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Allergy Asthma Immunol Res. Nov 2012; 4(6): 367–369.
Published online Jun 15, 2012. doi:  10.4168/aair.2012.4.6.367
PMCID: PMC3479232
Immunologic Evaluation of Ofloxacin Hypersensitivity
Young-Hee Nam,1 Jeong Eun Kim,2 Seung-Hyun Kim,1 Hyun Jung Jin,3 Eui-Kyung Hwang,1 Yoo Seob Shin,1 Young-Min Ye,1 and Hae-Sim Parkcorresponding author1
1Department of Allergy & Clinical Immunology, Ajou University School of Medicine, Suwon, Korea.
2Division of Pulmonology and Critical Care Medicine, Department of Internal Medicine, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon, Korea.
3Department of Internal Medicine, Yeungnam University College of Medicine, Daegu, Korea.
corresponding authorCorresponding author.
Correspondence to: Hae-Sim Park, MD, PhD. Department of Allergy and Clinical Immunology, Ajou University School of Medicine, San 5 Woncheon-dong, Yeongtong-gu, Suwon 443-721, Korea. Tel: +82-31-219-5196; Fax: +82-31-219-5154; hspark/at/ajou.ac.kr
Received March 19, 2012; Revised April 23, 2012; Accepted May 11, 2012.
Abstract
Quinolone hypersensitivity, most of which is immediate type, is rare but has increased in recent years. The pathogenic mechanisms underlying immediate reactions are not defined clearly. This study was aimed to observe the clinical characteristics of immediate hypersensitivity to ofloxacin and to investigate the pathogenic mechanism with detection of serum specific IgE to ofloxacin using an enzyme-linked immunoasorbent assay (ELISA). We recruited 5 patients with immediate hypersensitivity reactions to ofloxacin (group I), and as control groups, 5 subjects with ciprofloxacin hypersensitivity (group II) and 20 healthy subjects with no history of drug allergy. Serum specific-IgE to ofloxacin-human serum albumin (HSA) conjugate was detectable in four group I subjects (80%) and three group II subjects (60%). The ELISA inhibition test showed significant inhibition with both ofloxacin-HSA conjugate and free ofloxacin in a dose-dependent manner. As to ciprofloxacin, significant inhibition was noted upon addition of free ciprofloxacin in one subject, while minimal inhibition was noted in the other. We confirmed that an IgE-mediated response is a major pathogenic mechanism of ofloxacin hypersensitivity. Cross reactivity between ofloxacin and ciprofloxacin was noted with individual difference.
Keywords: Cross reactivity, enzyme-linked immunosorbent assay, IgE, immediate hypersensitivity, ofloxacin
Articles from Allergy, Asthma & Immunology Research are provided here courtesy of
Korean Academy of Asthma, Allergy and Clinical Immunology and Korean Academy of Pediatric Allergy and Respiratory Disease