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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC Dec 20, 2012.
Published in final edited form as:
PMCID: PMC3467018
NIHMSID: NIHMS388036
A role for presenilins in autophagy revisited: normal acidification of lysosomes in cells lacking PSEN1 and PSEN2
Xulun Zhang,1 Krassimira Garbett,2 Karthikeyan Veeraraghavalu,1 Brian Wilburn,1 Reid Gilmore,3 Karoly Mirnics,2 and Sangram S. Sisodia1*
1Department of Neurobiology, The University of Chicago, Chicago, IL 60637, USA
2Department of Psychiatry, Vanderbilt University, Nashville, TN 37232, USA
3Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
*correspondence: ssisodia/at/bsd.uchicago.edu
Presenilins (PS1 and PS2) are the catalytic subunits of the γ-secretase complex, and genes encoding mutant PS1 and PS2 variants cause familial forms of Alzheimer’s disease (FAD). Lee et al. (2010) recently reported that loss of PS1 activity lead to impairments in autophagosomal function as a consequence of lysosomal alkalinization, caused by failed maturation of the proton translocating V0a1 subunit of the vacuolar (H+)-ATPase and targeting to the lysosome. We have reexamined these issues in mammalian cells and in brains of mice lacking PS (PScdko) and have been unable to find evidence that the turnover of autophagic substrates, vesicle pH, V0a1 maturation or lysosome function is altered compared to wild-type counterparts. Collectively, our studies fail to document a role for presenilins in regulating cellular autophagosomal function. On the other hand, our transcriptome studies of PScdko mouse brains reveal, for the first time, a role for PS in regulating lysosomal biogenesis.
Presenilins are polytopic membrane proteins that serve as the catalytic center of γ-secretase (Ahn et al., 2010), an enzyme complex that catalyzes intramembranous proteolysis of a variety of type I membrane substrates (for review, see De Strooper, 2003). Importantly, inheritance of mutations in the PSEN1 gene and the homologue, PSEN2, causes early onset, autosomal dominant, familial forms of Alzheimer’s disease (FAD).
In addition to a role as the catalytic subunit of “γ-secretase”, studies have shown that PS plays a role in several diverse cellular processes, including autophagy (reviewed in De Strooper B. and Annaert, 2010). In this regard, Lee et al. (2010) reported that in PSEN1-null (PS1ko) blastocysts, and brains of mice with reduced levels of PS1, the degradation of long-lived proteins was impaired, while the clearance of autophagosomes during macroautophagy was prevented. The authors reported that these defects were the consequence of impaired lysosomal acidification resulting from failed oligosaccharide maturation and subsequent transport of the proton translocating V0a1 subunit of the vacuolar (H+)-ATPase (v-ATPase) to the lysosome. At a mechanistic level, the authors reported that full-length PS1 bound to V0a1 subunit in the ER, thus facilitating N-glycosylation by the STT3B subunit of the oligosccharyltransferase (OST) complex. Finally, the authors reported that expression of FAD-linked PS1 variants in patient fibroblasts exhibited impairments in macroautophagy and targeting of V0a1 to lysosomes.
In view of the substantial implications of these latter findings for understanding the functions of presenilins in health and AD, we sought to reexamine these issues. In contrast to the findings reported by Lee et al. (2010), we now document that the turnover of established autophagic substrates is apparently unperturbed in embryonic stem (ES) cells lacking PSEN1 (PS1ko-ES) or both PSEN1 and PSEN2 (PSdko-ES) compared to WT-ES cells. In addition, we demonstrate that mean vesicle pH in cultured PS1ko-ES cells and PSdko-ES cells is very similar to that in cultured WT-ES cells. Indeed, V0a1 maturation and acidic pH-dependent, lysosomal maturation of CatD is indistinguishable between cultured WT-ES, PS1ko-ES and PSdko-ES cells, and in the hippocampi of mice with genetic deletions of PSEN1 and PSEN2 in excitatory neurons (PScdko mice) compared to their littermate controls. Similarly, V0a1 maturation and CatD processing are unimpaired in stable cell lines or brains of transgenic mice expressing PS1 variants. Finally, we neither confirm an association of PS1 holoprotein with either V0a1 and/or STT3B, nor that V0a1 is uniquely glycosylated by STT3B. Collectively, our studies fail to document a role for presenilins in regulating cellular autophagosomal function.
Lastly, we document that the expression of transcripts encoding TFEB, a master transcriptional regulator of lysosomal biogenesis and autophagy, is no different between cultured PSdko-ES and WT-ES cells, or in brains of PScdko mice compared to littermate controls. However, the expression of a set of genes within the Coordinated Lysosomal Expression and Regulation (CLEAR) network is significantly upregulated in the brains of mice lacking PSEN expression in excitatory neurons, findings that would argue that PS plays a critical role in regulating lysosomal biogenesis, but in a TFEB-independent manner.
Cell lines, Plasmids and Transfection
HEK293 cells, wild type, PS1ko and PSdko mouse embryo fibroblasts (MEFs) were cultured in DMEM (Invitrogen, Carlsbad, CA) containing 10% FBS (Invitrogen) and 1X penicillin/streptomycin (Invitrogen). PEN-2 knockdown cells were obtained from Dr. Harald Steiner (Prokop et al., 2004). Early passage WT-ES, PS1ko-ES and PSdko-ES cells were obtained from Dr. Bruce Yankner (Zhang et al., 2000) and were cultured in DMEM containing 15% FBS, 1X non-essential amino acids (Invitrogen), 2mM glutamine (Invitrogen), 1X penicillin/streptomycin, 1mM sodium pyruvate (Invitrogen), 1000units/ml of LIF (Millipore, Billerica, MA) and 100µM β-mercaptoethanol (Sigma, St Louis, MO). N2a cells were cultured in 50% DMEM and 50% Optimem (Invitrogen) containing 10% FBS and penicillin/streptomycin. The brain-specific human V0a1 cDNA was obtained from the functional genomics facility at the University of Chicago, and cloned into expression vector pAG3 with a myc-6xHis tag at its C-terminus. WT and PSdko MEFs were stably transfected with human V0a1-myc-6xHis plasmid using Lipofectamine and plus reagent (Invitrogen), and the selected positive pool was then cultured in DMEM containing 10% FBS, penicillin/streptomycin and 200µg/ml of zeocin (Invitrogen). Human V0a1-myc-6xHis plasmid was transiently transfected into cultured WT-ES, PS1ko-ES and PSdko-ES cells using Lipofectamine 2000 (Invitrogen) and cells were harvested 48hrs after transfection for Western blot analysis. To determine transient transfection efficiencies, a plasmid encoding GFP was transfected as a control.
Experimental Animals
The generation of forebrain-specific PSEN conditional double knockout mice (PScdko mice) has been described (Mirnics et al., 2008) and 6 month old male animals were used for microarray and Western blot analysis. Transgenic mice expressing mouse prion (PrP) promoter-driven human wild type PS1, human PS1ΔE9 and human PS1M146L were described earlier (Thinakaran et al., 1996;Lee et al., 1997;Choi et al., 2008) and male animals were used for Western blot analysis. Homozygous Gt[ROSA]26Sortm4[ACTB-tdTomato,-EGFP]Luo mice (Muzumdar et al., 2007) were crossed to CamKII-Cre transgenic mouse line and 5-week old bigenic male offsprings were examined for cre-induced recombination. All experimental procedures were reviewed and approved by Institutional Animal Care and Use Committee (IACUC) of the University of Chicago (Protocol #s 71434 and 70958).
Quantitative PCR (qPCR) Analysis
Total RNA was isolated from MEF and ES cells using Trizol reagent (Invitrogen), and the isolated RNA was treated with RQ1 RNase-free DNase to remove residual DNA. First-strand cDNA was synthesized with SuperScript™ III first-strand synthesis system (Invitrogen) following the manufacturer’s instructions. The primers used for TFEB qPCR are: mouse TFEB Fw: AAGCAGCTCTGGCTCCGCAT, mouse TFEB Rv: CGGCATTTGCTCAGGCTCAG. The primers used for CatD qPCR are: mouse CatD Fw: AAGGGCAACCCGGAGGAGAA, mouse CatD Rv: TTCCACAGGCCCCACCAG AA. QPCRs of TFEB and CatD transcripts in MEF and/or ES cells were performed with RT2 SYBR® Green Fluor qPCR Mastermix (Qiagen, 330510) on an iCycler iQ™ Real-Time PCR detection system (Bio-Rad). GAPDH was used as an internal control. ΔΔCT method was used to compare the transcript level of TFEB in WT and PSdko cells.
RNA Isolation and Gene Expression Analyses
ES Cell transcriptome analysis: RNA from ES cells was processed with Single-Round RNA Amplification and Biotin Labeling System (Enzo Life Sciences) and then hybridized on a GeneChip HT MG-430 PM 24-Array Plate (Affymetrix), using two replicates for each condition. Agilent Bioanalyzer assessment reported RNA integrity numbers (RIN) of 9 or above. The data were log2 transformed using RMA. Average log2 ratios (ALRs) were calculated for each gene using the replicates. The distribution of ALRs from the autophagy and CLEAR gene groups was compared to the overall distribution of all ALRs obtained in the experiment using a groupwise, two-tailed t-test. PScdko mice transcriptome analysis: This dataset is described in detail in Mirnics et al.(2008). We used previously profiled samples from the hippocampi and frontal cortices of 6 month old PScdko and control animals. Distribution of the ALRs from the autophagy and CLEAR gene groups was assessed using the same method as described above for the ES cells.
siRNAs and Antibodies
STT3A and STT3B siRNAs and anti-STT3A or -STT3B antibodies were described previously (Ruiz-Canada et al., 2009). Anti-PSAP antibody was obtained from Dr. Shahriar Koochekpour (Hu et al., 2010). Anti-Cathepsin C (D-6, sc-74590), anti-Cathepsin D (C-20, sc-6486), anti-OST48 (E-9, sc-74408) and anti-actin (SPM161, sc-56459) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti-V0a1 polyclonal antibody was purchased from Synaptic systems (Germany). Anti-6XHis tag polyclonal antibody was purchased from Rockland Immunochemicals (Gilbertsville, PA). Anti-LC3 antibody (PM036) was purchased from MBL international (Woburn, MA). P62 antibody (P0067) was purchased from Sigma (St. Louis, MO). Polyclonal anti-PS1 NT antibody recognizes amino acids 1–65 (Kim and Sisodia, 2005), and rat anti-human PS1 antibody was a kind gift from Dr. Allan Levey (Emory University). APP antibody 369 was a kind gift from Dr. Sam Gandy (Mount Sinai School of Medicine, New York, NY).
