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Ocean viruses are ubiquitous and abundant and play important roles in global biogeochemical cycles by means of their mortality, horizontal gene transfer, and manipulation of host metabolism. However, the obstacles involved in linking viruses to their hosts in a high-throughput manner bottlenecks our ability to understand virus-host interactions in complex communities. We have developed a method called viral tagging (VT), which combines mixtures of host cells and fluorescent viruses with flow cytometry. We investigated multiple viruses which infect each of two model marine bacteria that represent the slow-growing, photoautotrophic genus Synechococcus (Cyanobacteria) and the fast-growing, heterotrophic genus Pseudoalteromonas (Gammaproteobacteria). Overall, viral tagging results for viral infection were consistent with plaque and liquid infection assays for cyanobacterial myo-, podo- and siphoviruses and some (myo- and podoviruses) but not all (four siphoviruses) heterotrophic bacterial viruses. Virus-tagged Pseudoalteromonas organisms were proportional to the added viruses under varied infection conditions (virus-bacterium ratios), while no more than 50% of the Synechococcus organisms were virus tagged even at viral abundances that exceeded (5 to 10×) that of their hosts. Further, we found that host growth phase minimally impacts the fraction of virus-tagged Synechococcus organisms while greatly affecting phage adsorption to Pseudoalteromonas. Together these findings suggest that at least two contrasting viral life strategies exist in the oceans and that they likely reflect adaptation to their host microbes. Looking forward to the point at which the virus-tagging signature is well understood (e.g., for Synechococcus), application to natural communities should begin to provide population genomic data at the proper scale for predictively modeling two of the most abundant biological entities on Earth.
Viral study suffers from an inability to link viruses to hosts en masse, and yet delineating “who infects whom” is fundamental to viral ecology and predictive modeling. This article describes viral tagging—a high-throughput method to investigate virus-host interactions by combining the fluorescent labeling of viruses for “tagging” host cells that can be analyzed and sorted using flow cytometry. Two cultivated hosts (the cyanobacterium Synechococcus and the gammaproteobacterium Pseudoalteromonas) and their viruses (podo-, myo-, and siphoviruses) were investigated to validate the method. These lab-based experiments indicate that for most virus-host pairings, VT (viral tagging) adsorption is equivalent to traditional infection by liquid and plaque assays, with the exceptions being confined to promiscuous adsorption by Pseudoalteromonas siphoviruses. These experiments also reveal variability in life strategies across these oceanic virus-host systems with respect to infection conditions and host growth status, which highlights the need for further model system characterization to break open this virus-host interaction “black box.”
The oceans cover more than 70% of the Earth’s surface and are dominated by complex and dynamic microbial communities, both in terms of biomass and metabolism (reviewed in reference 1). Phototrophic microorganisms harvest solar energy and produce the organic matter that fuels nearly all processes in the ocean (2, 3), while their heterotrophic counterparts respire this fixed organic carbon. While global carbon cycling is fundamental to predictive modeling of climate change and represents one of the best-studied elemental cycling processes on Earth, our understanding of the balance between fixed and respired carbon on a global scale remains poorly understood (reviewed in reference 4).
With respect to carbon cycling, viruses have largely been ignored due to lack of data. This is in spite of the fact that, at least in the oceans, viruses are abundant (~106 to 108 ml−1 water), kill ~20 to 40% of bacteria per day, and are responsible for large carbon fluxes in the oceans at 150 Gt year−1 (5). However, their carbon cycling impact is likely much larger. For example, cyanobacterial viruses (cyanophages) also contain photosynthesis genes, including the core photosystem gene psbA and sometimes psbD (6–10), as well as other auxiliary metabolic genes (AMGs ; e.g., pebS and talC) that are expressed during infection (12–15) and are likely critical to ocean cyanophage infection. In fact, psbA is modeled to increase phage fitness (16, 17), and in ocean surface microbial metagenomes, viral versions, rather than microbial versions, dominate the psbA signal—at least where organismal origin can be informatically identified (8). Thus, while no quantitative community proteomic data are yet available, ocean photosynthetic carbon fixation is at least partly mediated by virus-encoded core photosynthesis proteins, thereby furthering the viral impact on global biogeochemistry.
