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Cytotechnology. Mar 2006; 50(1-3): 77–92.
Published online Jul 1, 2006. doi:  10.1007/s10616-006-9008-5
PMCID: PMC3476002
Regulating apoptosis in mammalian cell cultures
Nilou Arden and M. J. Betenbaughcorresponding author
Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218 USA
M. J. Betenbaugh, beten/at/jhu.edu.
corresponding authorCorresponding author.
Received March 31, 2006; Accepted March 31, 2006.
Cell culture technology has become a widely accepted method used to derive therapeutic and diagnostic protein products. Mammalian cells adapted to grow in bioreactors now play an integral role in the development of these biologicals. A major limiting factor determining the output efficiency of mammalian cell cultures however, is apoptosis or programmed cell death. Methods to delay apoptosis and increase the longevity of cell cultures can lead to more economical processes. Researchers have shown that both genetic and chemical strategies to block apoptotic signals can increase cell culture productivity. Here, we discuss various strategies which have been implemented to improve cellular viabilities and productivities in batch cultures.
Keywords: Mammalian cell culture, Apoptosis, Bcl-2 protein, Cell cycle arrest, Recombinant protein production
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