Western Blotting
Cells and brain samples were lysed or homogenized in lysis buffer containing 50mM Tris-HCl pH7.4, 150mM NaCl, 0.5% NP40, 0.5% sodium deoxycholate, 5mM EDTA and protease inhibitor cocktail (Sigma). Protein concentrations were determined by BCA kit (Pierce, Rockford, IL). Equal amount of protein lysates were resolve on SDS-PAGE and transferred to a nitrocellulose membrane. After blocking, the membrane was sequentially incubated with primary and secondary antibodies and bound antibodies were detected by electrochemiluminescence (ECL; PerkinElmer, Waltham, MA).
STT3A and STT3B Knockdown in HEK293 Cells
HEK293 cells were repeatedly transfected with 30nM of STT3A or STT3B siRNAs with RNAiMax (Invitrogen). Human V0a1-myc-6xHis was also transfected into these cells with Lipofectamine 2000 after the initial siRNA transfection to analyze the glycosylation of V0a1 in the presence or absence of STT3A or STT3B.
PNGase F and Endo H Treatment
For PNGase F treatment, cell lysates were first supplemented with glycoprotein denaturation buffer (0.5% SDS, 40 mM DTT) and incubated at room temperature for 10mins. NP40 was then added to the mixture to a final concentration of 1%. G7 buffer (50 mM Sodium Phosphate, pH 7.5) and PNGase F (100 units) (New England Biolabs, Ipswich, MA) were then added to the mixture and incubated for 1hr at 37°C. For Endo H treatment, cell lysates were supplemented with glycoprotein denaturation buffer and incubated at room temperature for 10mins, G5 buffer (50 mM Sodium Citrate, pH 5.5) and Endo H (200 units) (New England Biolabs) were then added to the mixture and incubated for 1hr at 37°C. The PNGase F and Endo H reactions were quenched by the addition of 4X Laemmli sample buffer.
Pulse Labeling
Following transfection with siRNA and cDNA encoding human V0a1-6His, HEK293 cells were labeled for 30mins with 250µCi 35S-methionine (PerkinElmer, Waltham, MA). Cells were lysed in lysis buffer (Tris-HCl pH7.4, 150mM NaCl, 5mM EDTA, 0.5% NP40, 0.5% sodium deoxycholate and protease inhibitor cocktail) and the total radioactivity (cpm) was determined after TCA precipitation and liquid scintillation counting. Equal cpm of cell lysates was used for V0a1 immunoprecipitation. Immunoprecipitated proteins were resolved on 6% SDS-PAGE, transferred to a nitrocellulose membrane and exposed to a PhosphorImager screen. The screen was scanned using a Storm Imager (GE Healthcare, Piscataway, NJ). Imagequant 5.0 software was used for the image analysis.
Autophagy Studies
Mouse ES cells were seeded so that they reached ~90% confluency at the time of lysis and protein extraction. To induce autophagy, ES cells were starved by incubation in ES medium without FBS for 6 hours. To examine autophagic flux, ES cells were treated with bafilomycin A1 (Sigma) at 100nM for the last three hours of starvation. LC3 and p62 band intensities were quantified and normalized to actin using ImageJ software.
Fluorescent Microscopy and Image Processing
ES cells were seeded in glass bottom culture dishes (Mat Tek) so they would be ~50–70% confluent at the time of imaging (~18hrs). Cells were incubated in 1µM Lysosensor Yellow/Blue DND-160 (Invitrogen) for 5 minutes. Cells were then washed twice in PBS followed by twice in clamp buffer (5mM NaCl, 115mM KCl, 1.2mM MgSO4, 25mM MES, pH from 3.5–7.5). Cells were equilibrated in clamp buffer pH7.5 supplemented with 25µM monensin (Sigma) for 20 minutes before imaging. The same dish of cells was then washed twice with clamp buffer (pH6.0) and then allowed to equilibrate for 10 minutes before imaging. This process was repeated for all clamp buffers (pH7.5-3.5). All equilibration steps were carried out in a 37°C, 5% CO2 incubator. Imaging was performed on a heated stage (37°C). Cells were excited with attenuated UV light (357–373 nm) and observed in blue (W1; 417–483 nm) and yellow (W2; 490–530 nm) region of the spectra. Five 60X images were collected over a period of 5 minutes for each pH. 16-bit images were processed in order to subtract background fluorescence, and then ratio (W1/W2) images were generated using the image calculator function in ImageJ. Regions of interest (ROI) corresponding to vesicles were generated using W2 and applied to ratio images in order to determine the vesicles W1/W2 ratio value for each pH clamp buffer. A standard curve was generated in Prism in order to assign a predicted pH for relevant W1/W2 values. An independent standard curve was generated on each experimental day. For test groups, cells were incubated in 1µM Lysosensor Yellow/Blue DND-160 for 5 minutes, washed twice in PBS and fifteen 60X images were collected and analyzed, as above.
Immunofluorescence staining of brain sections
45µm brain coronal sections were cut on a prechilled sliding microtome block (Leica, Wtzlar, Germany), and the floating sections were immunostained with either anti-NeuN monoclonal antibody (1:800; Millipore, Temecula, CA) or anti-βIII Tubulin TUJ1 monoclonal antibody (1:500; Covance, Emeryville, CA) as described previously (Choi et al., 2008). Stained sections were visualized using DyLight-549 conjugated donkey anti-mouse antibody (1:400; Jackson ImmunoResearch, West Grove, PA) and were counter stained with DAPI (1: 15000; 4’, 6-diamidine-2’-phenylindole dihydrochloride, Roche Biochemicals, Mannheim, Germany). Both mTomato red or mEGFP expressing cells were bright enough for direct microscopic analysis.
Statistical Analysis
Data are expressed as mean values ± standard error of the mean (mean ± SEM).
Autophagy substrates are degraded efficiently in blastocyst-derived ES cell cultures that lack PSEN1 or both PSEN1 and PSEN2
Microtubule-associated protein 1 (LC3) is widely used as a marker for autophagosomes and migrates as an ~16 kDa “unprocessed” form, termed LC3-I, and an ~16 phosphatidylethanolamine-derivatized LC3-II species on SDS-PAGE. The amount of LC3 -II usually correlates well with the number of autophagosomes, or more precisely, the level of LC3-II bound to autophagic membranes (Kabeya et al., 2000). Lee et al. (2010) revealed that in nutrient-rich conditions, PS1ko blastocysts exhibited a 2-fold increase in the levels of LC3-II compared to the LC3-II levels in wild-type (WT) blastocysts. Moreover, induction of autophagy by serum withdrawal resulted in higher LC3-II levels in WT cells, but to a much lesser extent in PS1 KO cells. Finally, to assess autophagic “flux”, Lee et al. (2010) treated WT and PS1ko blastocysts with leupeptin in order to inhibit cysteine proteases in autophagolysosomes; LC3-II is degraded in autophagolysosomes and hence, LC3II turnover is thought to be a reliable measure of autophagic activity (Mizushima et al., 2010). In this case, Lee et al. (2010) showed that while leupeptin-treated WT cells showed a clear elevation of LC3II, the levels of LC3-II in PS1ko cells were unchanged over baseline. Finally, the authors examined the levels of p62/A170/SQSTM1 (hereafter termed p62) that binds to ubiquitinated proteins in the cytosol and directly interacts with LC3 that is bound to the isolation membrane/phagaphore; this LC3-p62 complex is subsequently degraded by autophagolysosomes (Ichimura et al., 2008). In this setting, Lee et al. (2010) reported that p62 levels were elevated in PS1ko cells compared to those in WT cells, suggestive of a defect in autophagic clearance. Taken together, the authors concluded that PS1ko cells had a deficit in the clearance of autophagosomal vacuoles containing LC3-II and p62.
We were intrigued by the results obtained by Lee et al. (2010) because PS1ko cells constitutively express the functionally homologous presenilin 2 (PS2) protein, and hence, we surmised that the reported effects on LC3-II and p62 levels might have been underestimated. In order to activate autophagy, we serum-starved cultured PS1ko-ES, cultured ES cells wherein PSEN1 and PSEN2 are disrupted (PSdko-ES cells) and cultured WT-ES cells for 6 hrs and assessed the levels of the autophagy substrates LC3-II and p62. In addition, we examined LC3-II or p62 levels in serum-starved cells either in the absence, or presence of bafilomycin A1, an agent that inhibits lysosomal acidification and/or inhibits autophagosome-lysosome fusion; this treatment leads to reduced degradation and hence, accumulation of autophagy substrates (Tanida et al., 2005).
