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Logo of biotbiofuelBioMed CentralBiomed Central Web Sitesearchsubmit a manuscriptregisterthis articleBiotechnology for Biofuels
 
Biotechnol Biofuels. 2012; 5: 50.
Published online Jul 23, 2012. doi:  10.1186/1754-6834-5-50
PMCID: PMC3463428
Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw
Yubin Zheng,1 Xiaochen Yu,1 Jijiao Zeng,1 and Shulin Chencorresponding author1
1Department of Biological Systems Engineering, L.J. Smith Hall, Washington State University, Pullman, WA, 99164-6120, USA
corresponding authorCorresponding author.
Yubin Zheng: yubin_zheng/at/wsu.edu; Xiaochen Yu: judy_yuxiaochen/at/wsu.edu; Jijiao Zeng: zengjijiao/at/wsu.edu; Shulin Chen: chens/at/wsu.edu
Received April 26, 2012; Accepted July 2, 2012.
Abstract
Background
Lipids produced from filamentous fungi show great promise for biofuel production, but a major limiting factor is the high production cost attributed to feedstock. Lignocellulosic biomass is a suitable feedstock for biofuel production due to its abundance and low value. However, very limited study has been performed on lipid production by culturing oleaginous fungi with lignocellulosic materials. Thus, identification of filamentous fungal strains capable of utilizing lignocellulosic hydrolysates for lipid accumulation is critical to improve the process and reduce the production cost.
Results
The growth performances of eleven filamentous fungi were investigated when cultured on glucose and xylose. Their dry cell weights, lipid contents and fatty acid profiles were determined. Six fungal strains with high lipid contents were selected to culture with the hydrolysate from dilute sulfuric acid pretreatment of wheat straw. The results showed that all the selected fungal strains were able to grow on both detoxified liquid hydrolysate (DLH) and non-detoxified liquid hydrolysate (NDLH). The highest lipid content of 39.4% was obtained by Mortierella isabellina on NDLH. In addition, NDLH with some precipitate could help M. isabellina form pellets with an average diameter of 0.11 mm.
Conclusion
This study demonstrated the possibility of fungal lipid production from lignocellulosic biomass. M. isabellina was the best lipid producer grown on lignocellulosic hydrolysates among the tested filamentous fungi, because it could not only accumulate oils with a high content by directly utilizing NDLH to simplify the fermentation process, but also form proper pellets to benefit the downstream harvesting. Considering the yield and cost, fungal lipids from lignocellulosic biomass are promising alternative sources for biodiesel production.
Keywords: Filamentous fungi, Mortierella isabellina, Microbial lipid, Biodiesel, Lignocellulosic biomass, Wheat straw
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