The present investigation is probably the first one among apparently healthy individuals in Brazil, as well as, among native Indians and Afro-descendant groups (originated from escapee slaves during the XIX century) in the North region of the Brazilian Amazon region.
The sex composition was balanced and followed the demographics of the city of Belem, the largest and most important city of the North region of the country 
. Several available studies do not inform the ages of the infected persons, except for some studying population groups from Africa 
, North America 
, Europe 
, Australia 
and North Korea 
. It is believed that primary infection occurs early in childhood 
, consequently, it is necessary to keep monitoring the infection among different age groups of different populations, healthy or not, once JCV is a persistent infection which is largely disseminated among human groups 
Among the Afro-descendants and the residents in Belem, older than 30 years of age, 65% and 66%, respectively, were infected, similarly to what has been described when testing new diagnostics kits, in Australia and among persons with non-Hodgkin lymphoma 
. Although there are few studies among young individuals, the number of samples is low and the available volume of urine does not favour the amplification of DNA 
JCV infection was defined by the detection of two genetic codifying areas (VP1 and IG regions), which present higher heterogeneity as compared to the regulatory region. The use of molecular biology assays is in agreement with seroepidemiological studies to determine the prevalence of the virus 
. Most of the results consider the presence of IG 
, but its size (610 bp) is not advantageous and may compromise the results 
. The use of a smaller region (VP1) is equally useful to describe the occurrence and the estimates of prevalence rates of infection by JCV among human populations 
In the present paper it was possible to amplify both regions in 77%, 52% and 16% of samples from Amerindians, urban groups and Afro-descendants, respectively. The efficiency of amplification of IG is highly dependent on the supercoiling nature of polyomaviruses 
. Another possibility may be the small number of copies of DNA present in the samples, as well as, the small amounts of urine available for the isolation of DNA, which could lead to low levels of detection 
. The lower levels of prevalence found among the samples originated from Afro-descendants might have had some inadequacies during transportation regarding the time elapsed from collection to arrival in the laboratory and the conditions of temperature during transportation. Such conditions might prevent more significant interpretations of the frequency rate found in the group.
The prevalence of JCV among residents in Belem (33%) is comparable to those found in Australia (29%) 
, among urban populations in the USA (40%) 
, and among European countries (range from 30% to 44%) 
. It is common to find low prevalence rates among groups residing in South California, USA (18%) 
, North Korea (20%) 
and Norway (21%) 
, as well as higher ones in the Philipines (47%) 
and among Japanese descendants residing in the USA (53%) 
The presence of JCV in one Surui Indian woman is a good indicator that the virus may be circulating among the community as they still are an epidemiologically semi-closed population. The small number of individuals examined may have accounted for the sole finding. A large variation of prevalence rates has been reported in the Americas that includes the Inuits in Canada (26%) 
, among the Navahos (66%) and the Flathead (56%) in the USA, rates which were similar to those found (69%) in the Chamorro Pacific, Micronesia 
, in Asian populations (65%) 
, in Australia (33% in urban groups and 28% among natives) 
and within native populations from Siberia, with rates of excretion ranging from 13% (Chuckchis), 35% (Luskys and Yukaghirs), 39% (Koryaks) to 56% in the Nanais 
JCV infections among Afro-descendants is usually similar. The prevalence among pigmies was 22% and 20% among Bantus 
. An average of 20% was found among other groups of the continent 
, a figure slightly lower than that described in the present paper (40%) and for Afro-Americans (56%) in the USA. The difference may be attributable to the intense miscigenation of Afro-descendants in Brazil and in the USA.
JCV is usually associated to population groups and their migration patterns in the same fashion as with mitochondrial DNA and the Y chromosome 
. There is a strong correlation observed between the multiple viral subtypes and the different population groups, according to their ethnical ancestry 
In Belem, three ancestral types were described, according to its geographical association to the type A (3%), found in Europe, type B (83%), found in Africa and Asia, and type C (14%) found in the African continent. This is in strict adherence to the historical process of formation of the population of urban communities like Belem, as well as to the genetic information available of the composition of the urban population of Belem using classic genetic polymorphisms 
, and molecular genetic markers 
Type B was the most frequently found and samples from Belem which were subtyped as Af2 (66%) were mostly from “mesticos” (84%) who were born in the city as well (56%). It is relevant to mention that 25% of the individuals presented in their demographic records the observation of phenotype characteristics related to a black ethnicity, but without a self referral as such. This is a relevant matter when considering that most of the Af2 genotype evolved within the African continent approximately 50,000 years ago 
. The results show both the African origin of JCV subtype and the origin of the individuals present in a high genetically mixed population.
All samples from Belem classified as subtype MY were originated also from individuals classified as “mesticos”. Two were born in Belem and two were born in two different villages. The samples showed 99% similarity to the MY prototype described among native Americans 
. The samples originated from the “quilombo” populations, two were of subtype Af2 and one of subtype MY. The Surui Indian sample was equivalent to the MY subtype described within native Americans 
The high similarity observed among the samples from urban areas, the native Indians and the prototype isolated from native Americans is suggestive that type B, subtype MY, entered into the Brazilian Amazonian population during its process of formation by the mixture of migration patterns more than 10,000 years associated to the introduction of the European and the incoming African groups.