Much to our surprise, and in striking contrast to the results obtained by Lee et al. (2010), upon serum starvation, we observed a significant reduction in LC3-II levels in cultured PS1ko-ES cells compared to cultured WT-ES cells, and this level was reduced further in cultured PSdko-ES cells (Fig. 1A, compare lanes 1, 3 and 5, respectively). Our demonstration of lower LC3-II levels in cultured PS1ko-ES and PSdko-ES cells could be interpreted to suggest that autophagosome membranes are limiting, or that LC3-II is rapidly degraded in autophagolysosomes following fusion of autophagosomes with lysosomes. To address this issue, we assessed “autophagic flux”, a measure of autophagic activity (Mizushima et al., 2010). To examine autophagic flux, we treated serum starved cells with bafilomycin; the differences in the amount of LC3-II between samples in the presence and absence of bafilomycin A1 represent the amount of LC3 that is delivered to lysosomes for degradation (Klionsky et al., 2008;Mizushima and Yoshimori, 2007;Rubinsztein et al., 2009). In this case, we observed that LC3-II levels were elevated in cultured WT-ES cells (Fig. 1A, compare lanes 1, 2) as expected, but even more remarkably, the levels of LC3-II were substantially elevated in the cultured PS1ko-ES and PS1dko-ES cells compared to the levels observed in respective cells maintained in starvation conditions (Fig. 1A, compare lanes 3, 4 and 5, 6, respectively; quantified in 1B and 1C). Hence, these studies reveal that the reduced steady-state level of LC3-II in serum-starved cultured PS1ko-ES and PSdko-ES cells is the result of elevated autophagolysosomal activity in these cells compared to cultured WT-ES cells, thus arguing that autophagosomal activity in cultured PS1ko-ES and PSdko-ES cells is unimpaired.
Figure 1
Figure 1
Quantification of LC3 and p62 Levels in the presence or absence of bafilomycin A1 in cultured WT-ES, PS1ko-ES and PSdko-ES Cells. A, LC3 levels detected by Western blotting. B, Quantification of LC3 II levels normalized to actin (*p<0.06, **p<0.01, (more ...)
To validate these latter conclusions, we examined the turnover of p62, in serum starved cultured WT-ES, PS1ko-ES and PSdko-ES cells either in the absence, or presence of bafilomycin. Under these conditions, we observed that compared to serum starved cultured WT-ES cells, the steady-state levels of p62 were slightly elevated in serum starved cultured PS1ko-ES and PSdko-ES cells (Fig. 1D, compare lanes 1, 3, 5, respectively), as was previously reported (Lee et al., 2010). While the elevation in levels of p62 in the cultured PS1ko-ES and PSdko-ES cells may be indicative of autophagy failure, as was claimed earlier (Lee et al., 2010), it was necessary to confirm this proposal using the autophagic flux assay. In this regard, treatment of starved cells with bafilomycin revealed a clear elevation in p62 levels in all cell lines analyzed (Fig. 1D, compare lanes 1 and 2, 3 and 4, 5 and 6 respectively; quantified in 1E and 1F). We confirmed the lack of expression of PS1 in the PS1ko-ES and PSdko-ES cells by Western blot analyses (Fig. 1G, lanes 3–6). In parallel, and due to very low levels of PS2 detected with our antibodies in ES cell lysates, we chose to employ RT-PCR to confirm the lack of PSEN2 mRNA in PSdko-ES cells (Fig. 1H, lane 4; compare to lanes 2, 3).
Collectively, our autophagic flux analyses of p62 and LC3-II lead us to conclude that autophagy is as active in the cultured PS1ko-ES and PSdko-ES cells.
Mean vesicle pH in cultured WT-ES and PS1ko-ES Cells are equivalent, but is slightly more acidic in cultured PSdko-ES cells
In an attempt to demonstrate that the defects in autophagy seen in PS1ko blastocysts were the result of alterations in lysosomal pH, Lee et al. (2010) used a LysoSensor yellow/blue DND-160–Dextran probe to reveal that WT cells displayed an average lysosomal pH of 4.7±0.08, while PS1ko blastocysts displayed a substantially elevated lysosomal pH of 5.4±0.04. We attempted to reproduce the findings of Lee et al. (2010) using an unconjugated pH-dependent fluorescent probe, LysoSensor DND-160 that interrogates the pH of all intracellular organelles, and is not specific to lysosomes. We generated a standard curve by obtaining images of cultured WT-ES cells, at both blue (W1) and yellow (W2) wavelengths, that were incubated in MES buffer clamped at various pH values (pH7.5-3.5). The pH-sensitive nature of the dual emission probe is clearly seen in Figure 2A, and the W1/W2 ratios (Fig. 2B) allowed us to generate a standard curve (Fig. 2C). We then incubated cultured WT-ES, PS1ko-ES and PSdko-ES cells with the Lysosensor-DND-160 dye and obtained pseudo colored images (Fig. 2D) from which we quantified the W1/W2 ratio distribution of a population of vesicles in each cell line (Fig. 2E). Converting the W1/W2 ratio to vesicle pH revealed that the mean vesicle pH for cultured WT-ES and PS1ko-ES cells (Fig. 2G) are not significantly different, but that there is significant increase in the population of vesicles with lower pH values in cultured PSdko-ES cells (Fig. 2F). While the mechanism(s) by which PS-deficiency decreases overall intracellular vesicle pH in cultured PSdko-ES cells have yet to be determined, our studies in cultured PS1ko-ES cells fail to confirm the results of Lee et al. (2010) that showed an acidification defects in PS1ko blastocysts.
Figure 2
Figure 2
Mean vesicle pH measurement in cultured WT-ES, PS1ko-ES and PSdko-ES Cells. A, Representative images of cultured WT-ES cells used for ratiometric analysis using the pH sensitive probe Lysosensor-DND-160. Images were collected while ES cells were incubated (more ...)
Processing of cathepsin D is not impaired in cultured PS1ko-ES and PSdko-ES cells or in brains of mice lacking PS in excitatory neurons
In view of the reported deficits in lysosomal acidification, Lee et al. (2010) examined the proteolytic maturation of the lysosomal hydrolase, cathepsin D (CatD). It is well-established that pro-CatD is transported from the Golgi complex to acidic endolysosomal compartments where the propeptide is removed to generate an active ~48 kDa active intermediate that is further processed into an ~14 kDa amino terminal light chain and an ~34 kDa carboxyl-terminal heavy chain (Zaidi et al., 2008). Western blot studies by Lee et al. (2010) revealed that in PS1ko blastocysts, the steady-state ~48 kDa intermediate accumulated to high levels, while the ~34 kDa and ~14 kDa chains of CatD were significantly reduced compared to those in WT blastocysts, wherein the levels of the processed derivatives were prominent. These findings lead the authors to conclude that lysosome-specific functions necessary for CatD processing were impaired in PS1ko cells. In view of our failure to demonstrate any alteration in lysosomal pH in cultured PS1ko-ES and PSdko-ES cells, we reexamined CatD processing in these cells and in cultured WT-ES cells. In contrast to the results reported by Lee et al. (2010), we now show that the steady-state levels of either the ~48 kDa intermediate form or the ~34 kDa heavy chain of CatD in detergent lysates of cultured WT-ES, PS1ko-ES and PSdko-ES cells are indistinguishable (Fig. 3A, lanes 1–3).
Figure 3
Figure 3
Processing of cathepsin D in the absence of presenilin(s). A, Processing of cathepsin D in cultured WT-ES, PS1ko-ES and PSdko-ES cells. Intermediate and heavy chain of CatD are shown in the upper two panels respectively; actin is used as an internal loading (more ...)
It should be noted that the steady-state levels of the ~52 pro-CatD and ~48 kDa intermediate forms of CatD in lysates from cultured PS1ko-ES and PSdko-ES cells are elevated compared to the levels of these CatD derivatives in cultured WT-ES cells (Fig. 3A). Our earlier microarray studies of the hippocampus and cortex from PScdko mouse brain (see below) revealed an age-dependent elevation in mRNA encoding CatD that was accompanied by an increase in the steady-state levels of the ~48 and ~34 kDa CatD-related polypeptides (Mirnics et al., 2008). To assess the possibility that the elevated levels of CatD protein in the cultured PS1ko-ES and PSdko-ES cells were a reflection of changes in CatD mRNA, we prepared total RNA from cultured WT-ES, PS1ko-ES and PSdko-ES cells and quantified the steady-state levels of mRNA encoding CatD. We failed to observe any differences in transcripts encoding CatD between the cultured ES cells (Fig. 3B, p>0.05). Supporting this conclusion, transcriptomic analysis of WT-ES cells, PS1ko and PSdko ES cells failed to reveal an elevation of CatD transcripts in the PS1ko and PSdko ES cells (ctsd; Table 2). Hence, the mechanism(s) that regulates the apparent increase in steady-state levels of CatD in PS1ko-ES and PSdko-ES cells remains to be determined.
Table 2
Table 2
Differential expression of CLEAR network genes in PSdko, PS1ko and WT ES cells
Finally, we assessed CatD processing in the brains of PS1lox/lox/PS2−/−/cre mice (hereafter referred to as PScdko mice). To generate the PScdko mice, mice in which endogenous PSEN1 with internal loxP sites (PS1lox/lox) were crossed with PSEN2-deficient (PS2−/−) mice. Mice homozygous at both loci were crossed with mice expressing cre recombinase driven by the α-calmodulin kinase II gene promoter (α-CamKII-cre) that is only active postnatally in excitatory neurons, and subsequently backcrossed to generate mice that lack expression of PSEN1 in excitatory neurons and PSEN2 throughout the neuraxis and in peripheral tissues. Earlier studies have reported that these PScdko mice exhibit age-associated neurodegeneration throughout the forebrain after the age of 5–6 months (Saura et al., 2004;Chen et al., 2008). Indeed, Lee et al. (2010) used ultrastructural approaches to reveal that the brains of these animals exhibited abnormally elevated levels of LC3-positive vesicles, and decreased acidification of lysosomal compartments, findings that were interpreted to suggest that PSEN-deficiency impairs autophagolysosomal function in an in vivo setting. We now show that while the steady-state levels of CatD are elevated in PScdko hippocampus, as previously reported (Mirnics et al., 2008), the patterns of CatD-related polypeptides in lysates from the hippocampi of PScdko mice and control littermates are indistinguishable (Fig. 3C, lanes 1 and 2). We confirmed that PS1 expression was largely eliminated in brains of PScdko mice (Fig. 3C, lane 2), leading to the hyperaccumulation of APP-CTFs (Fig. 3C, lane 2).
