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BMC Cancer. 2012; 12: 219.
Published online Jun 6, 2012. doi:  10.1186/1471-2407-12-219
PMCID: PMC3472177
Promoter hypermethylation-induced transcriptional down-regulation of the gene MYCT1 in laryngeal squamous cell carcinoma
Min Yang,#1,2 Wei Li,#1,3 Yi-Ying Liu,1 Shuang Fu,1 Guang-Bin Qiu,4 Kai-Lai Sun,1 and Wei-Neng Fucorresponding author1
1Department of Medical Genetics, China Medical University, Shenyang, 110001, P.R. China
2Shenyang Police-dog Technology School of Ministry of Public Security, Shenyang, 110034, P.R. China
3ENT Department, the First Affiliated Hospital of China Medical University, Shenyang, 110001, P.R. China
4Department of Laboratory Medicine, No. 202 Hospital of PLA, Shenyang, 110003, P.R. China
corresponding authorCorresponding author.
#Contributed equally.
Min Yang: yangmin19770902/at/163.com; Wei Li: liwei/at/mail.cmu.edu.cn; Yi-Ying Liu: yyliu/at/163.com; Shuang Fu: coolerfspretty/at/126.com; Guang-Bin Qiu: qiuguangbin/at/163.com; Kai-Lai Sun: klsun/at/mail.cmu.edu.cn; Wei-Neng Fu: wnfu/at/mail.cmu.edu.cn
Received March 1, 2012; Accepted June 6, 2012.
Abstract
Background
MYCT1, previously named MTLC, is a novel candidate tumor suppressor gene. MYCT1 was cloned from laryngeal squamous cell cancer (LSCC) and has been found to be down-regulated in LSCC; however, the regulatory details have not been fully elucidated.
Methods
Here, we sought to investigate the methylation status of the CpG islands of MYCT1 and mRNA levels by bisulfite-specific PCR (BSP) based on sequencing restriction enzyme digestion, reverse transcription and real-time quantitative polymerase chain reaction (RQ-PCR). The function of specific sites in the proximal promoter of MYCT1 in LSCC was measured by transient transfection, luciferase assays, electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation assay (ChIP).
Results
The results suggested hypermethylation of 12 CpG sites of the promoter in both laryngeal cancer tissues and the laryngeal cancer line Hep-2 cell. The hypermethylation of the site CGCG (−695 to −692), which has been identified as the c-Myc binding site, was identified in laryngeal cancer tissues (59/73) compared to paired mucosa (13/73); in addition, statistical analysis revealed that the methylation status of this site significantly correlated with cancer cell differentiation(p < 0.01). The mRNA level of MYCT1 increased in Hep-2 cells treated with 5-aza-C (p < 0.01). The luciferase activity from mutant transfectants pGL3-MYCT1m (−852/+12, mut-695-C > A, mut-693-C > G) was significantly reduced compared with the wild type pGL3-MYCT1 (−852/+12), while the luciferase activity from wild transfectants pGL3-MYCT1 (−852/+12) rose after 5-aza treatment in Hep-2 cells. Finally, EMSA and ChIP confirmed that the methylation of the CGCG (−695 to −692) site prevented c-Myc from binding of the site and demethylation treatment of the 5′ flanking region of MYCT1 by 5-aza induced the increased occupation of the core promoter by c-Myc (p < 0.01).
Conclusion
In summary, this study concluded that hypermethylation contributed to the transcriptional down-regulation of MYCT1 and could inhibit cancer cell differentiation in LSCC. DNA methylation of the CGCG site (−695 to −692) of MYCT1 altered the promoter activity by interfering with its binding to c-Myc in LSCC. Epigenetic therapy of reactivating MYCT1 by 5-aza should be further evaluated in clinical trails of LSCC.
Keywords: Hypermethylation, MYCT1, Laryngeal squamous cell carcinoma
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