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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
J Neurosci. Author manuscript; available in PMC 2012 December 27.
Published in final edited form as:
PMCID: PMC3458508

Loss of Intra- and Inter-Network Resting State Functional Connections with Alzheimer’s Disease Progression

Mathew Brier, BS,1,2 Jewell B. Thomas, BA,1 Abraham Z. Snyder, MD, PhD,3,1 Tammie L. Benzinger, MD, PhD,1,3 Dongyang Zhang, PhD,1 Marcus E. Raichle, MD,1,3,4 David M. Holtzman, MD,1,4,5,6 John C. Morris, MD,1,4,6 and Beau M. Ances, MD, PhD1,4,5


Alzheimer’s disease (AD) is the most common cause of dementia. Much is known concerning AD pathophysiology but our understanding of the disease at the systems level remains incomplete. Previous AD research has used resting state functional connectivity magnetic resonance imaging (rs-fcMRI) to assess the integrity of functional networks within the brain. Most studies have focused on the default-mode network (DMN), a primary locus of AD pathology. However, other brain regions are inevitably affected with disease progression. We studied rs-fcMRI in five functionally defined brain networks within a large cohort of human participants of either gender (n=510) that ranged in AD severity from unaffected (clinical dementia rating, CDR 0) to very mild (CDR 0.5) to mild AD (CDR 1). We observed loss of correlations within not only the DMN but other networks at CDR 0.5. Within the salience network (SAL), increases were seen between CDR 0 and CDR 0.5. However, at CDR 1, all networks, including SAL, exhibited reduced correlations. Specific networks were preferentially affected at certain CDR stages. In addition, cross-network relations were consistently lost with increasing AD severity. Our results demonstrate that AD is associated with widespread loss of both intra- and inter-network correlations. These results provide insight into AD pathophysiology and reinforce an integrative view of the brain’s functional organization.

Keywords: Alzheimer’s disease, fMRI, resting state functional connectivity, BOLD, default mode network, salience network


Alzheimer’s disease (AD) is the most common cause of dementia. The pathological hallmarks of AD are accumulation of amyloid beta 1-42 (Aβ1-42) plaques and tau tangles (Blennow et al., 2006; Holtzman et al., 2011). The application of novel neuroimaging techniques may provide additional insight into AD pathophysiology.

Resting-state functional connectivity magnetic resonance imaging (rs-fcMRI) is a method that non-invasively assesses brain function (for review, Zhang and Raichle, 2010). rs-fcMRI measures the temporal correlation of spontaneous fluctuations of the blood oxygen level dependent (BOLD) signal between regions (Biswal et al., 1995). Correlated regions have been reproducibly classified into resting-state networks (RSNs) using both independent components analysis (Calhoun et al., 2001; Beckmann et al., 2005) and seed-based analysis (Damoiseaux et al., 2006; Shehzad et al., 2009). RSNs are of scientific interest because they recapitulate the topographies of task-related functional response (Beckmann et al., 2005; Smith et al., 2009). Importantly, rs-fcMRI eliminates performance confounds associated with task-based functional neuroimaging (Hyvärinen, 1999; Ewers et al., 2011). We employed seed-based analysis, which affords straightforward comparison across groups.

The Default Mode Network (DMN), the RSN most active in the absence of task (Raichle et al., 2001; Macey et al., 2004), was the first network to be identified as affected by AD (Greicius et al., 2004). There is a compelling co-localization between DMN abnormalities and AD histopathology (Aβ1-42 deposition and atrophy) (Braak and Braak, 1991; Buckner et al., 2005; 2008; Bero et al., 2011; Drzezga et al., 2011). Moreover, the DMN is associated with episodic memory (Buckner, 2004; Cole et al., 2010), a cognitive domain impaired early in AD. Following the work of Greicius and colleagues (Greicius et al., 2004), additional resting state studies of AD have reported abnormalities in the DMN (Rombouts et al., 2005; 2005; Sorg et al., 2007; 2007; Hedden et al., 2009; 2009; Zhang et al., 2010; Jones et al., 2011). Further, DMN rs-fcMRI abnormalities increase with disease progression (Supekar et al., 2008; Sanz-Arigita et al., 2010; Zhang et al., 2010; Zhou et al., 2010; Agosta et al., 2011; Petrella et al., 2011).

