Attenuation of β-Catenin/TCF Target Gene Activation in DKO mESCs Does Not Restore Their Ability to Give Rise to Neurectoderm in a Defined Neural Differentiation Assay
(A) Morphology of DKO, WT, DKO-GSK3β, and DKO-TCF4DN mESCs 9 days after the initiation of neural differentiation in N2B27 medium.
(B and C) The indicated mESC lines were subjected to western blot (B) and qRT-PCR (C) analyses at 9 and 14 days after being seeded in N2B27 medium. WT and DKO-GSK3β mESCs exhibited robustly increased expression of the neuronal markers β-III-tubulin, Map2, and Pax6 and reduced expression of Oct-4, Nanog, and Sox2, whereas DKO and DKO-TCF4DN mESCs retained Oct-4, Nanog, and Sox2 and failed to express β-III-tubulin, Map2, and Pax6. Bars represent n = 3 ± SEM.