely to what was expected, the absorption-related pharmacokinetic parameters did not show any marked formulation-dependant effect. After the i.a. administration of FLBZ, the AUC and the Tmax of the parent compound were significantly (P < 0.05) reduced, which is consistent with ruminal bypass.
Conclusion
The administration of FLBZ as a CDs-based solution, does not seem to achieve great practical relevance for parasite control in sheep.
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