PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Adv Exp Med Biol. Author manuscript; available in PMC Oct 5, 2012.
Published in final edited form as:
PMCID: PMC3464500
NIHMSID: NIHMS407293
Multiprotein Complexes of Retinitis Pigmentosa GTPase Regulator (RPGR), a Ciliary Protein Mutated in X-Linked Retinitis Pigmentosa (XLRP)
Carlos Murga-Zamalloa, Anand Swaroop, and Hemant Khannacorresponding author
Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, Ann Arbor, MI 48105, USA
corresponding authorCorresponding author.
Hemant Khanna: hkhanna/at/med.umich.edu
Mutations in Retinitis Pigmentosa GTPase Regulator (RPGR) are a frequent cause of X-linked Retinitis Pigmentosa (XLRP). The RPGR gene undergoes extensive alternative splicing and encodes for distinct protein isoforms in the retina. Extensive studies using isoform-specific antibodies and mouse mutants have revealed that RPGR predominantly localizes to the transition zone to primary cilia and associates with selected ciliary and microtubule-associated assemblies in photoreceptors. In this chapter, we have summarized recent advances on understanding the role of RPGR in photoreceptor protein trafficking. We also provide new evidence that suggests the existence of discrete RPGR multiprotein complexes in photoreceptors. Piecing together the RPGR-interactome in different subcellular compartments should provide critical insights into the role of alternative RPGR isoforms in associated orphan and syndromic retinal degenerative diseases.
XLRP is a relatively severe form of retinal degeneration, accounting for 10–20% of all RP (Bird 1975; Fishman 1988). Most affected males exhibit early-onset visual symptoms with night-blindness in the first decade and rapid progression towards blindness by age 40 (Bird 1975; Fishman et al. 1978). Heterozygous carrier females can show electroretinographic (ERG) abnormalities and tapetal reflex (Fishman et al. 1986). Some XLRP patients have abnormal sperm phenotype (Hunter et al. 1988) or hearing defects (Iannaccone et al. 2004; Zito et al. 2003). To date, six genetic loci have been mapped: RP2, RP3, RP6, RP23, RP24 and RP34 (Fujita et al. 1996; Gieser et al. 1998; Hardcastle et al. 2000; McGuire et al. 1995; Melamud et al. 2006; Wright et al. 1991). The genes for two major forms of XLRP, RP2 [Schwahn et al. 1998] and RP3 [RPGR (Meindl et al. 1996; Roepman et al. 1996)], have been cloned.
Mutations in RP2 account for approximately 10% of XLRP (Breuer et al. 2002; Hardcastle et al. 1999; Mears et al. 1999; Sharon et al. 2003). The RP2 gene encodes a putative protein of 350 amino acids (Chapple et al. 2000; Schwahn et al. 1998). The crystal structure of the RP2 protein reveals an amino-terminal β-helix that is structurally and functionally homologous to the tubulin-specific chaperone, cofactor C (TBCC); most disease-causing missense mutations are present in this domain (Bartolini et al. 2002; Grayson et al. 2002; Kuhnel et al. 2006). RP2 interacts with ADP-ribosylation factor-like 3 (ARL3) (Kuhnel et al. 2006), a microtubule-associated small GTP-binding protein (Kahn et al. 2005) that localizes to the sensory cilium of photoreceptors (Grayson et al. 2002). However, the precise role of RP2 in photoreceptors has not been delineated.
