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Infect Immun. 1983 March; 39(3): 1392–1402.
PMCID: PMC348110

Alterations in the pathogenicity of Escherichia coli K-12 after transfer of plasmid and chromosomal genes from Shigella flexneri.

Abstract

A 140-megadalton plasmid (pWR110), which has previously been associated with virulence in Shigella flexneri, was transferred to Escherichia coli K-12. Segments of S. flexneri chromosomal material were then transferred to the plamid-bearing K-12 strains. The virulence of these transconjugant hybrids was assessed in the HeLa cell model, in ligated rabbit ileal loops, or in the Sereny test. A K-12 strain which harbored only pWR110 invaded HeLa cells, produced minimal lesions in the rabbit ileal mucosa, and was negative in the Sereny test. Plasmid-containing K-12 hybrids which had incorporated various shigella chromosomal regions gave differential reactions in the rabbit ileal loops and in the Sereny test. Analysis of these transconjugants indicated that three regions were linked with virulent phenotypes. These included the his region (when the genes responsible for O-antigen synthesis were cotransferred) and the kcp locus (linked to the lac-gal region). Either of these chromosomal regions was sufficient to allow invasion of the rabbit ileal mucosa. In addition to both of these regions, another shigella chromosomal segment linked to the arg and mtl loci was necessary for fluid production in the rabbit ileal loop and for a positive Sereny reaction. Thus, derivatives of an E. coli K-12 strain, constructed by the stepwise conjugal transfer of a large plasmid and three chromosomal segments from S. flexneri, appeared to contain the necessary determinants for full pathogenicity in a variety of laboratory models.

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Selected References

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  • FALKOW S, SCHNEIDER H, BARON LS, FORMAL SB. VIRULENCE OF ESCHERICHIA-SHIGELLA GENETIC HYBRIDS FOR THE GUINEA PIG. J Bacteriol. 1963 Dec;86:1251–1258. [PMC free article] [PubMed]
  • Alcalá J, Maisel H. Specific antiserum to the main intrinsic polypeptide of chick lens fiber cell plasma membranes. Exp Eye Res. 1978 Feb;26(2):219–221. [PMC free article] [PubMed]
  • Formal SB, Hornick RB. Invasive Escherichia coli. J Infect Dis. 1978 May;137(5):641–644. [PubMed]
  • Formal SB, Kent TH, May HC, Palmer A, Falkow S, LaBrec EH. Protection of monkeys against experimental shigellosis with a living attenuated oral polyvalent dysentery vaccine. J Bacteriol. 1966 Jul;92(1):17–22. [PMC free article] [PubMed]
  • FORMAL SB, KUNDEL D, SCHNEIDER H, KUNEVN, SPRINZ H. Studies with Vibrio cholerae in the ligated loop of the rabbit intestine. Br J Exp Pathol. 1961 Oct;42:504–510. [PubMed]
  • FORMAL SB, LABREC EH, KENT TH, FALKOW S. ABORTIVE INTESTINAL INFECTION WITH AN ESCHERICHIA COLI-SHIGELLA FLEXNERI HYBRID STRAIN. J Bacteriol. 1965 May;89:1374–1382. [PMC free article] [PubMed]
  • FORMAL SB, LABREC EH, FALKOW S. RESTORATION OF VIRULENCE TO A STRAIN OF SHIGELLA FLEXNERI BY MATING WITH ESCHERICHIA COLI. J Bacteriol. 1965 Mar;89:835–838. [PMC free article] [PubMed]
  • Goldman RC, Leive L. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2. Eur J Biochem. 1980;107(1):145–153. [PubMed]
  • Hale TL, Bonventre PF. Shigella infection of Henle intestinal epithelial cells: role of the bacterium. Infect Immun. 1979 Jun;24(3):879–886. [PMC free article] [PubMed]
  • Hale TL, Formal SB. Protein synthesis in HeLa or Henle 407 cells infected with Shigella dysenteriae 1, Shigella flexneri 2a, or Salmonella typhimurium W118. Infect Immun. 1981 Apr;32(1):137–144. [PMC free article] [PubMed]
  • Hale TL, Morris RE, Bonventre PF. Shigella infection of henle intestinal epithelial cells: role of the host cell. Infect Immun. 1979 Jun;24(3):887–894. [PMC free article] [PubMed]
  • Kim R, Corwin LM. Factors affecting virulence of Shigella flexneri: avirulent strain with altered metablism of succinate, fumarate, and malate. Infect Immun. 1973 Apr;7(4):625–630. [PMC free article] [PubMed]
  • Kopecko DJ, Washington O, Formal SB. Genetic and physical evidence for plasmid control of Shigella sonnei form I cell surface antigen. Infect Immun. 1980 Jul;29(1):207–214. [PMC free article] [PubMed]
  • Jain SK, Subrahmanyam D. Two dimensional thin-layer chromatography of polar lipids. Ital J Biochem. 1978 Jan-Feb;27(1):11–18. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Okamura N, Nakaya R. Rough mutant of Shigella flexneri 2a that penetrates tissue culture cells but does not evoke keratoconjunctivitis in guinea pigs. Infect Immun. 1977 Jul;17(1):4–8. [PMC free article] [PubMed]
  • Palva ET, Mäkelä PH. Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Eur J Biochem. 1980;107(1):137–143. [PubMed]
  • Sansonetti PJ, d'Hauteville H, Formal SB, Toucas M. Plasmid-mediated invasiveness of "Shigella-like" Escherichia coli. Ann Microbiol (Paris) 1982 May-Jun;133(3):351–355. [PubMed]
  • Sansonetti PJ, Kopecko DJ, Formal SB. Involvement of a plasmid in the invasive ability of Shigella flexneri. Infect Immun. 1982 Mar;35(3):852–860. [PMC free article] [PubMed]
  • Simmons DA. Immunochemistry of Shigella flexneri O-antigens: a study of structural and genetic aspects of the biosynthesis of cell-surface antigens. Bacteriol Rev. 1971 Jun;35(2):117–148. [PMC free article] [PubMed]
  • Tsai CM, Frasch CE. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. [PubMed]
  • VOINO-IASENETSKII MV, KHAVKIN TN. IZUCHENIE VNUTRI'EPITELIAL'NO I LOKALIZATSII VOZBUDITELE I DIZENTERII PRI POMOSHCHI FLUORESTSIRUIUSHCHIKH ANTITEL. Zh Mikrobiol Epidemiol Immunobiol. 1964 Apr;41:98–100. [PubMed]

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