As ocean ecology attempts to advance from observations to predictions, modeling becomes of fundamental importance. While two decades of viral ecology research has focused on community level understanding through measuring viral abundance, production/decay, and frequency of infected cells (for example, see references 18 to 24), little is known about the fundamental issue of “who infects whom.”
While cultivation-based methods directly link a virus strain to its host, it is impossible to characterize millions of viruses per milliliter of seawater and thousands to hundreds of thousands of viral types per sampling site (25) using traditional low-throughput cultivation methods. Fortunately, new methods are on the way. First, microfluidic digital PCR now allows a researcher to document colocalized host and viral gene products, as long as genetic information is available for primer design for both virus and host (26). Second, Escherichia coli O157-specific immunomagnetic beads coated with fluorescently labeled viruses use the virus-host interaction to fluorescently label E. coli for detection using flow cytometry (27, 28), but this method currently requires high cell concentrations (104 cells ml−1) for detection and the availability of immunomagnetic beads specific to individual host strains (27, 28). Third, fluorescently labeled viruses (FLVs) have been used as probes to tag their host cells for examination under the microscope (29–31), a method that is limited only by the fact that it is low throughput. Additionally, while FLVs and flow cytometry have been combined to increase throughput (32), labeling of nonhost cells has plagued these experiments even when a thymidine analog (EdU [5-ethynyl-2′-deoxyuridine]) was used as an alternative virus-labeling agent (32).
Here we expand upon the above-described use of FLVs to tag host cells by (i) optimizing staining, (ii) incorporating flow cytometry to enable high-throughput detection of infected host cells (and sequencing in future work), and (iii) validating the methods by comparison against infection assays via controlled laboratory experiments. We then applied the optimized viral tagging (VT) method to two marine model virus-host systems—the slow-growing (doubling time ~ 24 h), photoautotrophic cyanobacterium Synechococcus and the fast-growing (doubling time ~ 1 h), heterotrophic alphaproteobacterium Pseudoalteromonas and their specific viruses—to investigate how they are affected by various infection and growth conditions. These two model systems make ideal candidates for this study, as they are widespread and ecologically important in the oceans (for example, see references 5, 19, and 33 to 37).
The objective of this study was to couple the use of FLVs for tagging their host cells (29–31) with high-throughput flow cytometry for rapid, culture-independent assessment of host-virus pairings initially in controlled, laboratory studies (this study) for eventual application in natural complex communities (future work). Here we developed and tested VT on multiple viral isolates from each of two well-studied marine virus-host model systems.
To develop a robust method, we first optimized all three steps of the VT process: (i) viral staining, (ii) viral washing, and (iii) examining the mixture of bacteria and viruses using flow cytometry.
To optimize viral staining, we evaluated three stains (SYBR gold, SYBR green II, and SYBR Safe) and three previously described incubation conditions (80°C for 10 min, room temperature for 10 min, and 4°C overnight ) using the cyanophage Syn33 and the host strain Synechococcus WH7803 (see Fig. S1 in the supplemental material). SYBR gold at 80°C for 10 min (38) demonstrated the highest fluorescence intensity and least background noise (data not shown); thus, these conditions were chosen as the standard for the rest of the study. As in a previous study (32), we found that the SYBR gold-labeled cyanophages produced a number of plaques on host lawn agarose plates equivalent to that obtained with unlabeled phage (see Fig. S2 in the supplemental material).
We next optimized washing conditions to remove unincorporated dye molecules in the viral suspension that might stain host cellular DNA and result in an upward green fluorescence shift in the absence of viruses or viral tagging. Initially, recovery of viral particles after washing was <20% efficient when it was carried out with a commonly used 100-kDa ultrafiltration device, but lower-cutoff devices (10 kDa) increased recovery rates to 29% ± 2% (n = 15; see Fig. S3 in the supplemental material). Two treatments improved recovery further (details are presented in Materials and Methods). Briefly, pretreating the ultracentrifugal devices with bovine serum albumin (BSA) increased viral recovery >2-fold (see Fig. S3), while posttreating the ultrafiltration devices with sonication to remove adhering viral particles increased viral recovery ~3-fold (see Fig. S3). The final, optimized washing protocol recovered 89% ± 5% (n = 15) of viral particles per wash and required stained viruses to be washed six times with Tris-EDTA (TE) buffer to ensure adequate removal of residual SYBR.