JCV is shown herein, for the first time, as a strong marker of population migration patterns when the similarity of the American strains are compared, including those of epidemiologically closed communities originated after the crossing of the Behring Strait and settling in the Americas 
. JCV, type B, subtype MY, establishes persistence in the human host and was brought by the migratory Amerindians and later disseminated to the present trihybrid populations residing in the area. Once it was present among the native Indians in the Amazon region, subtype MY could have entered into the original “quilombos” (slave communities formed after their fled from slavery in cities, villages and farms). “Quilombos” date as back as 1788 in the State of Para, Brazil 
and their number increased markedly from the end of the XVIII to the beginning of the XIX centuries. Some genetic studies strongly support this view of ethnical mixture 
In a similar way, the strain was introduced in the city of Belem probably from its origin in 1616 due to the extensive mixture of the three ethnical groups. The contribution of the Indian population within urban areas is approximately 35% and to a lower extent, that of the Afro-descendants 
. Again, the most probable origin of the MY subtype is the link to the North American Indians.
The phylogenetic relationship among JCV strains from Japan, Korea and Amerindians suggests that emergence of subtype MY occurred between 50 and 30 thousand years ago and that within the MY clade between 30 and 10 thousand years 
. The separation of genotypes associated to native American Indians has probably occurred some 15,000 years ago, an estimate which is in agreement with the archeological evidence for the entry of the first humans into the American continent. The proposed model is similar to that which occurred with another human persistently infecting virus, HTLV-2 
The role of JCV as a marker of past and recent human migration and mixture of different ethnic groups is also defined by the detection of type C, in which all samples were identified as subtype Af1. The isolates were originated from individuals with phenotypic and socio demographic informations of being all “mesticos” of whom 60% resembled whites and 40% negros. Most of them were born outside large cities.
Type C represents the ancestral JCV that evolved circa 100 thousand years ago, generating subtype Af1, which was subsequently disseminated into West Africa, an area associated to the emergence of the human species, and later to Central Africa including Central African Republic, Ghana and Mauritania 
. The identification of type C, subtype Af1 among urban residents, again indicates the contribution of the African slaves to the genetic composition of the population. In a similar fashion as type B, subtype Af2, both are homogeneously distributed among the tri-hybrid groups of humans in the Amazon region of Brazil.
It has been proposed that the ancestral JCV emerged together with man (circa 200–100 thousand years ago) in Africa 
. Type C, subtype Af1 is the probable prototype which first emerged, and later, circa 50,000 years, types A and B emerged. When the first humans started their migration patterns, JCV was also disseminated. One possible route, probably the oldest one, suggests that humans hosted JCV ancestral type A in their pathway towards outside Africa. A second migration route, probably took place within the African continent, and included humans hosting ancestral type B of JCV.
The first modification that took place in type B, originated Af2 and the non-Af2 subtypes. Subtype Af2 was then disseminated within the African continent and, secondarily, to Saudi Arabia, the West and South of Asia, as well as to Southeast Europe (circa 50 to 10 thousand years ago). The non-Af2 strains originated the B1-c, a minor European genotype, and the main genotypes present in Asia, Oceania and the Amerindians from North America (B1-a, B1-b, B1-d, B2, CY, MY, SC, 8A, 8B e 2E).
Type A, subtype EU was also detected within the urban population of Belem, from one individual whose relatives dated back to the routes of heavy migration from Portugal during colonization. This recent migration pattern of Europeans, particularly in the last 300 years, enables the role of JCV as a good marker to trace patterns of ancient and recent migration of humans and its genetic mixture with other ethnic groups.
Mutations in positions 2242 nt (C→A/G), 2285 nt (C→G), 2309 nt (A→G), 2362 nt (A→G) and 2407 nt (C→T), may be considered as a signature of the viral subtype after entering the Amazon region of Brazil. Maintenance of JCV is a consequence of persistence in the human host, its moderate genetical stability and the low levels of DNA mutations. The presence of the same pattern of mutations even within a woman from the Surui native Indians, a semi-closed epidemiological community, reinforces the genetic peculiarity of subtype MY in the Amazon region of Brazil and the transmission of the virus from the ancestral human native populations to the Afro-descendants and the present tri-hybrid human populations.
It was unexpected the finding of a nucleotidic sequence of the polyoma virus BK in an Afro-descendant individual, but it was previously described among patients who received bone marrow transplants 
, renal transplant recipients 
and among Chinese healthy adults 
. The amplification of VP1, is useful for the detection of JCV and BKV as well, as they share approximately 72% of nucleotide homology in the studied 
. The presence of both viruses indicate the dissemination of polyomaviruses and the need to improve their clinical detection among human populations.