Mutations in the RPGR gene account for over 70% of XLRP and as much as 25% of simplex RP males (Breuer et al. 2002; Shu et al. 2007). Initial analysis of a ubiquitously-expressed RPGREx1–19 transcript (derived from exons 1–19; 815 amino acids) identified mutations in only 10–20% of XLRP patients and families (Buraczynska et al. 1997; Fujita et al. 1997; Meindl et al. 1996; Roepman et al. 1996; Sharon et al. 2000). The discovery of an alternative transcript with a purine-rich terminal exon ORF15, which included a part of the original intron 15 (called RPGRORF15) revealed additional mutations in almost 50% of individuals with XLRP (Breuer et al. 2002; Sharon et al. 2003; Vervoort et al. 2000). Mutations in RPGRORF15 have also been identified in patients with cone-rod dystrophy, atrophic macular degeneration, and Coat’s-like exudative vasculopathy (Ayyagari et al. 2002; Demirci et al. 2006; Demirci et al. 2002; Sharon et al. 2003; Yang et al. 2002). Some individuals with RPGR mutations are reported to show a syndromic phenotype that may include respiratory tract infections, hearing loss, and primary cilia dyskinesia (Iannaccone et al. 2004; Koenekoop et al. 2003; Moore et al. 2006; van Dorp et al. 1992; Zito et al. 2003). In addition, patients with mutations in RPGR exons 2–14 appear to display a more severe clinical phenotype than those with exon ORF15 mutations (Sharon et al. 2003). However, further genotype-phenotype studies are needed to elucidate the clinical heterogeneity associated with RPGR mutations.
The N-terminal region of RPGR contains tandem repeats (termed RCC1-like domain; RLD) homologous to RCC1, which is a guanine nucleotide exchange factor (GEF) for Ran-GTPase that is involved in nucleo-cytoplasmic transport (Meindl et a1. 1996; Renault et al. 2001). Complex splicing patterns are reported for RPGR though the physiological relevance of these transcripts is unclear (Hong and Li 2002; Kirschner et al. 1999; Vervoort et al. 2000; Yan et al. 1998). Multiple immunoreactive bands are observed using isoform-specific RPGR antibodies (Chang et al. 2006; He et al. 2008; Hong and Li 2002; Khanna et al. 2005; Mavlyutov et al. 2002; Otto et al. 2005; Shu et al. 2005; Yan et al. 1998).
Several different groups have reported the localization of RPGR in the retina. Initially RPGR was shown to localize to the photoreceptor cilium independent of the species tested (Hong et al. 2003); however, another study demonstrated species-specific differences in RPGR localization (Mavlyutov et al. 2002). By immunogold labeling, we demonstrated the RPGRORF15 protein in the transition zone and basal bodies of both mouse and human photoreceptor cilia though some additional labeling was detected in the inner and outer segments (Khanna et al. 2005; Shu et al. 2005). In proliferating cells, centrioles were labeled with anti-RPGR antibodies (He et al. 2008; Shu et al. 2005). It should be noted that primary cilia arise from mother centrioles in post-mitotic cells (Pedersen et al. 2008). Distinct localization of RPGRORF15 isoforms may reflect their relative abundance in distinct subcellular compartments of photoreceptors.
A knockout (ko) mouse with deletion of exons 4–6 of Rpgr was reported to show late-onset cone-rod degeneration (Hong et al. 2000); however, this Rpgr-ko mouse is not a complete null and expresses some specific RPGRORF15 isoforms that localize to the transition zone of photoreceptor cilia (Khanna et al. 2005). Interestingly, ectopic expression of an ORF15 variant could partially rescue the Rpgr-ko phenotype (Hong et al. 2005), or behave as a dominant gain-of-function variant resulting in rapid disease progression (Hong et al. 2004). Attempts to generate a complete Rpgr null mutation in mouse have not been successful. Notably, frameshift mutations in RPGRORF15 have been identified in two naturally-occurring canine mutants; the XLPRA2 dog exhibits relatively rapid photoreceptor degeneration and severe ERG abnormalities, whereas the XLPRA1 mutant shows a milder phenotype (Beltran et al. 2006; Zhang et al. 2002). Aberrant behavior of the two mutant ORF15 proteins in cultured cells may reflect the phenotypic differences in the two canine models (Zhang et al. 2002).
Photoreceptor outer segment (OS) membrane discs and inner segment (IS) are linked by a sensory cilium (CC), which is a modified primary cilium (Young 1968). Ciliogenesis involves an evolutionarily conserved process, called Intraflagellar Transport (IFT) (Rosenbaum et al. 1999). In photoreceptors, components of the IFT complex and cargo proteins are synthesized in the IS, docked at the basal body, and transported distally by the anterograde heterotrimeric motor, Kinesin-II (Besharse et al. 2003). The IFT components are believed to be replenished by their transport back to the basal body by a presumptive retrograde motor cytoplasmic dynein 1b/2 (Besharse et al. 2003).