Given these staining and washing optimizations, we next mixed the FLVs with host cells (29, 27, 30, 31) and screened them with flow cytometry (Fig. 1A and B). Single-cell observations of a single virus-host pair (cyanophage Syn33 and Synechococcus WH7803) using an Amnis ImageStreamX imaging flow cytometer confirmed that FLVs had labeled only the population of cells with increased green fluorescence. These data from >105 cells showed that green-shifted, virus-tagged cells contained autofluorescent cyanobacterial chlorophyll (red) and DNA-stained virus (green pinpoints), while green-negative cells contained only chlorophyll (red; Fig. 1C). Further, a known host (Synechococcus WH7803) was mixed with fluorescently labeled viruses (Syn33) and nonhost cells (Synechococcus WH8020) at equivalent concentrations (WH7803-Syn33-WH8020 = 1:1:1), or the host cells were flooded with viruses or nonhost cells at a 100-fold-higher density (WH7803-Syn33-WH8020 = 1:100:100). Virus-tagged and nontagged cells were sorted separately using flow cytometry and subjected to viral and bacterial DNA extraction. PCR (40 cycles) targeting rpoC1 genes in bacteria WH7803 and WH8020, and the portal protein gene in virus Syn33 was used to assay specificity of the virus-tagged and nontagged cells. Our results demonstrated that VT is specific and sensitive, as the virus-tagged population was composed exclusively of the known host cells, as determined by PCR, even when the known host was greatly outnumbered by virions (Fig. 1D).
Given an optimized VT method, we next sought to document whether a positive VT signal from diverse virus-host systems correlated with the results two existing gold standard infection assays (plaque and liquid infection assays). Five lytic cyanophages (two myo-, two podo-, and one siphoviruses) and six heterophages (viruses that infect heterotrophic Pseudoalteromonas bacteria; one lytic myovirus, one lytic podovirus, and four siphoviruses) were challenged with 12 cyanobacterial and 12 Pseudoalteromonas strains in VT, plaque infection, and liquid infection assays (Fig. 2). For the 264 possible bacterium-virus pairings (24 bacteria × 11 viruses), VT adsorption results matched infection results from plaque and liquid infection assays with the exception of bacterial pairings involving all four Pseudoalteromonas siphoviruses tested. Thus, when the VT signal was positive, so were the infection results, and vice versa, which suggests that for the bulk of the phage-host pairs under the conditions tested, adsorption equals infection. Infection assays indicated that the four exceptional Pseudoalteromonas siphoviruses four siphoviruses infected their specific known host strains, but their VT signals indicated that adsorption was more promiscuous and included both recognized and unrecognized host strains of Pseudoalteromonas. Monitoring adsorption kinetics with one of these Pseudoalteromonas siphoviruses on multiple host strains showed that it adsorbed to both host and nonhost strains (see Fig. S4 in the supplemental material), consistent with the VT signals observed.
We next explored how five hosts and their specific viruses responded to infection conditions that included various virus-to-bacterium ratios (VBR) and host growth phases. All host cells were acclimatized through three inoculations and showed reproducible growth curves before use in the physiology experiments (Fig. 3A).
For the first set of experiments, a constant concentration (~106 cells ml−1) of late-logarithmic-growth-phase cells was mixed with various fold numbers of their specific viruses (VBRs = 0.05, 0.1, 0.5, 1, 5, and 10) (Fig. 3B). We found that viruses adsorbed to heterotrophic Pseudoalteromonas cells and cyanobacterial cells differently. Pseudoalteromonas viruses adsorbed nearly proportionally to the amount added, until the cells were 100% virus tagged (VBRs > 2) after only 20 min of mixing. In contrast, the cyanophages never tagged more than ~40 to 50% of their host cells even at high VBRs (up to 10) and lengthened adsorption times (up to 60 and 120 min) (see Fig. S5 in the supplemental material).