Glycosylation of the V0a1 subunit of V-ATPase is not regulated by PS
In view of the acidification defects observed in PS1ko blastocysts, Lee et al. (2010) examined the subcellular distribution and glycosylation of the V0a1 subunit of the vacuolar ATPase (V-ATPase) that is essential for proton translocation and lysosomal acidification. The authors reported that in contrast to WT cells, V0a1 was primarily localized to the endoplasmic reticulum and depleted from lysosomal compartments in PS1ko cells. Consistent with these findings, Western blot analysis revealed that while WT cells expressed both ~100 kDa immature/unglycosylated species and endo H-resistant ~120 kDa V0a1-related species, V0a1 failed to mature in PS1ko cells, resulting in the accumulation of endo H-insensitive ~100 kDa species.
Our initial attempts to detect V0a1 with a commercially-available antibody disclosed extremely low, albeit detectable, levels of this subunit in lysates of MEF or ES cells (data not shown). Thus, we generated a brain-specific human V0a1 cDNA (Nishi and Forgac, 2000) with a myc-6xHis epitope tag at its C-terminus (V0a1-6His), and examined the maturation of the encoded polypeptide in transfected MEF or ES cells. We stably expressed V0a1-6His in cultured WT or PSdko MEF cells and examined the glycosylation status of the ectopically-expressed V0a1-6His. Surprisingly, we observed that the pattern and levels of ~110 kDa and ~120–140 kDa human V0a1-6His-related polypeptides in cultured WT and PSdko MEF cells were indistinguishable (Fig. 4A, compare lanes 1 and 4) and in both cases, the ~120–140 kDa species were equally susceptible to digestion with PNGase F, an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides, resulting in the generation of an ~100 kDa species (Fig. 4A lanes 2 and 5). The fact that the ~120–140 kDa V0a1-6His species remained resistant to digestion with endo H, an endoglycosidase which cleaves asparagine-linked mannose-rich oligosaccharides, but not highly processed complex oligosaccharides (Fig. 4A, compare lanes 3 and 6), suggests that V0a1-6His polypeptides have acquired hybrid and/or complex oligosaccharides in WT MEF cells and MEF cells that lack both PSEN1 and PSEN2.
Figure 4
Figure 4
V0a1 is glycosylated in the absence of presenilin(s). A, Stably expressed human V0a1-6His is glycosylated in cultured PSdko MEF. The treatment of the lysates with PNGase F or Endo H is indicated above lanes 2, 3, 5 and 6. The respective presence and absence (more ...)
To validate these findings in ES cells, we transiently transfected cDNA encoding human V0a1-6His into cultured WT-ES, PS1ko-ES and PSdko-ES cells. Although the levels of accumulated V0a1 in each cell line varied, this was due to differences in transient transfection efficiencies; using a GFP reporter assay, we estimated the transient transfection efficiencies of WT-ES, PS1ko-ES and PSdko-ES cells to be between 30–50%, 20–30% and 10–20%, respectively. Western blot analysis revealed that as in cultured WT-ES cells, transiently expressed human V0a1-6His in cultured PS1ko-ES and PSdko-ES cells acquired mixed and/or complex oligosaccharides (Fig. 4B, lanes 1, 3, 5). Collectively, our studies in both MEFs and ES cells reveal that the absence of PSEN1 expression either singly, or in combination with PSEN2, has no impact on oligosaccharide maturation of V0a1, findings that fail to confirm the findings of Lee et al. (2010).
Finally, we examined the glycosylation of endogenous V0a1 in brains of PScdko mice. We now show that in PScdko (PS1lox/lox/PS2−/−/Cre) mice, mouse V0a1 acquires hybrid and/or complex oligosaccharides (Fig. 4C, lanes 4–6), compared to littermate control PS1lox/lox/PS2−/− mice in which α–CamKII-cre is not expressed, but in which only the PSEN2 gene is disrupted (Fig. 4C, lanes 1–3). It should be noted that in PScdko mice, α–CamKII-cre-mediated disruption of endogenous PSEN1 is incomplete (Fig. 3C, compare PS1 NTF in lanes 1 and 2); our estimate is that <10% of PS1 remains after PSEN1 disruption specifically in excitatory neurons (for quantification, see Figure 6A, compare lanes 11 and 14). Thus, it might be argued that this residual level may have been sufficient to support maturation of V0a1 in PScdko mice, despite the fact that the levels of full-length PS1 are essentially undetectable (see Figure 6A, below). Moreover, we have shown that in the PScdko mice at 6 months of age, PSEN1 is fully deleted in neurons within the CA fields and granule cells within the hippocampus (Feng et al., 2001). In this regard, it is well-established that PSEN1 expression is not restricted to excitatory neurons (Lee et al., 1996;Sherrington et al., 1995;Lah et al., 1997), and hence, we would suggest that the residual PS1 signal observed in these studies is a reflection of expression in interneurons and nonneuronal cells. More importantly, and in view of the fact that the total levels of V0a1 in WT and PScdko mice are comparable, we find it inconceivable that the levels of mature V0a1 in the PScdko mice represent expression in those cells wherein PSEN1 remains intact.
Figure 6
Figure 6
Full-length PS1 does not coimmunoprecipitate with V0a1 or STT3B. A, A series of diluted lysates of cultured WT MEF and WT-ES cells or mouse hippocampi are used to assess the levels of full length PS1 and PS1 NTF. Very low level of full length PS1 is detected (more ...)
Analysis of V0a1 maturation and CatD processing in transgenic mice and neuroblastoma N2a cells expressing WT and FAD-linked PS1 variants
Lee et al. (2010) extended their studies of PS1ko blastocysts to examine the impact of expressing FAD-linked PS1 variants (PS1-FAD) on autophagy. The authors reported that degradation of long-lived proteins in several of PS1-FAD fibroblast cell lines that express independent PS1 variants was lower compared to that in control cells, and that in one FAD-PS1 fibroblast line, both the LC3-II/LC3-I ratio and total LC3-II levels were higher than that observed in control fibroblasts. Furthermore, LysoTracker staining revealed that lysosomal pH in one PS1-FAD fibroblast line was substantially higher than control fibroblasts. In order to assess the possibility that the acidification deficits could be attributed to defective maturation of V0a1, the authors reported that in contrast to control fibroblasts in which V0a1 colocalized with CatD-positive compartments, very little V0a1 colocalized with CatD in PS1 FAD fibroblasts, but instead colocalized with an ER-resident protein, protein disulfide isomerase (PDI). Additional biochemical studies on a single FAD-PS1 fibroblast line showed decreased levels of maturation of V0a1 compared to that in control fibroblasts. Thus, the authors concluded that “cells expressing FAD-linked PS1 display a similar loss of lysosomal function as in PS1ko cells that might account for the marked acceleration of autophagy-related dysfunction and neuronal cell death associated with PS1-FAD”.
In an attempt to validate these findings, we examined V0a1 maturation and CatD processing in stable N2a cell lines that express independent FAD-linked PS1 variants and in brains of transgenic mice that express either human WT PS1 or two FAD-linked variants in a ubiquitous manner. For the former, we transiently transfected stable N2a “pools” expressing either human PS1, or the FAD-linked A280E or ΔE9 variants with cDNA encoding human V0a1-6His (see above), and examined the expression and maturation of the ectopically-expressed protein (Fig. 5A). In contrast to the report by Lee et al. (2010) that revealed a defect in maturation of V0a1 in a single FAD-PS1 fibroblast line, we failed to demonstrate that oligosaccharide maturation of the expressed V0a1 in N2a cells that express either the A280E or ΔE9 variants (Fig. 5A, lanes 7 and 8; 9 and 10, respectively) is any different from that observed in cells expressing WT PS1 (Fig. 5A, lanes 5 and 6). Extending these findings, we assessed CatD maturation in the stable N2a cell “pools” as a surrogate marker for lysosomal acidification and function. Western blot analysis revealed that the steady-state levels of the intermediate and heavy chains of CatD were identical between cells that express either PS1 or the FAD-linked A280E and PS1ΔE9 PS1 variants (Fig. 5B, compare lanes 2, 3, 4, respectively). Finally, we used PS1NT antibody to confirm stable expression of PS1 and FAD-linked variants in the stable N2a cell “pools” (Fig. 5B, lower panel). These studies reveal that endogenous PS1 (arrowhead; Fig. 5B, lane 1) is subject to “replacement” by exogenous human PS1 (Fig. 5B, lanes 2–4) as a result of competition for limiting, endogenous components of the γ-secretase complex (nicastrin, Aph-1 and PEN-2), as we described earlier (Thinakaran et al., 1996;Thinakaran et al., 1997). Notably, while full-length human PS1 is apparent in the WT and A280E “pools”, these species are known to be rapidly degraded (Thinakaran et al., 1997;Ratovitski et al., 1997). The effect on “replacement” is most obvious for cells expressing the PS1ΔE9 variant (Fig. 5B, lane 4) that fails to undergo endoproteolytic processing due to deletion of a region that contains the endoproteolysis site, but nevertheless competes for limiting factors in the γ-secretase complex (Thinakaran et al., 1996;Lee et al., 1997).