Vertebrate photoreceptors are highly metabolically active; approximately 10% of OS disks are turned over each day (Bok and Young 1972; Young 1968). It is estimated that ~2000 opsin molecules are transported to the OS per minute in an adult human retina (Besharse 1986; Williams 2002). The opsin molecules are synthesized in the IS and are targeted to the basal body, where they are fused with the ciliary membrane and trafficked distally to the OS (Deretic et al. 2005). Hence, it is not surprising that perturbations in ciliary transport of opsins or OS biogenesis are associated with severe retinal degeneration and blindness (Insinna and Besharse 2008).
IFT-mediated transport of rhodopsin and other signaling proteins is critical for photoreceptor survival and function. Conditional Kif3a−/− mice and Tg737orpk, a hypomorphic allele of IFT88, result in opsin accumulation in the IS (Marszalek et al. 2000; Pazour et al. 2002). A number of retinal disease proteins CEP290/NPHP6, RPGRIP1 and RP1 are required for cilia-dependent OS transport, generation or maintenance (Chang et al. 2006; Liu et al. 2004; Zhao et al. 2003). Several pleiotropic disorders, such as Senior-Loken Syndrome, Joubert Syndrome, and Bardet-Biedl Syndrome, are also caused by mutations in ciliary proteins and share retinopathy as a common phenotype (Badano et al. 2006).
RPGR exists in macromolecular complexes with other proteins in photoreceptors. Two proteins were initially identified by yeast two-hybrid analysis using the RPGR-RLD as bait: RPGRIP1, which is localized to the sensory cilium and mutated in retinopathy patients (Boylan and Wright 2000; Dryja et al. 2001; Hong et al. 2001); and delta subunit of rod cyclic guanosine monophosphate phosphodiesterase (PDE6D), a prenyl-binding protein involved in the retrieval of PDE from rod outer segment membranes by interacting with Rab13 (Linari et al. 1999; Zhang et al. 2004). Two chromosome-associated proteins SMC1 and SMC3 (Hirano 2006; Khanna et al. 2005; Liu et al. 2007) and two ciliary disease-associated proteins, NPHP5 (Otto et al. 2005) and CEP290/NPHP6 (Chang et al. 2006; Sayer et al. 2006) are also reported to be a part of the RPGRORF15 macromolecular complexes. In addition, RPGR can associate with Tg737/Polaris/IFT88 (Davenport and Yoder 2005; Pazour et al. 2002) and several microtubule transport proteins (Khanna et al. 2005).
We hypothesize that RPGR isoforms are partitioned in distinct multiprotein complexes in photoreceptors. To evaluate this hypothesis, we have carried out sequential immunodepletion experiments. We initially used antibodies against two of the RPGR partners, CEP290/NPHP6 and SMC1, in order to immunodeplete RPGR that is part of these complexes from the retinal ciliary extract preparation. The remaining supernatant was subjected to immunoprecipitation (IP) with the anti-RPGRORF15 antibody, followed by immunoblotting to test for the presence or absence of remaining RPGRORF15-interacting proteins (Fig. 13.1a). Even after immunodepletion of CEP290 from the retinal ciliary fraction (Fig. 13.1b), RPGR was still associated with IFT88, KIF3A, and γ-tubulin, but not with SMC1 and SMC3 (Fig. 13.1c). This data suggests that RPGR’s complex with CEP290, SMC1, and SMC3 is distinct from that with IFT88, KIF3A, and γ-tubulin. On the other hand, after SMC1 immunodepletion, RPGR antibody could immunoprecipitate only a fraction of CEP290 from retinal ciliary extract. Similar results were obtained with SMC3 (data not shown).
Fig. 13.1
Fig. 13.1
(a) Schematic representation of the strategy to dissect distinct RPGR-containing multiprotein complexes in photoreceptor cilia. IP: Immunoprecipitation; Ab: antibody, (b) Protein extract (~150 µg) was subjected to immunoprecipitation using indicated (more ...)