In the second set of experiments, cells from three physiological growth phases (early log, late log, and stationary) were challenged with their specific viruses using a constant cell concentration of ~106 ml−1 and VBRs of 1 and 10 for Pseudoalteromonas-virus pairs and Synechococcus-virus pairs, respectively (Fig. 3C). Again, we found contrasting results between these two virus-host systems; host cell growth phase was relatively constant in cyanobacterium-virus pairs but not in Pseudoalteromonas-virus pairs, where the percentage of tagged cells was lower in both early log and stationary phases than in the late log phase.
Here we built on previous studies that specifically label bacterial host cells (including Cyanobacteria and E. coli in complex microbial communities using FLVs [30, 31, 39]) by coupling flow cytometry analyses to FLV washing conditions, which enabled specific, high-throughput linkage between viruses and the microbes to which they adsorb.
For most of the viruses tested (all cyanophages and myo- and podovirus heterophages), VT signals were equivalent to infection assay results, suggesting that adsorption of these ocean viruses might always lead to infection. However, for all four heterosiphoviruses this was not the case. We envision two possible mechanistic explanations for this. First, these viruses may adsorb more promiscuously to nonhost cells. There is, however, some specificity to these interactions, as heterosiphoviruses bound only to Pseudoalteromonas strains, not to cyanobacteria (Fig. 2). Some viruses bind irreversibly to a single receptor (e.g., podovirus N4 to NfrA ), while other viruses reversibly bind primary receptors that then trigger structural rearrangements of the virus to irreversibly bind a second receptor (e.g., myovirus T4 ). Perhaps the Pseudoalteromonas siphovirus-positive VT signals capture the reversible adsorption step. Alternatively, adsorption may be specific but not result in lytic infection under our plaque or liquid infection assay conditions. Given that siphoviruses are commonly temperate (i.e., capable of entering the lysogenic or prophage state), though at least one of these lacks a readily identifiable integrase gene (PSA-HS4 ), they may form nonlytic infections with some or all of the hosts to which they adsorb. While prophage states present a complex VT signal, such cryptic temperate phage infections remain one of the most challenging aspects of phage biology to study in spite of their documented importance in the wild (e.g., 40 to 60% of marine bacteria contain inducible prophage ). If follow-up work demonstrates that these siphoviruses indeed form lysogenic infections, we posit that VT coupled to traditional growth assays may aid in highlighting potential lysogenic infections that are undetectable by conventional infection assays. If such studies suggest the opposite, then one must cautiously interpret the relationship of adsorption and infection for these more challenging virus-host groups.
Modeling suggests that fast-growing bacteria may exist in low abundances in the wild due to intensified viral predation (e.g., 44). Indeed, both mesocosm experiments and observations of ocean viral abundances support these results. In mesocosms, reduced viral abundance correlates with rare bacterial lineages becoming dominant (45), while broad ocean surveys show that viral abundances are commonly negatively correlated with (presumably fast-growing) heterotrophic bacteria and positively correlated with slow-growing cyanobacteria (for example, see references 19, 22, 23, and 46 to 48).
Mechanistically, one could imagine fast growers outcompeting co-occurring microbes for nutrients using a high-affinity nutrient transporter that doubles as a viral receptor. Indeed, marine microbial genomes and metagenomes suggest increased transporter diversity among fast-growing relative to slower-growing bacteria (34, 49–51), and new naming conventions have been proposed, such as “opportunitroph” and “passive oligotroph” (51) and “oligotroph” and “copiotroph” (50). Relevant to the host strains examined here, 18 marine cyanobacterial strains (7 Synechococcus and 11 Prochlorococcus) and the only Pseudoalteromonas strain (Pseudoalteromonas atlantica T6c) analyzed in this way are defined as oligotrophs and copiotrophs, respectively (50). In the viral literature (for example, see the review in reference 36), it is posited that such fast-growing microbes are r-selected species (52), with high metabolic rates and rapid response times but low natural abundances due to increased top-down pressures from grazers and viruses. In contrast, slower-growing, K-selected passive oligotrophs are thought to be more resistant to viral and grazing predation, which was empirically measured for cyanobacteria and their viruses decades ago (48). While they are based upon limited data, and clearly not the focus of this study, our observations here that all Pseudoalteromonas cells and only a portion of cyanobacterial cells could be virus tagged are consistent with copiotrophs’ susceptibility and oligotrophs’ resistance to their co-occurring viruses.