Figure 5
Figure 5
V0a1 is glycosylated and cathepsin D is processed into mature forms in cells and brains of mice expressing independent FAD-linked mutant PS1. A, Human V0a1-6His is glycosylated in N2a cells stably expressing FAD-linked PS1 mutants E280A and ΔE9. (more ...)
Finally, we examined V0a1 maturation in the brains of mice that express mouse prion promoter-driven transgenes encoding either human WT PS1 (Thinakaran et al., 1996) or the FAD-linked M146L or ΔE9 variants (Lee et al., 1997). These studies document that the maturation of V0a1 in brains of mice that express either human PS1 or FAD-linked PS1 polypeptides in a ubiquitous manner is indistinguishable (Fig. 5C, compare lanes 3, 4 to lanes 5, 6 and 7, 8). Extending these experiments, we examined the maturation of CatD in the brains of our transgenic mouse lines. As we observed in the N2a cell lines, the levels of CatD-related intermediate and heavy chains were indistinguishable in brain extracts from mice expressing either human PS1 or the FAD-linked M146L or ΔE9 variants (Fig. 5D, compare lanes 2–4, respectively). Finally, we confirmed the expression of PS1 and FAD-linked variants in the transgenic mouse lines and the “replacement” of endogenous PS1 (Fig. 5D lower panel, lanes 2–4), as we had previously documented (Thinakaran et al., 1997). Collectively, our studies reveal that expression of FAD-linked PS1 variants either in stable cell lines or in the brains of transgenic mice neither impairs the glycosylation of V0a1, nor the maturation of CatD and hence, fail to support the conclusions by Lee et al. (2010) that cells expressing FAD-linked PS1 exhibit a loss of lysosomal function.
Mechanistic analysis of PS1-mediated regulation of V0a1 maturation
Using coimmunoprecipitation approaches, Lee et al. (2010) reported that full-length PS1 associated both with the immature, unglycosylated form of V0a1 and STT3B, a subunit of the oligosaccharyltransferase (OST) complex that is involved in posttranslational glycosylation of selected substrates. From these findings, the authors proposed that full-length PS1 serves as a chaperone to assist in posttranslational glycosylation of V0a1; in the absence of PS1, V0a1 fails to acquire oligosaccharide modifications, and thus, does not exit from the endoplasmic reticulum and is subsequently degraded. As a consequence of failed maturation and targeting of V0a1 to lysosomal compartments, lysosomal pH is elevated, leading to impaired autophagosomal function. We were perplexed by these findings because we (Thinakaran et al., 1996;Thinakaran et al., 1997;Ratovitski et al., 1997;Lee et al., 1996) and others (Podlisny et al., 1997) have reported that in untransfected cells, murine tissues and human and monkey brain, PS1 and PS2 accumulate as ~30 kDa amino- (NTF) and ~20 kDa carboxyl-terminal (CTF) derivatives at steady-state and that full-length PS1 and PS2 are virtually undetectable. Confirming these latter findings, we performed western blots of detergent-solubilized extracts from cultured WT and PSdko MEF (Fig. 6A, lanes 1–5), cultured WT-ES and PSdko-ES cells (Fig. 6A, lanes 6–10) or from hippocampi from PS1lox/lox/PS2−/− and PScdko mice (Fig. 6A, lanes 11–14) using anti-PS1NT antibody raised against the intracellular amino-terminal domain of PS1. We show that full-length PS1 is detectable, albeit at extremely low levels in lysates prepared from cultured WT MEF and WT-ES cells, but is virtually absent in extracts prepared from mouse hippocampus.
In view of the inherent difficulty of detecting full-length PS1 in mammalian cells or in brain, we used two alternative approaches to test the model that full-length PS1 interacts with V0a1 and STT3B. In the first, we stably expressed V0a1-6His in a HEK293 cell line that constitutively expresses a short hairpin (sh) duplex designed to target mRNA encoding PEN-2 (Prokop et al., 2004). In the PEN-2 knockdown (PEN2KD) line, the level of PEN-2, a subunit of the γ-secretase complex that is essential for PS endoproteolysis and “activation” of the enzyme (Ahn et al., 2010), is markedly reduced (Fig. 6B, compare lanes 1 and 2), leading to accumulation of full-length PS1 (Fig. 6B, lane 2). Using anti-6xHis antibodies to immunoprecipitate exogenous V0a1 and associated proteins, we failed to recover full-length PS1 or PS1-NTFs (Fig. 6C, lane 3). Similarly, α-PS1NT failed to coimmunoprecipitate V0a1-6His (Fig. 6C, lane 3’), under conditions in which this antibody successfully recovered endogenous NCT, a subunit of the γ-secretase complex (Fig. 6C, lane 3”). We then asked whether full-length PS1 interacts with the OST subunit, STT3B. Using anti-STT3B antibodies, we failed to recover full-length PS1 or PS1-NTFs (Fig. 6D, upper panel lane 3), under conditions in which the antibody successfully coimmunoprecipitated OST48, an established subunit of the OST enzyme complex (Fig. 6D, lower panel, lane 3). Reverse-coimmunoprecipitation studies using anti-PS1NT antibody failed to coimmunoprecipitate STT3B (Fig. 6D, lane 3’), under conditions wherein this antibody recovered both full-length PS1 and PS1 derivatives (Fig. 6D, lanes 3’). Collectively, our studies fail to demonstrate an association of full-length PS1 with either V0a1 or STT3B.
While these latter studies failed to reveal an interaction of STT3B or V0a1 with full-length PS1 in PEN2KD cells, it was conceivable that in these cells, the association of full-length PS1 with nicastrin and APH-1 in the “pre-activation” complex (Takasugi et al., 2003) might hinder potential interactions with STT3B or V0a1. To address this possibility, our second strategy was to employ a stable N2a cell line that overexpresses human WT PS1 (Borchelt et al., 1996). In these cells, Western blot analysis of detergent lysates reveals that full-length PS1 is detectable, but is relatively unstable (Ratovitski et al, 1997). We now show that in N2aWT PS1 cells, full-length PS1 is not recovered with either anti-STT3B, or anti-V0a1 antibodies (Fig. 6E, lanes 5, 6) to levels any higher than found using normal IgG (Fig. 6E, lane 4), while anti-PS1 antibodies efficiently immunoprecipitate both full-length PS1 and PS1 NTF (Fig. 6E, lane 7). Hence, in two independent settings, namely, PEN2KD cells and cells overexpressing PS1, we fail to document the association of PS1 with V0a1 or STT3B.
A model proposed by Lee et al. (2010) is that full-length PS1 regulates the maturation of V0a1 by holding it in proximity to STT3B, and thereby facilitates posttranslational glycosylation of the substrate. To examine the role of STT3B in glycosylation of V0a1, we transfected HEK293 cells with V0a1-6His and treated these cells with siRNAs that selectively target the two principal OST subunits that are critical for cotranslational (STT3A) or posttranslational glycosylation (STT3B) of glycoproteins (Ruiz-Canada et al., 2009).
We find that treatment with siRNA specific to each subunit selectively and effectively blocks synthesis of the respective target proteins (Fig. 7A, panel STT3A lanes 4, 5; panel STT3B lane 6, 7). More importantly, we observe that the maturation of prosaposin (PSAP), a polypeptide that is selectively glycosylated by STT3A, is significantly impaired in cells lacking STT3A (Fig. 7A, panel PSAP, lanes 4, 5). Similarly, the maturation of procathepsin C (proCatC), a STT3B-specific substrate, is also diminished in cells lacking STT3B (Fig. 7A, Panel pCatC, lanes 5, 6). Notably, procathepsin C is difficult to detect by Western blotting because proCatC, like CatD, is proteolytically processed in the lysosome. Interestingly, we failed to detect any effects on the maturation of V0a1-6His by reducing the levels of either STT3A or STT3B in these cells (Fig. 7A, Panel V0a1, lanes 4–7). We then considered the possibility that V0a1 might be long-lived, hence masking the effects of lowering STT3A or STT3B on newly-synthesized V0a1. Hence, we chose to perform pulse-labeling and immunoprecipitation studies to examine the levels and glycosylation of newly-synthesized V0a1-6His. In this case, we observe that siRNA-mediated reduction of STT3B levels has very little impact on the maturation of V0a1-6His, but surprisingly, reduction of STT3A levels leads to a profound and reproducible elevation in the mature forms of V0a1-6His. It is not presently clear if this is the result of an elevation in STT3B levels in cells in which STT3A is diminished (Fig. 7B, panel STT3B, lanes 3, 4). However, we previously showed that depletion of STT3A does not fully eliminate cotranslational glycosylation, a finding that led us to conclude that the STT3B isoform has cotranslational access to skipped glycosylation sites (Ruiz-Canada et al., 2009), and hence, it is conceivable that the increased level of newly-synthesized V0a1-6His might be the result of STT3B-dependent glycosylation of V0a1 that would otherwise be modified by STT3A. Indeed, analysis of single knockdowns of STT3A or STT3B revealed that certain glycoproteins like ribophorin I were not hypoglycosylated, and that three of the four glycosylation sites in proCatC can be cotranslationally modified by either STT3A or STT3B. These data, taken together with our results indicate that the glycosylation site in V0a1 can be glycosylated by both the STT3A and STT3B isoforms of the OST.
Figure 7
Figure 7
V0a1 is glycosylated when either STT3A or STT3B is downregulated in HEK 293 Cells. A, HEK 293 cells were transfected with control siRNA (lanes 1 to 3), STT3A siRNA (lanes 4 and 5) or STT3B siRNA (lanes 6 and 7) followed by a transient transfection with (more ...)