These observations indicate that RPGR exists in at least three distinct complexes: first with IFT88, KIF3A, and γ-tubulin; second with CEP290, SMC1, and SMC3 and; third with CEP290 and probably other ciliary proteins (Fig. 13.2). Future detailed analysis of these and additional complexes should assist in dissecting the RPGR function in photoreceptors.
Fig. 13.2
Fig. 13.2
Schematic representation of the putative distinct RPGR complexes that can exist in photoreceptors. Proteins A and B represent as yet unidentified molecular partners that can be part of such complexes
Despite extensive investigations, the underlying mechanism of ciliary transport-associated photoreceptor dysfunction is poorly understood at this stage. We suggest that RPGR-defect could occur at multiple stages: (a) cargo loading onto the vesicles; (b) vesicular trafficking towards the basal body, (c) docking of the cargo-laden vesicles at the basal body, (d) selection of cargo and transfer to the IFT complex, or (e) anterograde transport towards the distal OS. We reckon that the different RPGR complexes may participate in some or all of these transport processes. Given the importance of these pathways in photoreceptor development and survival, mutations in RPGR may disrupt its interactome thereby leading to retinal degeneration.
Acknowledgments
This work is supported by the grants from the National Eye Institute (RO1-EY007961). Midwest Eye Banks and Transplantation Center, and by NEI/NIH intramural program.
Footnotes
Retinitis Pigmentosa (RP: MIM 268000) is a leading cause of inherited blindness in developed countries. RP refers to a group of debilitating neurodegenerative diseases with clinically heterogeneous findings, which include bone spicule like pigmentary deposits in the retina, progressive loss of peripheral vision and eventually deterioration of central vision due to cone loss (Bird 1987; Fishman et al. 1988; Heckenlively et al. 1988; Sullivan and Daiger 1996). Over 30 RP genes have been identified so far (http://www.sph.uth.tmc.edu/Retnet) (Hartong et al. 2006). No effective approach exists for the management or treatment of RP.
  • Ayyagari R, Demirci FY, Liu J, et al. X-linked recessive atrophic macular degeneration from RPGR mutation. Genomics. 2002;80(2):166–171. [PubMed]
  • Badano JL, Mitsuma N, Beales PL, et al. The ciliopathies: an emerging class of human genetic disorders. Annu Rev Genomics Hum Genet. 2006;7:125–148. [PubMed]
  • Bartolini F, Bhamidipati A, Thomas S, et al. Functional overlap between retinitis pigmentosa 2 protein and the tubulin-specific chaperone cofactor C. J Biol Chem. 2002;277(17):14629–14634. [PubMed]
  • Beltran WA, Hammond P, Acland GM, et al. A frameshift mutation in RPGR exon ORF15 causes photoreceptor degeneration and inner retina remodeling in a model of X-linked retinitis pigmentosa. Invest Ophthalmol Vis Sci. 2006;47(4):1669–1681. [PubMed]
  • Besharse JC. The Retina: a model for cell biological studies Part I. New York: Academic; 1986. pp. 297–352.
