The Vitek-2 yeast susceptibility system (bioMérieux, Marcy l'Étoile, France) is a fully automated commercial method that allows determination of the minimal inhibitory concentration (MIC) of 4 antifungal agents, i.e., amphotericin B, fluconazole, voriconazole, and flucytosine [
1,
2]. This system has demonstrated a high level of reproducibility and has an excellent categorical agreement with the CLSI microdilution reference method [
1-
3]. Moreover, a recent study showed that the Vitek-2 system is superior to the CLSI or the European Committee on Antimicrobial Susceptibility Testing (EUCAST) broth microdilution method for detecting amphotericin B-resistant
Candida isolates [
4]. Because of its advantages, including a significant reduction in technologist hands-on time and turnaround time [
1,
2], this system has become one of the most widely used antifungal susceptibility testing systems in Korean clinical laboratories. However, nationwide data for the
in vitro antifungal susceptibility of
Candida bloodstream infection (BSI) isolates as determined by the Vitek-2 system are still lacking in Korea. In this study, we investigated the
in vitro activity of 4 antifungal agents using the Vitek-2 system against
Candida BSI isolates recovered from 15 tertiary hospitals in Korea.
During the study period (September 2007 to August 2008), 636
Candida BSI isolates were collected from 636 patients among the 15 tertiary hospitals in Korea. Antifungal susceptibility testing with the Vitek-2 system was performed according to the manufacturer's instructions [
1,
2]. The categorical result was obtained according to the breakpoints provided by the Vitek-2 system for amphotericin B (susceptible [S], ≤1 µg/mL; intermediate, 2 µg/mL; resistant [R], ≥4 µg/mL), fluconazole (S, ≤8 µg/mL; susceptible dose dependence [SDD], 16 to 32 µg/mL; R, ≥64 µg/mL), and voriconazole (S, ≤1 µg/mL; SDD, 2 µg/mL; R, ≥4 µg/mL), and flucytosine (S, ≤4 µg/mL; intermediate, 8-16 µg/mL; R, ≥32 µg/mL).
summarizes the in vitro antifungal susceptibilities of the 636 Candida BSI isolates to 4 antifungal agents as determined by using the Vitek-2 system. For all 636 Candida BSI isolates combined, the activity of each agent (µg/mL), expressed as the MIC50/MIC90 (and the percentage of susceptible isolates) was as follows: amphotericin, 0.5/0.5 (98.1%); fluconazole, ≤1/2 (95.9%); voriconazole, ≤0.12/≤0.12 (99.1%); and flucytosine, ≤1/≤1 (97.3%). These results represent the updated nationwide data, which show that the majority of Candida BSI isolates was susceptible to all 4 fungal agents tested.
| Table 1In vitro antifungal susceptibilities of 636 isolates of Candida species to fluconazole, voriconazole, amphotericin B, and flucytosine, as determined by using the Vitek-2 system |
In our previous multicenter study, nearly all isolates (99.7%) had a MIC ≤1 µg/mL for amphotericin B [
5]. In the present study, resistance to amphotericin B was found in 5
Candida isolates; whereas intermediate resistance was found in 7 isolates. These Vitek-2 results produced no major errors when the E test was used as reference standard, supporting our previous report that Vitek-2 is more sensitive for detecting amphotericin B resistance among
Candida species than the CLSI method [
4].
According to the SENTRY Antimicrobial Surveillance Program [
6], the percentages of
Candida BSI isolates with resistance to fluconazole and voriconazole are 2.5% and 1.2%, respectively. In the present study, resistance to fluconazole was found in 1.4% (9/636) of the
Candida isolates (4
C. krusei, 3
C. glabrata, and 2
C. parapsilosis) and resistance to voriconazole was found in 0.9% (6/636) of the
Candida isolates (1
C. albicans and 5
C. glabrata), which was comparable with our previous report [
5].
Until now, nationwide data on the
in vitro antifungal activity of flucytosine against
Candida BSI isolates were not available in Korea. Results from the global SENTRY Antimicrobial Surveillance Program (2008) showed that 95.5% of the 1,201
Candida BSI isolates from 5 continents are susceptible to flucytosine [
6]. Resistance to flucytosine was noted in 2.4% of
C. albicans, 0% of
C. glabrata, 0.5% of
C. parapsilosis, and 10.3% of
C. tropicalis isolates [
6]. In this study, we showed, for the first time, that 97.3% of
Candida BSI isolates from Korea were susceptible to flucytosine, as determined by using the Vitek-2 system. Only 3.2% of
C. albicans, 1.5% of
C. parapsilosis, 1.4% of
C. glabrata, and 0% of
C. tropicalis isolates were resistant to this agent. Again, our results show that the low rates of resistance to flucytosine are consistent with reports from other countries.
2 Hyun-Jung Choi, M.D.,2 Min-Young Ju, M.S.,2 Soo-Hyun Kim, M.D.,2 Wee Gyo Lee, M.D.,3 Yeon-Joon Park, M.D.,4 Kyungwon Lee, M.D.,5 and Korean Study Group for Candidemia