Focal changes in DMN function occur early in AD. Eventually, however, the entire cortex becomes affected. However, the sequence of involvement of functional systems outside the DMN is not well known. Only a few rs-fcMRI studies have investigated the effects of AD in other RSNs (Wang et al., 2007; Zhou et al., 2008; Murphy et al., 2009; Agosta et al., 2011). rs-fcMRI studies of AD have found decreased graph-based connectivity measures across many RSNs (Supekar et al., 2008; Fox et al., 2009; Sanz-Arigita et al., 2010). Other studies have reported varied changes in rs-fcMRI between regions of interest (ROI) across RSNs (Wang et al., 2007; Agosta et al., 2011).

Here, we report a cross-sectional rs-fcMRI study of AD in a large cohort, assessing five RSNs as a function of disease stage. Our results demonstrate that widespread disturbances in RSNs are seen even in very mild (CDR 0.5) and mild (CDR 1) AD.


Patient characteristics

Data were examined from 559 human participants of either sex enrolled in memory and aging studies at the Knight Alzheimer’s Disease Research Center at Washington University in St Louis. The Washington University in Saint Louis Human Research Protection Office approved all procedures. Following informed consent, each participant had clinical examinations, neuropsychological performance testing, and neuroimaging studies.

Clinical examination

Experienced clinicians conducted semi-structured interviews with the participant and a knowledgeable collateral source. The Clinical Dementia Rating (CDR) was used to determine the presence or absence of dementia and, if present, to stage its severity. CDR 0 indicates cognitive normality while a CDR 0.5 corresponds to very mild AD and CDR 1 specifies mild AD (Morris, 1993; Hagmann et al., 2008). All CDR > 0 participants had a clinical diagnosis of dementia of the Alzheimer’s type in accordance with the National Institute of Neurological and Communicative Disorders and Stroke and the Alzheimer’s Disease and Related Disorders Association (McKhann et al., 1984; Buckner et al., 2009; Sperling et al., 2009; Zuo et al., 2011; 2011).

Image acquisition

Imaging was performed using a 3T Siemens scanner (Erlangen, Germany) equipped with a standard 12-channel head coil. A high-resolution structural scan was acquired using a 3-dimensional sagittal T1-weighted magnetization-prepared rapid gradient echo (MPRAGE; echo time [TE] = 16 msec, repetition time [TR] = 2,400 msec, inversion time [TI] = 1,000 msec, flip angle = 8°, 256 × 256 acquisition matrix, 1 × 1 × 1 mm voxels). The high-resolution MPRAGE was used for definitive atlas registration. High-resolution 2-D multi-slice oblique axial spin density/T2-weighted fast spin echo (FSE) structural images were acquired using slice tilts and positions computed by slice preregistration (TE = 455 msec, TR = 3,200 msec, 256 × 256 acquisition matrix, 1 acquisition, 1 × 1 × 1 mm voxels). These T2-weighted FSE data were used in the rs-fcMRI atlas registration procedure. rs-fcMRI scans were collected using a gradient spin-echo sequence (TE = 27 msec, TR = 384 msec, field of view = 256 mm, flip angle = 90°, 4 mm isotropic voxels) sensitive to the BOLD contrast (T2* weighting). Complete brain coverage was obtained using 36 contiguous slices acquired parallel to the anterior commissure/posterior commissure plane. Two six minute rs-fcMRI runs (164 volumes each) were acquired during which participants were instructed to fixate on a visual cross-hair and not fall asleep

Preprocessing of rs-fcMRI

Initial preprocessing followed conventional methods as previously described (Shulman et al., 2010; Drzezga et al., 2011). Briefly, this included compensation for slice-dependent time shifts, elimination of systematic odd-even slice intensity differences due to interleaved acquisition and rigid body correction for head movement within and across runs. rs-fcMRI data were intensity scaled (one multiplicative factor applied to all voxels of all frames within each run) to obtain a mode value of 1000 (Ojemann et al., 1997; Morris et al., 2001; Fagan et al., 2009). This scaling facilitated assessment of voxel-wise variance for purposes of quality assurance but did not affect computed correlations. Atlas transformation was achieved by composition of affine transforms connecting the rs-fcMRI volumes with the T2W and MPRAGE structural images. Head movement correction was included in a single resampling that generated a volumetric time-series in 3mm3 atlas space.