  • Besharse JC, Baker SA, Luby-Phelps K, et al. Photoreceptor intersegmental transport and retinal degeneration: a conserved pathway common to motile and sensory cilia. Adv Exp Med Biol. 2003;553:157–164. [PubMed]
  • Bird AC. X-linked retinitis pigmentosa. Br J Ophthalmol. 1975;59(4):177–199. [PMC free article] [PubMed]
  • Bird AC. Clinical investigation of retinitis pigmentosa. Prog Gin Biol Res. 1987;247:3–20. [PubMed]
  • Bok D, Young RW. The renewal of diffusely distributed protein in the outer segments of rods and cones. Vision Res. 1972;12(2):161–168. [PubMed]
  • Boylan JP, Wright AF. Identification of a novel protein interacting with RPGR. Hum Mol Genet. 2000;9(14):2085–2093. [PubMed]
  • Breuer DK, Yashar BM, Filippova E, et al. A comprehensive mutation analysis of RP2 and RPGR in a North American cohort of families with X-linked retinitis pigmentosa. Ann J Hum Genet. 2002;70(6):1545–1554. [PubMed]
  • Buraczynska M, Wu W, Fujita R, et al. Spectrum of mutations in the RPGR gene that are identified in 20% of families with X-linked retinitis pigmentosa. Am J Hum Genet. 1997;61(6):1287–1292. [PubMed]
  • Chang B, Khanna H, Hawes N, et al. In-frame deletion in a novel centrosomal/ciliary protein CEP290/NPHP6 perturbs its interaction with RPGR and results in early-onset retinal degeneration in the rd16 mouse. Hum Mol Genet. 2006;15(11):1847–1857. [PMC free article] [PubMed]
  • Chapple JP, Hardcastle AJ, Grayson C, et al. Mutations in the N-terminus of the X-linked retinitis pigmentosa protein RP2 interfere with the normal targeting of the protein to the plasma membrane. Hum Mol Genet. 2000;9(13):1919–1926. [PubMed]
  • Davenport JR, Yoder BK. An incredible decade for the primary cilium; a look at a once-forgotten organelle. Am J Physiol Renal Physiol. 2005;289(6):F1159–F1169. [PubMed]
  • Demirci FY, Rigatti BW, Mah TS, et al. A novel RPGR exon ORF15 mutation in a family with X-linked relinitis pigmentosa and Coats′-like exudative vasculopathy. Am J Ophthalmol. 2006;141(1):208–210. [PubMed]
  • Demirci FY, Rigatti BW, Wen G, et al. X-linked cone-rod dystrophy (locus COD1): identification of mutations in RPGR exon ORF15. Am J Hum Genet. 2002;70(4):1049–1053. [PubMed]
  • Deretic D, Williams AH, Ransom N, et al. Rhodopsin C terminus, the site of mutations causing retinal disease, regulates trafficking by binding to ADP-ribosylation factor 4 (ARF4) Proc Natl Acad Sci USA. 2005;102(9):3301–3306. [PubMed]
  • Dryja TP, Adams SM, Grimsby JL, et al. Null RPGRIP1 alleles in patients with Leber congenital amaurosis. Am J Hum Genet. 2001;68(5):1295–1298. [PubMed]
  • Fishman GA. Retinitis pigmentosa. Genetic percentages. Arch Ophthalmol. 1978;96(5):822–826. [PubMed]
  • Fishman GA, Farber MD, Derlacki DJ. X-linked retinitis pigmentosa. Profile of clinical findings. Arch Ophthalmol. 1958;106(3):369–375. [PubMed]
  • Fishman GA, Weinbevg AB, McMahon TT. X-linked recessive retinitis pigmentosa. Clinical characteristics of carriers. Arch Ophthalmol. 1986;104(9):1329–1335. [PubMed]
  • Fujita R, Bingham E, Forsythe P, et al. A recombination outside the BB deletion refines the location of the X linked retinitis pigmentosa locus RP3. Am J Hum Genet. 1996;59(1):152–158. [PubMed]
  • Fujita R, Buraczynska M, Gieser L, et al. Analysis of the RPGR gene in 11 pedigrees with the retinitis pigmentosa type 3 genotype: paucity of mutations in the coding region but splice defects in two families. Am J Hum Genet. 1997;61(3):571–580. [PubMed]