Expression of genes associated with autophagy is not altered in cultured PS1ko-ES and PSdko-ES cells and brains of adult mice that lack PS in excitatory neurons
Our studies have revealed that the absence of expression of PSEN1 alone, or in combination with PSEN2, does not influence the autophagolysosomal turnover of LC3-II or p62. In order to gain a more global assessment of autophagic activity, we chose to examine the transcription of genes associated with autophagy using two approaches. First, we asked whether the expression of a recently described master transcriptional regulator of lysosomal biogenesis and autophagy, termed TFEB (Settembre et al., 2011), is altered in cultured PSdko MEF or ES cells compared to corresponding WT cells. The rationale for this study is that transient overexpression or siRNA-mediated suppression of TFEB level leads to elevated or suppressed expression of TFEB target genes, respectively (Settembre et al., 2011). Real-time PCR analysis failed to reveal a statistically significant difference in levels of transcripts encoding TFEB in cultured PSdko MEFs or ES cells compared to WT counterparts (Fig. 8A; ΔΔCT = −0.1, p>0.2), findings which strongly argued against a defect in the expression of genes that are controlled by this transcriptional regulator of the autophagic pathway in PSEN-deficient cells. In order to validate these findings, we then assessed transcriptome profiles in cultured WT-ES, PS1ko-ES and PSdko-ES cells using high density oligonucleotide arrays, paying particular attention to the expression of a set of genes that have been recently reported (Settembre et al., 2011) to be direct targets of TFEB, a transcription factor that binds a consensus palindromic 10–base pair (bp) GTCACGTGAC motif, as well as genes that belong to autophagy machinery components, including UVRAG, WIPI, MAPLC3B, SQSTM1, VPS11, VPS18, ATG9B, AMBRA1, ATG12, ATG16L1, ATG4A, ATG4B, ATG4C, ATG4D, ATG5, ATG9A, BECN1, GABARAP, GABARAPL1, GABARAPL2, IRGM, MAP1LC3A, RGS19, ULK1. As a gene group, these transcripts showed no significant enrichment compared to overall distribution of transcripts (mean ALRPSdko-CNT =−0.13; p=0.267) (Table 1), and only the mRNA encoding ATG9B showed a marked increase in expression in the cultured PS1ko-ES and PSdko-ES cells compared to WT-ES cells (218% and 65%, respectively). Thus, with the exception of ATG9B, there does not appear to be a general alteration in expression of TFEB-target autophagy machinery components. In parallel to the analysis of TFEB target genes that are involved in autophagy and autophagy machinery component genes, we assessed TFEB target genes that are critical for lysosomal biogenesis. These genes belong to the Coordinated Lysosomal Expression and Regulation (CLEAR) network (Sardiello et al., 2009). Compared to the overall distribution of average log ratios between the cultured PSdko-ES and WT-ES cells, TFEB target genes that are critical for lysosomal biogenesis showed a populational shift that was significant (mean ALRPSdko-CNT=−0.24; p=0.00013). Of these, we observed a greater than two-fold down-regulation of ACP5, CTSF, CTSH, GGH, and LIPA transcripts (Table 2). This suggested that compound deficiency of PSEN1 and PSEN2 is affecting the expression of genes related to lysosomal biogenesis, and hence, we sought to confirm this hypothesis in an in vivo model.
Figure 8
Figure 8
Expression of genes associated with autophagy and lysosomal biogenesis in cells and mouse brain. A, Quantitative PCR analysis of TFEB expression in WT and PSdko MEF and ES cells. The results represent the mean± SEM of four sets of reactions for (more ...)
Table 1
Table 1
Differential expression of autophagy genes in PSdko, PS1ko and WT ES cells
To this end, we examined the transcriptome profiles in the frontal cortex and hippocampi of PScdko mice. We first confirmed the selective expression of cre recombinase in mature excitatory neurons by crossing the α-CamKII-Cre transgenic mice (line T29-1; Tsien et al., 1996) with Gt[ROSA]26Sortm4[ACTB-tdTomato,-EGFP]Luo mice (Muzumdar et al., 2007), that harbor a knockin cassette within the mouse ROSA26 locus. The knockin cassette contains loxP sites on either side of sequences encoding a membrane-targeted tdTomato [mTomato red] cassette upstream of sequences encoding a membrane-targeted EGFP [mEGFP] (Fig. 8B). Constitutive expression of mTomato red results in strong red fluorescence in all brain regions and cell types (Fig. 8B). When bred to α-CamKII-Cre mice, the resulting offsprings have the mTomato red cassette deleted in the cells expressing cre recombinase, thus activating expression of mEGFP (Fig. 8B). Examination of 5 week old bigenic mice carrying both α–CamKII-Cre and ROSA26 reporter transgenes revealed the expression of mEGFP, a direct read out of cre recombinase activity, throughout the cortex and hippocampus (Fig. 8Ci). In contrast, the single transgenic Gt[ROSA]26Sortm4[ACTB-tdTomato,-EGFP]Luo mice brain sections showed uniform mTomato red expression (Fig. 8Cii), with no detectable mEGFP expression (Fig. 8Ciii). In comparison to the single transgenic Gt[ROSA]26Sortm4[ACTB-tdTomato,-EGFP]Luo line, no gross changes in anatomical regions of the brain were observed in bigenic mice. Furthermore, confocal imaging revealed that the morphology of mEGFP positive cells in bigenic mice resemble mature granule cell neurons of the dentate gyrus (Fig. 8Civ), pyramidal cell neurons in CA1 (Fig. 8Cviii), mossy fibers in CA3 (Fig. 8Cxii), and neurons in all layers of cortex (Fig. 8Cxvi). Importantly, no detectable cre-induced mEGFP expression is observed in non-neuronal cell types, including vascular endothelium that was positive for mTomato red (Fig. 8Cv, ix, xiii and xvii). These data confirm that cre recombinase activity in the bigenic mice is specific to postmitotic, excitatory neurons, without activity in other CNS cell types.
Finally, in view of studies which have reported that cre recombinase can promote recombination at cryptic sites(Semprini et al., 2007;Thyagarajan et al., 2000) and that high levels of cre expression driven by a nestin promoter in neuronal progenitors causes defects in proliferation, aneuploidy and cell death(Forni et al., 2006), we compared the neuronal cell morphology and nuclear architecture in WT mice and in mice that express CamKII promoter-driven cre recombinase. We observed neither cellular nor nuclear alterations in neurons in the hippocampus or cortex of mice expressing CamKII-cre (Fig. 8D).
In our earlier efforts, we reported that mice with compound deficiencies of PSEN1 and PSEN2 in excitatory neurons lead to a progressive, age-dependent transcriptome signature related to neurodegeneration and neuroinflammation (Mirnics et al., 2008). Reexamination of this dataset failed to uncover a change in the expression of either TFEB, or any TFEB target genes associated with autophagy or other autophagy-related genes (Table 3). However, there was a clear and highly significant elevation in the expression of the lysosomal biogenesis “CLEAR” network genes in both the fontal cortex (FC) and hippocampus (HC) of 6 month old PScdko mice compared to littermate PS1lox/lox/PS2−/− mice controls (Fig. 8E and 8F) (FC: p=2.0*10−19; HC: p=6.6*10−9). The most overexpressed genes in the lysosomal biogenesis network in both the FC and HC included: CTSD, NAGLU, GUSB, CTSZ, CTSS, HEXB and CD68.
Table 3
Table 3
Differential expression of TFEB-dependent autophagy and lysosomal biogenesis (CLEAR) genes in the brain of 6 month old PSdko mice
Thus, in both cultured PS1ko-ES and PSdko-ES cells and brains of PScdko mice, we failed to observe significant alterations in the expression of TFEB target genes involved in autophagy. However, in brains of PScdko mice, we found evidence of altered expression of the genes involved in lysosomal biogenesis, defined as the “CLEAR” network (Sardiello et al., 2009).
The accumulation of autophagic vacuoles is a consistent feature of many neurodegenerative diseases (Shintani and Klionsky, 2004). The significance of the described perturbations in the autophagic pathways in Alzheimer’s disease gained support by a report by Lee et al. (2010), in which the authors concluded that the absence of PSEN1 expression impaired the clearance of autophagosomes during macroautophagy due to a selective impairment in lysosomal acidification. These deficits were attributed to failed association of PS1 holoprotein with STT3B and V0a1, and defects in posttranslational glycosylation and targeting of the V0a1 to lysosomes. Moreover, human fibroblasts expressing FAD-linked PS1 variants also exhibited lysosomal/autophagy deficits similar to cells lacking PS1.
In view of the provocative outcomes reported by Lee et al. (2010), we reexamined several aspects of the model and now offer several insights that collectively, fail to support the conclusions drawn by the authors. First, we document that while LC3-II levels are considerably diminished in cultured PSdko-ES cells compared to WT-ES cells, autophagic flux assays reveal that this is the result of an active autophagic pathway in cells lacking PSEN.
