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Logo of anclinmicBioMed CentralBiomed Central Web Sitesearchsubmit a manuscriptregisterthis articleAnnals of Clinical Microbiology and Antimicrobials
 
Ann Clin Microbiol Antimicrob. 2012; 11: 20.
Published online Jul 28, 2012. doi:  10.1186/1476-0711-11-20
PMCID: PMC3475072
Biological activities of extracts from Chenopodium ambrosioides Lineu and Kielmeyera neglecta Saddi
Zulane Lima Sousa,1 Fernando Faustino de Oliveira,2 Aline Oliveira da Conceição,3 Luiz Alberto Mattos Silva,3 Maria Helena Rossi,4 Juliana da Silva Santos,4 and João Luciano Andriolicorresponding author1,3
1Programa de Pós-graduação em Biologia e Biotecnologia de Microrganismos, Universidade Estadual de Santa Cruz, Campus Prof. Soane Nazaré de Andrade, Km 16 – Rodovia Ilhéus/Itabuna, 45662-900, Ilhéus, Brazil
2Departamento de Ciências Exatas e Tecnológicas, Universidade Estadual de Santa Cruz, Campus Prof. Soane Nazaré de Andrade, Km 16 – Rodovia Ilhéus/Itabuna, 45662-900, Ilhéus, Brazil
3Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Campus Prof. Soane Nazaré de Andrade, Km 16 – Rodovia Ilhéus/Itabuna, 45662-900, Ilhéus, Brazil
4Instituto Biológico, Centro de Sanidade Animal, Av. Cons. Rodrigues Alves, 1252, 04014-002, São Paulo, Brazil
corresponding authorCorresponding author.
Zulane Lima Sousa: zulanelima/at/hotmail.com; Fernando Faustino de Oliveira: faustino/at/uesc.br; Aline Oliveira da Conceição: conceicao.aline/at/yahoo.ca; Luiz Alberto Mattos Silva: mattos/at/uesc.br; Maria Helena Rossi: rossi/at/biologico.sp.gov.br; Juliana da Silva Santos: juliana_ssantos/at/yahoo.com.br; João Luciano Andrioli: joaoluciano2002/at/yahoo.com.br
Received May 22, 2012; Accepted July 22, 2012.
Abstract
Background
Chenopodium ambrosioides and Kielmeyera neglecta are plants traditionally used in Brazil to treat various infectious diseases. The study of the biological activities of these plants is of great importance for the detection of biologically active compounds.
Methods
Extracts from these plants were extracted with hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc) and ethanol (EtOH) and assessed for their antimicrobial properties, bioactivity against Artemia salina Leach and antifungal action on the cell wall of Neurospora crassa.
Results
Extracts from C. ambrosioides (Hex, DCM and EtOH) and K. neglecta (EtOAc and EtOH) showed high bioactivity against A. salina (LD50 < 1000 μg/mL), which might be associated with cytotoxic activity against cancer cells. C. ambrosioides Hex and DCM showed specific activity against yeasts, highlighting the activity of hexanic extract against Candida krusei (MIC = 100 μg/mL). By comparing the inhibitory concentration of 50% growth (IC 50%) with the growth control, extracts from K. neglecta EtOAc and EtOH have shown activities against multidrug-resistant bacteria (Enterococcus faecalis ATCC 51299 and Staphylococcus aureus ATCC 43300), with IC 50% of 12.5 μg/mL The assay carried out on N. crassa allowed defining that extracts with antifungal activity do not have action through inhibition of cell wall synthesis.
Conclusions
Generally speaking, extracts from C. ambrosioides and K. neglecta showed biological activities that have made the search for bioactive substances in these plants more attractive, illustrating the success of their use in the Brazilian folk medicine.
Keywords: Antimicrobial, Bioactivity, Extract, Chenopodium ambrosioides, Kielmeyera neglecta, Candida
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