  • Gieser L, Fujita R, Goring HH, et al. A novel locus (RP24) for X-linked retinitis pigmentosa maps to Xq26-27. Am J Hum Genet. 1998;63(5):1439–1447. [PubMed]
  • Grayson C, Bartolini F, Chapple JP. Localization in the human retina of the X-linked retinitis pigmentosa protein RP2, its homologue cofactor C and the RP2 interacting protein Arl3. Hum Mol Genet. 2002;11(24):3065–3074. [PubMed]
  • Hardcastle AJ, Thiselton DL, Van Maldergem L, et al. Mutations in the RP2 gene cause disease in 10% of families with familial X-linked retinitis pigmentosa assessed in this study. Am J Hum Genet. 1999;64(4):1210–1215. [PubMed]
  • Hardcastle AJ, Thiselton DL, Zito I, et al. Evidence for a new locus for X-linked retinitis pigmentosa (RP23) Invest Ophthalmol Vis Sci. 2000;41(8):2080–2086. [PubMed]
  • Hartong DT, Berson EL, Dryja TP. Retinitis pigmentosa. Lancet. 2006;368(9549):1795–1809. [PubMed]
  • He S, Parapuram SK, Hurd TW, et al. Retinitis Pigmentosa GTPase Regulator (RPGR) protein isoforms in mammalian retina: insights into X-linked Retinitis Pigmentosa and associated ciliopathies. Vision Res. 2008;48(3):366–376. [PMC free article] [PubMed]
  • Heckenlively JR, Yoser SL, Friedman LH, et al. Clinical findings and common symptoms in retinitis pigmentosa. Am J Ophthalmol. 1988;105(5):504–511. [PubMed]
  • Hirano T. At the heart of the chromosome: SMC proteins in action. Nat Rev Mol Cell Biol. 2006;7(5):311–322. [PubMed]
  • Hong DH, Li T. Complex expression pattern of RPGR reveals a role for purine-rich exonic splicing enhancers. Invest Ophthalmol Vis Sci. 2002;43(11):3373–3382. [PubMed]
  • Hong DH, Pawlyk BS, Adamian M, et al. Dominant, gain-of-function mutant produced by truncation of RPGR. Invest Ophthalmol Vis Sci. 2004;45(1):36–41. [PubMed]
  • Hong DH, Pawlyk BS, Adamian M, et al. A single, abbreviated RPGR-ORF15 variant reconstitutes RPGR function in vivo. Invest Ophthalmol Vis Sci. 2005;46(2):435–441. [PubMed]
  • Hong DH, Pawlyk BS, Shang J. A retinitis pigmentosa GTPase regulator (RPGR)-deficient mouse model for X-linked retinitis pigmentosa (RP3) Proc Natl Acad Sci USA. 2000;97(7):3649–3654. [PubMed]
  • Hong DH, Pawlyk B, Sokolov M, et al. RPGR isoforms in photoreceptor connecting cilia and the transitional zone of motile cilia. Invest Ophthalmol Vis Sci. 2003;44(6):2413–2421. [PubMed]
  • Hong DH, Yue G, Adamian M, et al. Retinitis pigmentosa GTPase regulator (RPGRr)-interacting protein is stably associated with the photoreceptor ciliary axonetne and anchors RPGR to the connecting cilium. J Biol Chem. 2001;276(15):12091–12099. [PubMed]
  • Hunter DG, Fishman GA, Kretzer FL. Abnormal axonemes in X-linked retinitis pigmentosa. Arch Ophthalmol. 1988;106(3):362–368. [PubMed]
  • Iannaccone A, Wang X, Jablonski MM, et al. Increasing evidence for syndromic phenotypes associated with RPGR mutations. Am J Ophthalmol. 2004;137(4):785–786. author reply 786. [PubMed]
  • Insinna C, Besharse JC. Intraflagellar transport and the sensory outer segment of vertebrate photoreceptors. Dev Dyn. 2008;237(8):1982–1992. [PMC free article] [PubMed]
  • Kahn RA, Volpicelli-Daley L, Bowzard B, et al. Arf family GTPases: roles in membrane traffic and microtubule dynamics. Biochem Soc Trans. 2005;33(Pt 6):1269–1272. [PubMed]
  • Khanna H, Hurd TW, Lillo C, et al. RPGR-ORF15, which is mutated in retinitis pigmentosa, associates with SMC1, SMC3, and microtubule transport proteins. J Biol Chem. 2005;280(39):33580–33587. [PMC free article] [PubMed]