Our observation that the number of Pseudoalteromonas-virus pairs, assessed as the percentage of tagged cells, is lower in both early log and stationary phases than late log phase is consistent with previous research with other heterotrophic virus-host systems (53). In these systems (E. coli and Pseudomonas), increases in viral adsorption with host growth rate is thought to be due to a change in the abundance and structure of the cell surface receptor sites. Perhaps similar mechanisms underpin our observations.
In contrast, host growth phase did not impact the cyanobacterium-virus interaction in one of the virus-host systems studied and only minimally impacted it in the other. Two surveys of wild populations in marine systems reported constant viral adsorption rates irrespective of the physiological status of cells (30, 33), which was later interpreted as a “bet-hedging strategy” (54). Such bet-hedging implies that some viruses may attach to host cells regardless of the host’s physiological state, to maximize the probability of the viral population’s producing progeny under starved or challenging conditions when few host cells are proximal. If such a bet-hedging strategy exists and implicates genetic variation in the pool of viral progeny, then perhaps that may explain why not all cyanobacterial cells could be virus tagged. In the oceans, where cyanobacterial cells are abundant, the fraction that are hosts for any particular virus is likely to be very low, with contact rate estimates, guided by likely imperfect culture-dependent strain-specific cyanophage titers, suggesting that every virus-host contact leads to infection (5, 47, 48, 55). Perhaps the overall cyanophage population engages in bet hedging, given the relatively low abundances of cyanobacteria that serve as host cells in open ocean environments.
Alternatively, two methodological issues may have resulted in the observed patterns among cyanobacterial virus-host systems. First, we may have failed to accurately recognize the later stages of cyanobacterial host cell growth. We used fluorescence as a proxy for cyanobacterial biomass, which is susceptible to changes in chlorophyll per cell over different growth phases in batch culture (56). While subsequent comparison of fluorescence-based and cell count-based growth curves suggests that we did not assay stationary-phase cells in our VT experiments (see Fig. S6 in the supplemental material), it remains a robust and intriguing observation that one virus-host pairing showed that early-log-phase cells are virus tagged differently than late-log-phase cells, while the other did not (Fig. 3C). Second, our studies were conducted with host cells grown under continuous light, as opposed to natural, diurnal varying light levels or even on-off light-dark cycles. Light quality impacts adsorption of myovirus S-PM2 to Synechococcus WH7803 (57), and photosynthesis is commonly required for productive cyanophage infections (58, 59). While we achieved consistent growth rates for the cyanobacteria in this study (Fig. 3A), the conditions were not optimized for maximal cell growth or photosynthesis. Future work focusing on evaluating the VT signal in cyanobacterium-virus model systems with axenic cells grown under a light-dark cycle should help differentiate biology from artifact.
Contrasting trophic strategies (r- and K-selected copiotrophs and oligotrophs) among marine bacteria are now suggested by genomic and metagenomic analyses (3, 36, 50, 51, 60). Our data suggest similarly divergent strategies for their viruses that may reflect adaptation to the fundamentally opposing ecological lifestyles of their hosts. Clearly, future work with other model systems will determine whether such inferences about viral life strategies can be generalized. Further, interactions between cyanobacteria and their cocultured heterotrophs were recently highlighted as being critical to cyanobacterial growth (61–63), likely by heterotrophs protecting cyanobacteria from photosynthesis-generated oxygen radicals (61), an evolutionary scenario described as the “Black Queen hypothesis” (64). How viruses play into such coculture experiments would add a new layer to this emerging research trajectory, and VT provides a method that would be invaluable for high-throughput study of these interactions.