Additional preprocessing in preparation for correlation mapping included (i) spatial smoothing (6 mm full width half maximum Gaussian blur in each direction), (ii) voxel-wise removal of linear trends over each run, (iii) temporal low-pass filtering that retained frequencies below 0.1 Hz and (iv) reduction of spurious variance by regression of nuisance waveforms derived from head motion correction and extraction of the time-series from regions (of “non-interest”) in white matter and cerebral spinal fluid (CSF). This regression step included the time-series averaged over the whole brain (Buckner et al., 2005; Fox et al., 2009; Bero et al., 2011). A consequence of whole brain signal regression is that all subsequently computed correlations are effectively partial correlations of first order controlling for widely shared variance.

Quality assurance (QA)

Particular care was taken to minimize the impact of head motion both at the individual and at the group levels. For each individual, the preprocessed rs-fcMRI data were passed through a procedure that detected frames (volumes) with excessively high whole brain root mean squared (rms) signal change over time (Sheline et al., 2010; Smyser et al., 2010; Power et al., 2012; Van Dijk et al., 2012). These frames were subsequently excluded from rs-fcMRI assessments. The fraction of frames so excluded was < 10% in each group (no significant effect of group as factor on fraction of frames removed). Overall, 10 individuals had a large proportion of high noise frames (>25% frames identified as contaminated) and were therefore excluded from the analysis. Additional QA measures included rms head displacement (in mm) derived from the motion correction procedure and the voxel-wise time-series standard deviation (s.d.) averaged over the whole brain (de Calignon et al., 2012; Van Dijk et al., 2012). QA-based exclusion criteria were empirically determined with the objective of maximizing the number of included subjects while achieving QA parameter distribution equivalence across groups. Individuals (n=39) with a mean preprocessed rs-fcMRI signal s.d. > 2.5% (after nuisance regression) or rms movement exceeding 1.25 mm were also excluded. The mean rms movement before and after the removal of contaminated frames is shown in Table 1. There were no significant differences among groups in QA parameters (Table 1).

Table 1
The mean (standard deviation) root mean square (RMS) of movement and standard deviation (s.d.) of the signal before and after removal of contaminated frames that contained large amounts of movement. The RMS decreased after this procedure with differences ...

Seed ROI definition

36 spherical (6 mm radius) ROIs, originally representing 7 RSNs, were derived by maximizing the topographic concordance between results obtained by seed-based correlation mapping and by spatial ICA (sICA: (Beckmann et al., 2005; Fagan et al., 2009)). The fastICA algorithm (Hyvärinen, 1999; Greicius et al., 2004; Sestieri et al., 2011) was implemented in MATLAB. rs-fcMRI data were analyzed from 17 healthy young adults acquired in a prior study (Greicius et al., 2004; Fox and Raichle, 2007; Sestieri et al., 2011). Following preprocessing and atlas transformation as described above, but omitting nuisance regression, sICA was run on the rs-fcMRI data concatenated over all runs (4 runs that were 7 minutes in duration) and subjects. Concurrently, correlation mapping was performed using initial seed coordinates taken from multiple sources (Table 2). Loci of matching peaks in the correlation maps and the ICA results were selected as ROI centers. To maximize comparison of certain features of our results compared to prior findings, 4 ROIs within the SAL network were taken directly from the literature (Shulamn et al., 1997; Raichle et al., 2001; Greicius et al., 2004; Seeley et al., 2007; Zhou et al., 2010; 2010; Sestieri et al., 2011). Three originally separate RSNs corresponding to the primary auditory, primary visual and somatomotor cortices were combined into a single sensory-motor network (SMN). Thus, a total of 5 RSN were analyzed and included: DMN; dorsal attention network (DAN); salience (SAL); control (CON); sensory-motor (SMN) (Figure 1).

Figure 1
Schematic representation of the 36 canonical expanded seed regions used for evaluating composite scores for 5 brain resting state networks (RSNs). Abbreviations are as follows. DMN- Default Mode Network, DAN- Dorsal Attention Network, CON- Control Network, ...
Table 2
regions, MNI (Montreal Neurological Institute) coordinates, and sample of references supporting choice of ROIs. Colors indicate network membership as in Figure 1. References included contain ROIs that are within the expanded seed regions of interest (ROI)s ...

rs-fcMRI procedures

Correlation maps were produced by extracting the time course from each of the 36 seed regions. The Pearson correlation coefficient (r) was computed between the time course from a seed region and all other brain voxels. Statistical tests of rs-fcMRI results were computed after application of Fisher’s z transform (z = 0.5ln[(1+r)/(1−r)]). CDR group average images were computed by averaging individual correlation maps across subjects. Group difference significance maps were computed by a random effects analysis of the Fisher z transformed correlation maps (voxel-wise p<0.001, uncorrected; cluster size = 5).

