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BMC Med. 2012; 10: 98.
Published online Aug 31, 2012. doi:  10.1186/1741-7015-10-98
PMCID: PMC3482562
Toll-like receptor 4 signaling promotes epithelial-mesenchymal transition in human hepatocellular carcinoma induced by lipopolysaccharide
Ying-Ying Jing,#1 Zhi-Peng Han,#1 Kai Sun,#1 Shan-Shan Zhang,1 Jing Hou,1 Yan Liu,1 Rong Li,1 Lu Gao,1 Xue Zhao,1 Qiu-Dong Zhao,1 Meng-Chao Wu,1 and Li-Xin Weicorresponding author1
1Tumor Immunology and Gene Therapy Center, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, PR China
corresponding authorCorresponding author.
#Contributed equally.
Ying-Ying Jing: jingy4172/at/sohu.com; Zhi-Peng Han: hanzhipeng0311/at/126.com; Kai Sun: zhesuk621/at/126.com; Shan-Shan Zhang: drzhangss/at/163.com; Jing Hou: rena_hou/at/126.com; Yan Liu: liuyansunny/at/163.com; Rong Li: Yolanda2158/at/yahoo.com.cn; Lu Gao: mouse.520/at/163.com; Xue Zhao: xue851014/at/126.com; Qiu-Dong Zhao: zhaoqd/at/gmail.com; Meng-Chao Wu: wumengchao2012/at/126.com; Li-Xin Wei: weilixin/at/yahoo.com
Received February 15, 2012; Accepted August 31, 2012.
Abstract
Background
The endotoxin level in the portal and peripheral veins of hepatocellular carcinoma (HCC) patients is higher and lipopolysaccharide (LPS), a cell wall constituent of gram-negative bacteria, has been reported to inhibit tumor growth. However, in this study, we found that LPS-induced toll-like receptor 4 (TLR4) signaling was involved in tumor invasion and survival, and the molecular mechanism was investigated,
Methods
Four HCC cell lines and a splenic vein metastasis of the nude mouse model were used to study the invasion ability of LPS-induced HCC cells and the epithelia-mesenchymal transition (EMT) in vitro and in vivo. A total of 106 clinical samples from HCC patients were used to evaluate TLR4 expression and analyze its association with clinicopathological characteristics
Results
The in vitro and in vivo experiments demonstrated that LPS could significantly enhance the invasive potential and induce EMT in HCC cells with TLR4 dependent. Further studies showed that LPS could directly activate nuclear factor kappa B (NF-κB) signaling through TLR4 in HCC cells. Interestingly, blocking NF-κB signaling significantly inhibited transcription factor Snail expression and thereby inhibited EMT occurrence. High expression of TLR4 in HCC tissues was strongly associated with both poor cancer-free survival and overall survival in patients.
Conclusions
Our results indicate that TLR4 signaling is required for LPS-induced EMT, tumor cell invasion and metastasis, which provide molecular insights for LPS-related pathogenesis and a basis for developing new strategies against metastasis in HCC.
Keywords: Toll-like receptor 4, Epithelial-mesenchymal transition, Lipopolysaccharide, Human hepatocellular carcinoma
Articles from BMC Medicine are provided here courtesy of
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