Second, we demonstrate that the mean vesicle pH in cultured PS1ko-ES cells and WT-ES cells are essentially identical, but the vesicle pH in PSdko-ES cells exhibits a shift towards more acidic pH values. The mechanism(s) underlying this observation remains to be determined, but Neely et al. (2011) recently showed that vesicle acidification was unchanged in MEFs lacking PSEN compared to WT MEFs. Third, we show that proteolytic processing of CatD is indistinguishable in cultured WT-ES, PS1ko-ES and PSdko-ES cells. Furthermore, we show that CatD processing in hippocampi from PScdko mice and littermate controls are identical. As the dissociation of CatD from mannose 6-phosphate receptors in lysosomes requires acidic pH and CatD processing is mediated by proteases with acidic pH optima, we would infer that the absence of PSEN has no significant influence on lysosomal pH. Fourth, we demonstrate that N-linked glycosylation of V0a1 in MEFs or ES cells that lack PSEN is indistinguishable from corresponding WT cells. We confirmed this result by showing that the maturation of V0a1 in the hippocampi of PScdko mice is identical to that in their littermate controls. Hence, these results are entirely consistent with our observation that lysosomal pH and function is unimpaired in the absence of PSEN. Fifth, we failed to observe any differences in N-linked glycosylation of V0a1 and CatD processing in N2a cells or in brains of mice expressing WT or FAD-linked PS1 variants, findings that fail to support the studies reported by Lee et al. (2010). Sixth, we examined the association of PS1 holoprotein with V0a1 and/or STT3B. In this regard, we document that PS1 holoprotein is essentially undetectable at steady-state in both cells and brain; we estimate that the endoproteolytic derivatives of PS1 accumulate to ~0.0025% of total brain protein (Thinakaran et al., 1996). Hence, we chose to use PEN-2 knockdown cell line (Prokop et al., 2004), which has an elevated steady-state level of PS1 holoprotein, or stable N2a cells that overexpress WT human PS1. In neither of these cell lines did we find an association of PS1 holoprotein with either V0a1 or STT3B. Finally, we asked whether V0a1 is a substrate for N-linked glycosylation by STT3B. Indeed, we were intrigued by the model proposed by Lee et al. (2010) because the 834–856 amino acid mouse V0a1 isoforms harbor a single lumenally exposed potential N-linked glycosylation site (asparagine 489) (Nishi and Forgac, 2000;Leng et al., 1999). However, we have shown that N-linked sites that serve as STT3B substrates are generally found at either amino- or carboxyl-terminal regions of the polypeptide backbone (Ruiz-Canada et al., 2009). When tested in cells wherein STT3A or STT3B was depleted, we failed to confirm that V0a1 is uniquely glycosylated by STT3B.
In summary, we now report that cells lacking expression of PS1 either singly, or in combination with PS2 exhibit robust autophagy, and that the loss of PS function neither alters intracellular pH, N-linked glycosylation of V0a1, or processing of CatD. Finally, we fail to confirm the association of PS1 holoprotein with either V0a1 or STT3B. How can we reconcile these findings with those reported by Lee et al. (2010)? We speculate that the PS1ko blastocysts, from which the bulk of the data were obtained by Lee and colleagues that revealed deficits in autophagy, elevated lysosomal pH, inefficient glycosylation of V0a1 and CatD processing, are, in some manner, compromised, perhaps as a consequence of issues related to clonal drift and/or compensation. In this regard, we found it curious that a series of “rescue” experiments intended to demonstrate that human PS1 can reverse the reported phenotypes in PS1ko blastocysts were not conducted in the PS1ko blastocysts, but inexplicably, were performed in PSdko blastocysts. Lee et al. (2010) reported that human PS1 fully “rescues” the deficits in protein degradation, vesicle acidification, macroautophagy responses, V0a1 maturation and CatD processing that existed in PS1ko cells. What was conspicuously absent in this dataset was information pertaining to the aforementioned biological outcomes in naïve PSdko cells, particularly in view of the findings reported herein that failed to reveal a deficit in any of these processes in these cells.
To these latter insights, we assessed the expression of TFEB and genes involved in autophagy, and now report no differences in TFEB mRNA between cultured PSdko-ES and WT-ES cells. Moreover, transcriptome analysis of cultured PS1ko-ES and PSdko-ES cells failed to reveal any significant alteration in the expression of autophagy-specific genes. However, ATG9B expression was elevated in both the cultured PS1ko-ES and PSdko-ES cells, but its is presently unclear as the function of this phagophore/pre-autophagosomal structure (PAS) (Suzuki et al., 2001;Yen and Klionsky, 2007)-associated polytopic membrane protein has not been established. Similarly, transcriptome analysis of PScdko mice failed to uncover any alterations in the expression of TFEB or genes involved in autophagy. Meanwhile, we show a significant elevation in “CLEAR” network genes in the frontal cortices (FC) and hippocampi (HC) of 6 month old PScdko mice compared to littermate controls. In contrast, we observed a small, but significant decrease in the populational expression of “CLEAR” network genes in cultured PS1ko-ES and PSdko-ES cells compared to WT-ES cells. Moreover, with the exception of elevated CTSS transcript levels, the “CLEAR” gene expression changes between PSko-ES cells and PScdko brains do not overlap. While these latter results would seem conflicting, we would argue that the observed differences represent cell type-specific mechanisms that lead to elevated lysosomal function. ES cells are clonal, proliferative, pluripotent stem cells and the response of these cells to PSEN-deficiency maybe significantly different than that observed in the brains of PScdko mice in which PSEN1 is selectively ablated in postmitotic, excitatory neurons. In this regard, while we observe an elevation in steady-state levels of CatD in extracts from PS1ko and PSdko ES cells, this is not due to an increase in CatD mRNA (Fig. 3A, 3B). In contrast, both CatD mRNA and protein are elevated in brains of PScdko mice (Mirnics et al., 2008; Fig. 3C and Table 3). It is instructive that the basal, steady-state levels of the autophagy substrate, LC3-II, are lower in PS1ko and PSdko ES cells compared to WT ES cells, a finding which would suggest that lysosomal hydrolase activity is elevated, leading to enhanced turnover of this substrate in the absence of PS. Indeed, in PS1ko and PSdko cells treated with bafilomycin, the levels of LC3-II are markedly elevated, and autophagic flux is strongly enhanced. Hence, it is conceivable that in PS1ko and PSdko cells, the steady-state levels or catalytic activity of other lysosomal proteases are also elevated without corresponding increases in the mRNAs encoding these polypeptides. This hypothesis remains to be tested.
The mechanism(s) by which loss of PSEN expression elicits an increase in the expression of CLEAR genes in PScdko brains is not fully understood. However, we would offer the tentative proposal that in the absence of PS/γ-secretase activity, the membrane-tethered stubs derived from type 1 membrane proteins that would normally be processed by γ-secretase in endosomal, late Golgi compartments or the plasma membrane would now accumulate in lysosomal compartments wherein the proteases critical for lysosomal function would be “saturated”. As a result, excitatory neurons lacking PSEN expression would respond by inducing CLEAR genes as a compensatory mechanism. We should note that in addition to scoring expression in excitatory neurons, CLEAR gene expression in the PScdko brains is the summation of expression patterns in inhibitory interneurons, microglia, astrocytes, pericytes and endothelial cells. Moreover, and in view of the fact that astro-glial activation is associated with neurodegeneration in 6 month old PScdko brain, other gene expression changes will certainly contribute to the transcriptome.
In any event, the fact that all the genes in the CLEAR network contain TFEB binding site(s), and that TFEB mRNA levels are not impacted by the expression of PS, we can only conclude that in mouse brain, PS regulates pathway(s) critical for lysosomal biogenesis, but in a TFEB-independent manner. The mechanism(s) that underlies this unanticipated, but novel aspect of PS biology remains to be investigated.
Acknowledgements
These studies were supported by NIH R01-AG021494 (SSS and KM), the Adler Foundation (KV), Edward H. Levi Fund (KV and XZ) and Cure Alzheimer’s Fund (SSS). SSS discloses that he is a paid consultant of Nociris Inc. and Eisai Research Labs Inc., but does not hold shares in any company that is a maker or owner of a FDA-regulated drug or device.