The viral ecologist’s toolkit is stronger than ever. For example, the process from source waters to viral metagenome is more robust, as new concentration methods capture nearly all SYBR-stainable particles (65), as well as providing solutions for the limiting DNA problem (66) and empirical data for making decisions about replication and commonly used concentration and purification methods (67). Looking forward, we now envision that VT in combination with other emerging experimental tools in viral ecology—e.g., single viral genomics (68), phageFISH (E. Allers, C. Moraru, M. Duhaime, E. Beneze, N. Solonenko, J. B. Canosa, R. Amann, and M. B. Sullivan, submitted for publication), and microfluidic digital PCR (26)—will prove transformative for the field. These emerging methods, and undoubtedly others, along with parallel innovative informatic solutions should lead to powerful and complementary new windows into viral biology and unprecedented insights into the population structure of viruses in nature, including directly linking viruses to their hosts en masse. We can finally begin to determine “who infects whom” on a scale that allows the development of predictive models of the foundational biological entities (microbes and viruses) in ocean and earth systems. In clinical settings, quantifying viral diversity linked to a particular host is critical for understanding disease progression (69), designing vaccines (70), and developing antiviral drug therapy (71), and VT offers potential contributions to these clinical endeavors.
Viral particles were enumerated as previously described (72). In all samples, at least 500 viral or bacterial particles were counted to get accurate numbers for estimating VBR.
Phage isolates were screened (in triplicate) for their ability to grow on 12 cyanobacterial and 12 Pseudoalteromonas hosts using three different methods. First, liquid infection host range analyses were performed in a volume of 300 μl (VBR = 10; concentrations determined by SYBR gold) in 96-well microtiter plates and analyzed using an Appliskan plate reader as described in the “Culturing conditions” section in Text S1 in the supplemental material. Second, plaque assays at a VBR of 10 were used to confirm the observed host range under liquid infection experimental conditions. Host cell suspensions immobilized in agarose (for Prochlorococcus and Synechococcus hosts) or agar (for Pseudoalteromonas) were incubated under host growth conditions either overnight (Pseudoalteromonas) or 3 weeks (Prochlorococcus and Synechococcus). Third, the VT assay was conducted with each phage-host pair as described below but at a VBR of 10 and bacterial concentrations of 106 ml−1.
Viruses were mixed with bacteria in late logarithmic phase at concentrations of ~108 cells ml−1 and a VBR of 0.1 to optimize adsorption of all viruses. Subsamples were taken at 0, 5, 10, and 30 min after virus addition and immediately filtered (0.2 μm) to remove bacteria, and the filtrate was used in plaque assays to determine the number of free viruses in the medium at each time point. The percentage of free viruses was calculated from the decline in free viruses at each time point relative to the number at time zero.
Viral lysates were purified using a cesium chloride step gradient (2 ml of 1.65 g ml−1, 3 ml of 1.4 g ml−1, 3 ml of 1.3 g ml−1, and 1 ml of 1.2 g ml−1 in 0.02-μm-filtered and autoclaved SNAX medium). Gradients were ultracentrifuged in a Beckman LM-80M with an SW28 rotor at 24,000 rpm for 4 h at 4°C. The resulting purified virus fraction was dialyzed into modified TM storage buffer (600 mM NaCl, 100 mM MgCl2, 100 mM Tris-HCl; pH 7.5). Purified, dialyzed viral samples were used for optimizing staining and washing procedures, and all stains, reagents, and buffers were filter sterilized using 0.02-μm-pore-size Anotop disposable syringe filters (catalog no. 09-926; Whatman Inc.).
See Results for a description of the optimization of the VT stain and washing conditions. To maximize viral recovery from washing steps, two treatments were required: (i) pretreating ultracentrifugal devices (10-kDa cutoff; Nanosep, catalog no. 29300-608; Pall, New York, NY) with 0.5 ml of 0.2-μm-filter-sterilized 1% BSA (catalog no. E531-1.5ML; Bioexpress, Kaysville, UT) in phosphate-buffered saline (PBS) incubated for 1 h at room temperature; and (ii) posttreating the ultrafiltration devices by adding back 50 μl TE buffer and sonicating (VWR Signature ultrasonic cleaner; B1500A-DTH) for 3 min using settings of 50 W at 42 kHz.
Stained and washed viruses were mixed with bacteria at the concentrations and ratios desired for flow cytometer analysis as the second component of the VT assay. All VT experiments were done with a negative control, which was prepared identically to the stained and washed virus samples except without viruses; this controlled for the appearance of cells that were false-positive for virus tagging as a result of free dye.
