Search tips
Search criteria 


Logo of jbcThe Journal of Biological Chemistry
J Biol Chem. 2012 October 5; 287(41): 34499.
PMCID: PMC3464557

Domains II and III of Bacillus thuringiensis Cry1Ab toxin remain exposed to the solvent after insertion of part of domain I into the membrane.

VOLUME 286 (2011) PAGES 19109–19117

PAGE 19111:

An error has been detected in the methodology description of “Insertion of Cry1Ab Mutant Toxin into the BBMV.” The samples used in Western blot assays were not boiled. Instead, they were incubated for 5 min at 37 °C before loading into 10% SDS-PAGE. A new supplemental Fig. S1 is presented.

Western blot analysis of membrane associated proteins. Mutant proteins were incubated with BBMV at pH 9.0. Toxin proteins inserted into the BBMV were recovered by centrifugation. Pellets were suspended in Laemmli sample buffer, incubated 5 min at 37 °C, ...

Articles from The Journal of Biological Chemistry are provided here courtesy of American Society for Biochemistry and Molecular Biology