  • Ahn K, Shelton CC, Tian Y, Zhang X, Gilchrist ML, Sisodia SS, Li YM. Activation and intrinsic gamma-secretase activity of presenilin 1. Proc Natl Acad Sci U S A. 2010;107:21435–21440. [PubMed]
  • Borchelt DR, Thinakaran G, Eckman CB, Lee MK, Davenport F, Ratovitsky T, Prada CM, Kim G, Seekins S, Yager D, Slunt HH, Wang R, Seeger M, Levey AI, Gandy SE, Copeland NG, Jenkins NA, Price DL, Younkin SG, Sisodia SS. Familial Alzheimer's disease-linked presenilin 1 variants elevate Abeta1–42/1–40 ratio in vitro and in vivo. Neuron. 1996;17:1005–1013. [PubMed]
  • Chen Q, Nakajima A, Choi SH, Xiong X, Sisodia SS, Tang YP. Adult neurogenesis is functionally associated with AD-like neurodegeneration. Neurobiol Dis. 2008;29:316–326. [PMC free article] [PubMed]
  • Choi SH, Veeraraghavalu K, Lazarov O, Marler S, Ransohoff RM, Ramirez JM, Sisodia SS. Non-cell-autonomous effects of presenilin 1 variants on enrichment-mediated hippocampal progenitor cell proliferation and differentiation. Neuron. 2008;59:568–580. [PMC free article] [PubMed]
  • De Strooper Aph-1, Pen-2, and Nicastrin with Presenilin generate an active gamma-Secretase complex. Neuron. 2003;38:9–12. [PubMed]
  • De Strooper B, Annaert W. Novel research horizons for presenilins and gamma-secretases in cell biology and disease. Annu Rev Cell Dev Biol. 2010;26:235–260. [PubMed]
  • Feng R, Rampon C, Tang YP, Shrom D, Jin J, Kyin M, Sopher B, Miller MW, Ware CB, Martin GM, Kim SH, Langdon RB, Sisodia SS, Tsien JZ. Deficient neurogenesis in forebrain-specific presenilin-1 knockout mice is associated with reduced clearance of hippocampal memory traces. Neuron. 2001;32:911–926. [PubMed]
  • Forni PE, Scuoppo C, Imayoshi I, Taulli R, Dastru W, Sala V, Betz UA, Muzzi P, Martinuzzi D, Vercelli AE, Kageyama R, Ponzetto C. High levels of Cre expression in neuronal progenitors cause defects in brain development leading to microencephaly and hydrocephaly. J Neurosci. 2006;26:9593–9602. [PubMed]
  • Hu S, Delorme N, Liu Z, Liu T, Velasco-Gonzalez C, Garai J, Pullikuth A, Koochekpour S. Prosaposin down-modulation decreases metastatic prostate cancer cell adhesion, migration, and invasion. Mol Cancer. 2010;9:30. [PMC free article] [PubMed]
  • Ichimura Y, Kominami E, Tanaka K, Komatsu M. Selective turnover of p62/A170/SQSTM1 by autophagy. Autophagy. 2008;4:1063–1066. [PubMed]
  • Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, Kominami E, Ohsumi Y, Yoshimori T. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 2000;19:5720–5728. [PubMed]
  • Kim SH, Sisodia SS. Evidence that the "NF" motif in transmembrane domain 4 of presenilin 1 is critical for binding with PEN-2. J Biol Chem. 2005;280:41953–41966. [PubMed]
  • Klionsky DJ, Elazar Z, Seglen PO, Rubinsztein DC. Does bafilomycin A1 block the fusion of autophagosomes with lysosomes? Autophagy. 2008;4:849–950. [PubMed]
  • Lah JJ, Heilman CJ, Nash NR, Rees HD, Yi H, Counts SE, Levey AI. Light and electron microscopic localization of presenilin-1 in primate brain. J Neurosci. 1997;17:1971–1980. [PubMed]
  • Lee JH, Yu WH, Kumar A, Lee S, Mohan PS, Peterhoff CM, Wolfe DM, Martinez-Vicente M, Massey AC, Sovak G, Uchiyama Y, Westaway D, Cuervo AM, Nixon RA. Lysosomal proteolysis and autophagy require presenilin 1 and are disrupted by Alzheimer-related PS1 mutations. Cell. 2010;141:1146–1158. [PMC free article] [PubMed]
  • Lee MK, Borchelt DR, Kim G, Thinakaran G, Slunt HH, Ratovitski T, Martin LJ, Kittur A, Gandy S, Levey AI, Jenkins N, Copeland N, Price DL, Sisodia SS. Hyperaccumulation of FAD-linked presenilin 1 variants in vivo. Nat Med. 1997;3:756–760. [PubMed]
  • Lee MK, Slunt HH, Martin LJ, Thinakaran G, Kim G, Gandy SE, Seeger M, Koo E, Price DL, Sisodia SS. Expression of presenilin 1 and 2 (PS1 and PS2) in human and murine tissues. J Neurosci. 1996;16:7513–7525. [PubMed]
  • Leng XH, Nishi T, Forgac M. Transmembrane topography of the 100-kDa a subunit (Vph1p) of the yeast vacuolar proton-translocating ATPase. J Biol Chem. 1999;274:14655–14661. [PubMed]
  • Mirnics K, Norstrom EM, Garbett K, Choi SH, Zhang X, Ebert P, Sisodia SS. Molecular signatures of neurodegeneration in the cortex of PS1/PS2 double knockout mice. Mol Neurodegener. 2008;3:14. [PMC free article] [PubMed]
  • Mizushima N, Yoshimori T. How to interpret LC3 immunoblotting. Autophagy. 2007;3:542–545. [PubMed]
  • Mizushima N, Yoshimori T, Levine B. Methods in mammalian autophagy research. Cell. 2010;140:313–326. [PMC free article] [PubMed]
  • Muzumdar MD, Tasic B, Miyamichi K, Li L, Luo L. A global double-fluorescent Cre reporter mouse. Genesis. 2007;45:593–605. [PubMed]
  • Neely KM, Green KN, LaFerla FM. Presenilin is necessary for efficient proteolysis through the autophagy-lysosome system in a gamma-secretase-independent manner. J Neurosci. 2011;31:2781–2791. [PMC free article] [PubMed]
  • Nishi T, Forgac M. Molecular cloning and expression of three isoforms of the 100-kDa a subunit of the mouse vacuolar proton-translocating ATPase. J Biol Chem. 2000;275:6824–6830. [PubMed]
  • Podlisny MB, Citron M, Amarante P, Sherrington R, Xia W, Zhang J, Diehl T, Levesque G, Fraser P, Haass C, Koo EH, Seubert P, St George-Hyslop P, Teplow DB, Selkoe DJ. Presenilin proteins undergo heterogeneous endoproteolysis between Thr291 and Ala299 and occur as stable N- and C-terminal fragments in normal and Alzheimer brain tissue. Neurobiol Dis. 1997;3:325–337. [PubMed]
  • Prokop S, Shirotani K, Edbauer D, Haass C, Steiner H. Requirement of PEN-2 for stabilization of the presenilin N-/C-terminal fragment heterodimer within the gamma-secretase complex. J Biol Chem. 2004;279:23255–23261. [PubMed]
  • Ratovitski T, Slunt HH, Thinakaran G, Price DL, Sisodia SS, Borchelt DR. Endoproteolytic processing and stabilization of wild-type and mutant presenilin. J Biol Chem. 1997;272:24536–24541. [PubMed]
  • Rubinsztein DC, Cuervo AM, Ravikumar B, Sarkar S, Korolchuk V, Kaushik S, Klionsky DJ. In search of an "autophagomometer". Autophagy. 2009;5:585–589. [PubMed]
  • Ruiz-Canada C, Kelleher DJ, Gilmore R. Cotranslational and posttranslational N-glycosylation of polypeptides by distinct mammalian OST isoforms. Cell. 2009;136:272–283. [PMC free article] [PubMed]
  • Sardiello M, Palmieri M, di RA, Medina DL, Valenza M, Gennarino VA, Di MC, Donaudy F, Embrione V, Polishchuk RS, Banfi S, Parenti G, Cattaneo E, Ballabio A. A gene network regulating lysosomal biogenesis and function. Science. 2009;325:473–477. [PubMed]
  • Saura CA, Choi SY, Beglopoulos V, Malkani S, Zhang D, Shankaranarayana Rao BS, Chattarji S, Kelleher RJ, III, Kandel ER, Duff K, Kirkwood A, Shen J. Loss of presenilin function causes impairments of memory and synaptic plasticity followed by age-dependent neurodegeneration. Neuron. 2004;42:23–36. [PubMed]
  • Semprini S, Troup TJ, Kotelevtseva N, King K, Davis JR, Mullins LJ, Chapman KE, Dunbar DR, Mullins JJ. Cryptic loxP sites in mammalian genomes: genome-wide distribution and relevance for the efficiency of BAC/PAC recombineering techniques. Nucleic Acids Res. 2007;35:1402–1410. [PMC free article] [PubMed]
  • Settembre C, Di MC, Polito VA, Garcia AM, Vetrini F, Erdin S, Erdin SU, Huynh T, Medina D, Colella P, Sardiello M, Rubinsztein DC, Ballabio A. TFEB links autophagy to lysosomal biogenesis. Science. 2011;332:1429–1433. [PMC free article] [PubMed]
  • Sherrington R, et al. Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease. Nature. 1995;375:754–760. [PubMed]
  • Shintani T, Klionsky DJ. Autophagy in health and disease: a double-edged sword. Science. 2004;306:990–995. [PMC free article] [PubMed]
  • Suzuki K, Kirisako T, Kamada Y, Mizushima N, Noda T, Ohsumi Y. The preautophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation. EMBO J. 2001;20:5971–5981. [PubMed]
  • Takasugi N, Tomita T, Hayashi I, Tsuruoka M, Niimura M, Takahashi Y, Thinakaran G, Iwatsubo T. The role of presenilin cofactors in the gamma-secretase complex. Nature. 2003;422:438–441. [PubMed]
  • Tanida I, Minematsu-Ikeguchi N, Ueno T, Kominami E. Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy. 2005;1:84–91. [PubMed]
  • Thinakaran G, Borchelt DR, Lee MK, Slunt HH, Spitzer L, Kim G, Ratovitsky T, Davenport F, Nordstedt C, Seeger M, Hardy J, Levey AI, Gandy SE, Jenkins NA, Copeland NG, Price DL, Sisodia SS. Endoproteolysis of presenilin 1 and accumulation of processed derivatives in vivo. Neuron. 1996;17:181–190. [PubMed]
  • Thinakaran G, Harris CL, Ratovitski T, Davenport F, Slunt HH, Price DL, Borchelt DR, Sisodia SS. Evidence that levels of presenilins (PS1 and PS2) are coordinately regulated by competition for limiting cellular factors. J Biol Chem. 1997;272:28415–28422. [PubMed]
  • Thyagarajan B, Guimaraes MJ, Groth AC, Calos MP. Mammalian genomes contain active recombinase recognition sites. Gene. 2000;244:47–54. [PubMed]
  • Yen WL, Klionsky DJ. Atg27 is a second transmembrane cycling protein. Autophagy. 2007;3:254–256. [PubMed]
  • Zaidi N, Maurer A, Nieke S, Kalbacher H. Cathepsin D: a cellular roadmap. Biochem Biophys Res Commun. 2008;376:5–9. [PubMed]
  • Zhang Z, Nadeau P, Song W, Donoviel D, Yuan M, Bernstein A, Yankner BA. Presenilins are required for gamma-secretase cleavage of beta-APP and transmembrane cleavage of Notch-1. Nat Cell Biol. 2000;2:463–465. [PubMed]