Statistical analysis

To obtain quantitative results at the level of RSN node pairs, the group-averaged Fisher z-transformed correlation maps were sampled in areas surrounding the original seeds. These regions were obtained by thresholding the mean (z-transformed) correlation map averaged over groups (weighting each group equally) at a threshold of |z| = 0.3 and isolating the cluster of voxels surrounding each seed. Results using these “expanded seed ROIs” were qualitatively similar to, but considerably less noisy, than directly computing seed pair correlation matrices. The use of expanded seed ROIs also reduced the variance across individuals. This improvement in quantitative stability is understandable as the spatial extent of group differences in correlation maps is greater than the volume of the original seeds. All presently reported results concerning ROI pairs were obtained by evaluating z-transformed correlation maps within expanded seed ROIs. The values extracted from these expanded seed ROIs were subjected to one-way analysis of variance (ANOVA) (3 levels, CDR 0, 0.5, and 1). The p-values were corrected for the false discovery rate (q=0.05) and significant effects assessed with post-hoc t-tests between the CDR 0 vs. CDR 0.5 and CDR 0.5 vs. CDR 1 contrast levels (p < 0.05).

Inspection of these results suggested that correlations within and across RSNs were progressively lost with advancing AD stage. To perform statistical tests of these effects while avoiding sampling error at the level of node pairs, we computed individual subject composite scores for each network for each CDR stage. Thus, the composite DMN score for a subject k was computed as ckDMN=left angle bracketzijkright angle bracketi,j[set membership]DMN, where i and j refer to a ROI pair and left angle bracket right angle bracket represents the mean across subjects. Similarly, ckX was computed as the average correlation within network X in subject k. A similar strategy was used to define cross-network composite scores. Thus, ckX,Y=left angle bracketzijkright angle bracketi[set membership]X,j[set membership]Y was computed the average correlation between RSNs X and Y in subject k. This approach to statistical inference achieves data reduction and reduces the impact of sampling error across node pairs. On the other hand, this method potentially obscures focal phenomena. To assess statistical significance, the ck of all RSNs and RSN pairs was entered into one-way ANOVAs with CDR status as a factor. The resulting p-values were Bonferroni corrected for 5 (within network) and 10 (between network) multiple comparisons, respectively. Significant effects were assessed by post-hoc t-tests of the CDR 0 vs. CDR 0.5 and CDR 0.5 vs. CDR 1 contrasts (p < 0.05).


Demographics of participants

A total of 510 participants had acceptable neuroimaging studies following QA measures. The average age was 77 years with 47% of the participants being male; 386 were CDR 0, 91 were CDR 0.5, and 33 were CDR 1. No significant differences in age, education, or sex were present among the three groups (all p > 0.33). Mini-mental status exam (MMSE) scores were systematically lower with greater CDR stage (mean MMSE = 29 for CDR 0, mean MMSE = 27 for CDR 0.5, and mean MMSE= 20 for CDR 1)

Functional connectivity of the PCC

Correlation maps generated using a seed in posterior cingulate cortex (PCC) (0, −51, 29) for each of the groups are shown in Figure 2A–C. Correlation magnitude within the DMN and anti-correlations with other RSNs were systematically lower for higher CDR. The CDR 0 - CDR 0.5 comparison (Figure 2D) showed decreases in correlations between the PCC and medial prefrontal cortex (mPFC), inferior temporal cortex (iTMP), and lateral parietal cortex (LP), all nodes of the DMN. Reduced anti-correlations were seen in somatosensory, motor, and premotor regions bilaterally. These results suggest that decreases in functional connectivity with the PCC are detectable at the group level even at the earliest symptomatic disease stage (CDR 0.5). Similar, but more pronounced effects were evident in the CDR 0.5 - CDR 1 comparison (Figure 2E).

Figure 2
Resting state functional connectivity magnetic resonance imaging (rs-fcMRI) maps using the posterior cingulate cortex (PCC) as a seed region projected onto a characteristic brain slice. Correlations (orange and red) and anti-correlations (dark blue) with ...