  • Kirschner R, Rosenberg T, Schultz-Heienbrok R, et al. RPGR transcription studies in mouse and human tissues reveal a retina-specific isoform that is disrupted in a patient with X-linked retinitis pigmentosa. Hum Mol Genet. 1999;8(8):1571–1578. [PubMed]
  • Koenekoop RK, Loyer M, Hand CK, et al. Novel RPGR mutations with distinct retinitis pigmentosa phenotypes in French-Canadian families. Am J Ophthalmol. 2003;136(4):675–687. [PubMed]
  • Kuhnel K, Veltel S, Schlichting I, et al. Crystal structure of the human retinitis pigmentosa 2 protein and its interaction with Arl3. Structure. 2006;14(2):367–378. [PubMed]
  • Linari M, Ueffing M, Manson F, et al. The retinitis pigmentosa GTPase regulator, RPGR, interacts with the delta subunit of rod cyclic GMP phosphodiesterase. Proc Natl Acad Sci USA. 1999;96(4):1315–1320. [PubMed]
  • Liu Q, Tan G, Levenkova N, et al. The proteome of the mouse photoreceptor sensory cilium complex. Mol Cell Proteomics. 2007;6(8):1299–1317. [PMC free article] [PubMed]
  • Liu Q, Zuo J, Pierce EA. The retinitis pigmentosa 1 protein is a photoreceptor microtubule-associated protein. J Neurosci. 2004;24(29):6427–6436. [PMC free article] [PubMed]
  • Marszalek JR, Liu X, Roberts EA, et al. Genetic evidence for selective transport of opsin and arrestin by kinesin-II in mammalian photoreceptors. Cell. 2000;102(2):175–187. [PubMed]
  • Mavlyutov TA, Zhao H, Ferreira PA. Species-specific subcellular localization of RPGR and RPGRIP isoforms: implications for the phenotypic variability of congenital retinopathies among species. Hum Mol Genet. 2002;11(16):1899–1907. [PubMed]
  • McGuire RE, Sullivan LS, Blanton SH, et al. X-linked dominant cone-rod degeneration: linkage mapping of a new locus for retinitis pigmentosa (RP 15) to Xp22.13–p22.11. Am J Hum Genet. 1995;57(1):87–94. [PubMed]
  • Mears AJ, Gieser L, Yan D, et al. Protein-truncation mutations in the RP2 gene in a North American cohort of families with X-linked retinitis pigmentosa. Am J Hum Genet. 1999;64(3):897–900. [PubMed]
  • Meindl A, Dry K, Herrmann K, et al. A gene (RPGR) wkh homology to the RCC1 guanine nucleotide exchange factor is mutated in X-linked retinitis pigmentosa (RP3) Nat Genet. 1996;35(1):42. [PubMed]
  • Melamud A, Shen GQ, Chung D, et al. Mapping a new genetic locus for X linked retinitis pigmentosa to Xq28. J Med Genet. 2006;43(6):e27. [PMC free article] [PubMed]
  • Moore A, Escudier E, Roger G, et al. RPGR is mutated in patients with a complex X linked phenotype combining primary ciliary dyskinesia and retinitis pigmentosa. J Med Genet. 2006;43(4):326–333. [PMC free article] [PubMed]
  • Otto EA, Loeys B, Khanna H, et al. Nephrocystin-5, a ciliary IQ domain protein, is mutated in Senior-Loken syndrome and interacts with RPGR and calmodulin. Nat Genet. 2005;37(3):282–288. [PubMed]
  • Pazour GJ, Baker SA, Deane JA, et al. The intraflagellar transport protein, IFT88, is essential for vertebrate photoreceptor assembly and maintenance. J Cell Biol. 2002;157(1):103–113. [PMC free article] [PubMed]
  • Pedersen LB, Veland IR, Schroder JM, et al. Assembly of primary cilia. Dev Dyn. 2008;237(8):1993–2006. [PubMed]
  • Renault L, Kuhlmann J, Henkel A, et al. Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1) Cell. 2001;105(2):245–255. [PubMed]
