Overall, less methylation was observed in anti-dsDNA positive patients compared with dsDNA-negative patients. Analysis of the 27 k methylation data demonstrated decreased methylation of a CpG site near SOCS2 (P = 3.5 × 10-7), an inhibitor of the STAT family of transcription factors, as well as a site near GGT1 (P = 4.3 × 10-3). In addition, increased methylation of two CpG sites in PRIC285, a transcriptional co-activator for nuclear receptors, was significantly associated with anti-dsDNA autoantibody production (P = 1.9 × 10-7 and 3.4 × 10-7). Subsequent analysis of 114 methylation sites from the 450 k methylation data in these regions demonstrated very strong association with the methylation site cg22764925, located in the 5'-UTR region of GGT1 (P = 2.8 × 10-11), with an average difference of 7.7% between methylation levels of anti-dsDNA-positive and anti-dsDNA-negative patients. The methylation site cg11738543, and several other CpG sites in the SOCS2 gene, were also associated with anti-dsDNA antibody status (P = 4.2 × 10-8).