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Logo of bmcvetresBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Veterinary Research
 
BMC Vet Res. 2012; 8: 143.
Published online Aug 25, 2012. doi:  10.1186/1746-6148-8-143
PMCID: PMC3487956
Modulation of ovine SBD-1 expression by 17beta-estradiol in ovine oviduct epithelial cells
Shiyong Wen,1,2 Guifang Cao,corresponding author1,2 Tuya Bao,3 LanLing Cheng,1 Haijun Li,corresponding author1 Chenguang Du,4 Yong Tu,5 Qi Li,1 Ruizhen Jian,1 Pengwei Zhao,6 and Wuriliga7
1College of veterinary, Inner Mongolia Agricultural University, Huhhot, 010018, People’s Republic of China
2Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Hohhot, 010018, People’s Republic of China
3School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, 010059, People’s Republic of China
4Vocational and Technical College, Inner Mongolia Agricultural University, Baotou, 014109, People’s Republic of China
5Keshiketeng Banner Animal Disease Control Center, Chifeng, 025350, People’s Republic of China
6Pathogenic Organisms and Immunology Lab, basic medical college, Inner Mongolia Medical University, Hohhot, 010059, People’s Republic of China
7College of animal science, Inner Mongolia Agricultural University, Huhhot, 010018, People’s Republic of China
corresponding authorCorresponding author.
Shiyong Wen: wenshiyong434/at/163.com; Guifang Cao: guifangcao/at/hotmail.com; Tuya Bao: baotuya/at/126.com; LanLing Cheng: chenglanling1234/at/126.com; Haijun Li: navy1973/at/163.com; Chenguang Du: dcglxj/at/163.com; Yong Tu: xiaofei_12/at/163.com; Qi Li: liqi253762/at/163.com; Ruizhen Jian: jianruizhen0503/at/163.com; Pengwei Zhao: pengwzhao/at/126.com; Wuriliga: wuriliga1987/at/yahoo.cn
Received March 8, 2012; Accepted August 10, 2012.
Abstract
Background
Mucosal epithelia, including those of the oviduct, secrete antimicrobial innate immune molecules (AIIMS). These have bactericidal/bacteriostatic functions against a variety of pathogens. Among the AIIMs, sheep β-defensin-1 (SBD-1) is one of the most potent. Even though the SBD-1 is an important AIIM and it is regulated closely by estrogenic hormone, the regulation mechanism of 17β-estradiol has not been clearly established. We investigated the effects of E2 and agonist or inhibitor on ovine oviduct epithelial cells in regard to SBD-1 expression using reverse transcription quantitative PCR (RT-qPCR). In addition, three different pathways were inhibited separately or simultaneously to confirm the effect of different inhibitors in the regulation mechanism.
Results
17beta-estradiol (E2) induced release of SBD-1 in ovine oviduct epithelial cells. SBD-1 expression was mediated through G-protein-coupled receptor 30 (GPR30) and Estrogen Receptors (ERs) activation in ovine oviduct epithelial cell. Inhibition of gene expression of protein kinase A (PKA), protein kinase C (PKC), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) led to a decreased SBD-1 expression.
Conclusions
Taken together, E2-induced up-regulation of SBD-1 expressions were GPR30-dependent during prophase and ERs-dependent during later-stage in ovine oviduct epithelial cells, and we assume that the effect was completed by the PKA, PKC, and NF-κB pathways simultaneous.
Keywords: Sheep, Oviduct epithelial, SBD-1, Modulation, Signaling pathway
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