  • Roepman R, van Duijnhoven G, Rosenberg T, et al. Positional cloning of the gene for X-linked retinitis pigmentosa 3: homology with the guanine-nucleotide-exchange factor RCC1. Hum Mol Genet. 1996;5(7):1035–1041. [PubMed]
  • Rosenbaum JL, Cole DG, Diener DR. Intraflagellar transport: the eyes have it. J Cell Biol. 1999;144(3):385–388. [PMC free article] [PubMed]
  • Sayer JA, Otto EA, O’Toole J, et al. The centrosomal protein nephrocystin-6 is mutated in Joubert syndrome and activates transcription factor ATF4. Nat Genet. 2006;38(6):674–681. [PubMed]
  • Schwahn U, Lenzner S, Dong J. Positional cloning of the gene for X-linked retinitis pigmentosa 2. Nat Genet. 1998;19(4):327–332. [PubMed]
  • Sharon D, Bruns GA, McGee TL, et al. X-linked retinitis pigmentosa: mutation spectrum of the RPGR and RP2 genes and correlation with visual function. Invest Ophthalmol Vis Sci. 2000;41(9):2712–2721. [PubMed]
  • Sharon D, Sandberg MA, Rabe VW, et al. RP2 and RPGR mutations and clinical correlations in patients with X-linked retinitis pigmentosa. Am J Hum Genet. 2003;73(5):1131–1146. [PubMed]
  • Shu X, Black GC, Rice JM, et al. RPGR mutation analysis and disease: an update. Hum Mutat. 2007;28(4):322–328. [PubMed]
  • Shu X, Fry AM, Tulloch B, et al. RPGR ORF15 isoform co-localizes with RPGRIP1 at centrioles and basal bodies and interacts with nucleophosmin. Hum Mol Genet. 2005;14(9):1183–1197. [PubMed]
  • Sullivan LS, Daiger SP. Inherited retinal degeneration: exceptional genetic and clinical heterogeneity. Mol Med Today. 1996;2(9):380–386. [PubMed]
  • van Dorp DB, Wright AF, Carothers AD, et al. A family with RP3 type of X-linked retinitis pigmentosa: an association with ciliary abnormalities. Hum Genet. 1992;88(3):331–334. [PubMed]
  • Vervoort R, Lennon A, Bird AC, et al. Mutational hot spot within a new RPGR exon in X-linked retinitis pigmentosa. Nat Genet. 2000;25(4):462–466. [PubMed]
  • Williams DS. Transport to the photoreceptor outer segment by myosin VIIa and kinesin II. Vision Res. 2002;42(4):455–462. [PubMed]
  • Wright AF, Bhattacharya SS, Aldred MA, et al. Genetic localisation of the RP2 type of X linked retinitis pigmentosa in a large kindred. J Med Genet. 1991;28(7):453–457. [PMC free article] [PubMed]
  • Yan D, Swain PK, Breuer D, et al. Biochemical characterization and subcellular localization of the mouse retinitis pigmentosa GTPase regulator (mRpgr) J Biol Chem. 1998;273(31):19656–19663. [PubMed]
  • Yang Z, Peachey NS, Moshfeghi DM, et al. Mutations in the RPGR gene cause X-linked cone dystrophy. Hum Mol Genet. 2002;11(5):605–611. [PubMed]
  • Young RW. Passage of newly formed protein through the connecting cilium of retina rods in the frog. J Ultrastruct Res. 1968;23(5):462–473. [PubMed]
  • Zhang Q, Acland GM, Wu WX, et al. Different RPGR exon ORF15 mutations in Canids provide insights into photoreceptor cell degeneration. Hum Mol Genet. 2002;11(9):993–1003. [PubMed]
  • Zhang H, Liu XH, Zhang K, et al. Photoreceptor cGMP phosphodiesterase delta subunit (PDEdelta) functions as a prenyl-binding protein. J Biol Chem. 2004;279(1):407–413. [PubMed]
  • Zhao Y, Hong DH, Pawlyk B, et al. The retinitis pigmentosa GTPase regulator (RPGR)-interacting protein: subserving RPGR function and participating in disk morphogenesis. Proc Natl Acad Sci USA. 2003;100(7):3965–3970. [PubMed]
  • Zito I, Downes SM, Patel RJ, et al. RPGR mutation associated with retinitis pigmentosa, impaired hearing, and sinorespiratory infections. J Med Genet. 2003;40(8):609–615. [PMC free article] [PubMed]