Bacterial and viral samples were examined using an iCyt Reflection flow cytometer equipped with a 200-mW 488-nm air-cooled solid-state laser. Fluorescence was detected using a 520/40 band pass filter with an amplified photomultiplier tube. Events were detected using a forward scatter trigger, and data were collected in logarithmic mode and then analyzed with WinList 6.0 software (Verity Software House). Fluorescent polystyrene Flow Check microspheres (1 µm yellow-green beads; catalog no. 23517-10; Polysciences Inc., Warrington, PA) were used as an internal standard.
In separate experiments, single cells were imaged and documented using an ImageStreamX imaging flow cytometer with two lasers (488 nm and 10 mW; 785 nm and 5 mW; Amnis Corporation). Images were collected and analyzed using IDEAS 4.0 software (Amnis Corporation).
For a description of the PCR screening, see the supplemental material.
Supplemental materials and methods. Download Text S1, DOCX file, 0.1 MB.
Schematic overview of VT staining optimizations: three dyes (SYBR gold, SYBR green II, and SYBR Safe) were compared under various incubation conditions (80°C for 10 min, room temperature for 10 min, and 4°C for overnight) to identify the optimal VT conditions. All experiments were conducted with the cyanomyovirus Syn33 and the host strain Synechococcus WH7803. Download Figure S1, DOCX file, 0.1 MB.
PFU of SYBR gold-labeled (80°C, 10 min) viruses and unlabeled ones on their host lawns. Each test was done in triplicate (error bars show standard deviations). Download Figure S2, DOCX file, 0.1 MB.
Recovery rate of viruses (cyanomyoviruses Syn33 and S-SM1; Pseudoalteromonas viruses myovirus PSA-HM3, podovirus PSA-HP1, and siphovirus PSA-HS4) using ultracentrifugal devices (Nanosep with low-protein-binding membranes; Pall, New York, NY) with a 10,000 or 100,000 molecular weight cutoff (MWCO) under various conditions. Treatments include pretreatment with bovine serum albumin (BSA) and posttreatment by sonication (for details, see Materials and Methods). Each test was done in triplicate (error bars show standard deviations). Download Figure S3, DOCX file, 0.1 MB.
Adsorption kinetic experiments of Pseudoalteromonas siphovirus PSA-HS6 against four Pseudoalteromonas strains revealed that this siphovirus adsorbs to all four strains. As inferred from traditional plaque and liquid infection assays, this siphovirus infects two of these cell strains (host; solid lines), 11-68 and 13-15, but not the other two (nonhost; dashed lines), 10-33 and H106. Each test was done in duplicate (error bars show standard deviations) using a ratio of 1 virus per 10 bacteria at cell concentrations of 106 cells ml−1. Download Figure S4, DOCX file, 0.1 MB.
Percentage of Synechococcus WH7803 cells tagged by phage Syn33 using VT at a VBR of 10 at 0, 30, 60, and 120 min after mixture of bacteria and viruses. Each test was done in triplicate (error bars show standard deviations) at concentrations of 106 cells ml−1. Download Figure S5, DOCX file, 0.1 MB.
This work was supported by the Biosphere 2, BIO5, NSF OCE0940390, and a Gordon and Betty Moore Foundation grant to M.B.S. The work conducted by the U.S. Department of Energy Joint Genome Institute was supported by the Office of Science of the U.S. Department of Energy under contract no. DE-AC02-05CH11231.
We thank J. B. Waterbury and A. Wichels for Synechococcus and Pseudoalteromonas strains, respectively; Tucson Marine Phage Lab members for discussions and comments on the manuscript; and B. Nankivell for help with figures. We acknowledge Brian Hall and Amnis Corporation for data generation and iCyt and AZCC/ARL-Division of Biotechnology Cytometry Core Facility for cytometry support (Cancer Center Support Grant CCSG–CA 023074).
Citation Deng L, Gregory A, Yilmaz S, Poulos BT, Hugenholtz P, and Sullivan MB. 2012. Contrasting life strategies of viruses that infect photo- and heterotrophic bacteria, as revealed by viral tagging. mBio 3(6):e00373-12. doi:10.1128/mBio.00373-12.