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Logo of bmcpsBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Plant Biology
 
BMC Plant Biol. 2012; 12: 104.
Published online Jul 9, 2012. doi:  10.1186/1471-2229-12-104
PMCID: PMC3462685
Characterization of a set of novel meiotically-active promoters in Arabidopsis
Junhua Li,1,3 Andrew D Farmer,2 Ingrid E Lindquist,2 Stefanie Dukowic-Schulze,1 Joann Mudge,2 Tao Li,1 Ernest F Retzel,corresponding author2 and Changbin Chencorresponding author1
1Department of Horticultural Science, University of Minnesota, 1970 Folwell Avenue, St. Paul, MN, 55108, USA
2National Center for Genome Resources, 2935 Rodeo Park Drive E, Santa Fe, NM, 87505, USA
3College of Life Sciences, Henan Normal University, Xinxiang, Henan, 453007, China
corresponding authorCorresponding author.
Junhua Li: lijh0909/at/gmail.com; Andrew D Farmer: adf/at/ncgr.org; Ingrid E Lindquist: iel/at/ncgr.org; Stefanie Dukowic-Schulze: sdukowic/at/umn.edu; Joann Mudge: jm/at/ncgr.org; Tao Li: lixx0765/at/umn.edu; Ernest F Retzel: efr/at/ncgr.org; Changbin Chen: chenx481/at/umn.edu
Received March 9, 2012; Accepted June 13, 2012.
Abstract
Background
Homologous recombination, together with selection, laid the foundation for traditional plant breeding. The recombination process that takes place during meiotic cell division is crucial for the creation of novel variations of highly desired traits by breeders. Gaining control over this process is important for molecular breeding to achieve more precise, large-scale and quicker plant improvement. As conventional ubiquitous promoters are neither tissue-specific nor efficient in driving gene expression in meiocytes, promoters with high meiotic activities are potential candidates for manipulating the recombination process. So far, only a few meiotically-active promoters have been reported. Recently developed techniques to profile the transcriptome landscape of isolated meiocytes provided the means to discover promoters from genes that are actively expressed in meiosis.
Results
In a screen for meiotically-active promoters, we examined ten promoter sequences that are associated with novel meiotic candidate genes. Each promoter was tested by expressing a GFP reporter gene in Arabidopsis. Characterization of regulatory regions revealed that these meiotically-active promoters possessed conserved motifs and motif arrangement. Some of the promoters unite optimal properties which are invaluable for meiosis-directed studies such as delivering specific gene expression in early meiosis I and/or meiosis II. Furthermore, the examination of homologs of the corresponding genes within green plants points to a great potential of applying the information from Arabidopsis to other species, especially crop plants.
Conclusions
We identified ten novel meiotically-active promoters; which, along with their homologs, are prime candidates to specifically drive gene expression during meiosis in plants and can thus provide important tools for meiosis study and crop breeding.
Keywords: Meiosis, Homologous recombination, Promoter, GFP, cis-regulatory elements, Plant molecular breeding
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