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Logo of bmcpsBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Plant Biology
 
BMC Plant Biol. 2012; 12: 121.
Published online Jul 29, 2012. doi:  10.1186/1471-2229-12-121
PMCID: PMC3480847
Global transcriptome analysis reveals distinct expression among duplicated genes during sorghum-interaction
Hiroshi Mizuno,#1 Hiroyuki Kawahigashi,#1 Yoshihiro Kawahara,#1 Hiroyuki Kanamori,1 Jun Ogata,1 Hiroshi Minami,2 Takeshi Itoh,1 and Takashi Matsumotocorresponding author1
1National Institute of Agrobiological Sciences (NIAS), Agrogenomics Research Center, 1-2, Kannondai 2-chome, Tsukuba, Ibaraki 305-8602, Japan
2Mitsubishi Space Software Co. Ltd, Takezono 1-6-1, Tsukuba, Ibaraki 305-0032, Japan
corresponding authorCorresponding author.
#Contributed equally.
Hiroshi Mizuno: hmizuno/at/nias.affrc.go.jp; Hiroyuki Kawahigashi: shiwak/at/nias.affrc.go.jp; Yoshihiro Kawahara: y.kawahara/at/affrc.go.jp; Hiroyuki Kanamori: kanamo/at/affrc.go.jp; Jun Ogata: ogata722/at/affrc.go.jp; Hiroshi Minami: Minami.Hiroshi/at/mss.co.jp; Takeshi Itoh: taitoh/at/affrc.go.jp; Takashi Matsumoto: mat/at/nias.affrc.go.jp
Received October 9, 2011; Accepted July 29, 2012.
Abstract
Background
Sorghum (Sorghum bicolor L. Moench) is a rich source of natural phytochemicals. We performed massive parallel sequencing of mRNA to identify differentially expressed genes after sorghum BTx623 had been infected with Bipolaris sorghicola, a necrotrophic fungus causing a sorghum disease called target leaf spot.
Result
Seventy-six-base-pair reads from mRNAs of mock- or pathogen-infected leaves were sequenced. Unannotated transcripts were predicted on the basis of the piling-up of mapped short reads. Differentially expressed genes were identified statistically; particular genes in tandemly duplicated putative paralogs were highly upregulated. Pathogen infection activated the glyoxylate shunt in the TCA cycle; this changes the role of the TCA cycle from energy production to synthesis of cell components. The secondary metabolic pathways of phytoalexin synthesis and of sulfur-dependent detoxification were activated by upregulation of the genes encoding amino acid metabolizing enzymes located at the branch point between primary and secondary metabolism. Coordinated gene expression could guide the metabolic pathway for accumulation of the sorghum-specific phytochemicals 3-deoxyanthocyanidin and dhurrin. Key enzymes for synthesizing these sorghum-specific phytochemicals were not found in the corresponding region of the rice genome.
Conclusion
Pathogen infection dramatically changed the expression of particular paralogs that putatively encode enzymes involved in the sorghum-specific metabolic network.
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