To more thoroughly investigate the effects of disease severity within the DMN, we assessed correlations between the PCC and other DMN nodes. The z-transformed correlation for each seed-ROI pair was subjected to a one-way ANOVA for effect of CDR (Table 3). Each ANOVA yielded a p value that was FDR corrected at q = 0.05 (critical p = 0.02). Some but not all nodes of the DMN showed significant differences with higher CDR. Within these affected node pairings, several patterns associated with disease severity emerged. Between the PCC and mPFC, a reduction in correlation occurred between CDR 0 and CDR 0.5 but not between CDR 0.5 and CDR 1. This was in contrast to LP regions where correlations were preserved at early stages but decreased at CDR 1. Finally, within iTMP, correlation was systematically reduced at successively higher CDR stages. Correlations between the PCC and other nodes of the DMN (including thalamus and cerebellum) were not significantly affected by CDR stage, although a trend was observed.

Table 3
Effect of CDR status on correlations between the PCC and DMN nodes. Column 2 and 3 show ANOVA results. Columns 4, 6 and 8 show mean z-transformed correlation values. Interleaved columns (5 and 7) show results of t-tests between CDR stages. Bold indicates ...

All RSNs were affected with increasing AD severity

We next investigated the effects of AD severity across a wider set of expanded-seed ROI pairs (Table 2). Each network showed a distinctive pattern (Figure 3). To assess the changes in all ROIs for all networks, 36 x 36 matrices were computed (Figure 4). In Figure, 4 intra-network correlations are arrayed in blocks along the diagonal; cross-network correlations appear in off-diagonal blocks. The CDR 0 matrix (Figure 4A) shows positive correlations within each network and negative correlations between networks. The CDR 0.5 matrix (Figure 4B) and, to an even greater extent, the CDR 1 matrix (Figure 4C), show generally reduced correlations within networks as well as reduced anti-correlations between networks. The effects of higher CDR are displayed as difference matrices in Figures 4D and E. For a majority of ROI pairs, the sign of the correlation difference (earlier stage minus later stage) matches the sign of the correlation itself. Thus, warm hues in Figures 4D and E predominantly indicate reduced positive correlations while cool hues predominantly indicate reduced anti-correlations.

Figure 3
rs-fcMRI maps for each of the four non-DMN networks obtained in the CDR 0, CDR 0.5, and CDR 1 groups. Each row shows the correlation map obtained for a given network using a representative seed ROI. For these maps, the left MT+ was used as a seed region ...
Figure 4
ROI pair correlation matrices for CDR 0 (A), CDR 0.5 (B), and CDR 1 (C). The ROIs are grouped by RSN. Intra-network correlations appear on diagonal bocks; inter-network correlations appear in off diagonal blocks. D, CDR 0 – CDR 0.5 difference ...

Intra-network correlations vs. CDR stage

As assessed by composite scores, all networks showed significant decreases with higher CDR stage, except for the SAL (Figure 5A; Table 4). A slight but significant increase in the composite SAL score was seen between CDR 0 and CDR 0.5. All intra-network composite scores were lower at CDR 1 as compared to CDR 0.5. In particular, the SAL composite score was lower at CDR 1. This decrease was nearly significant when compared to CDR 0 (t(416) = 1.79, p = 0.07).

Figure 5
Mean composite z scores for intra and inter-networks across CDR status. A, Mean composite scores for the five investigated networks. All network composite scores tended towards 0 with increasing disease severity. A transient increase in functional correlations ...
Table 4
Effect of CDR within individual RSNs. Bold indicated significance (p < 0.05, Bonferonni corrected). Same format as Table 2.

Inter-network correlations vs. CDR stage

We next investigated cross-network composite scores across CDR stage (Table 5). A significant effect of CDR status was seen for the DMN-DAN, DMN-SMN, and CON-SMN pairs (Figure 5B). In these three cases, anti-correlations were consistently weaker (trend towards 0) comparing CDR 1 to CDR 0.5 (Table 3). The other seven RSN pairs did not show significant difference between CDR status (Figure 5C, Table 5). Of these, three were anti-correlated and four were positively correlated. It should be noted that, despite increases in SAL correlations at CDR 0.5, no increases were seen in inter-network composite scores involving the SAL at any stage.

Table 5
Effect of CDR on RSN pairs. Bold indicated significance (p < 0.05, Bonferonni corrected). Same format as Table 4.

Intra-network correlations vs. sum of box scores

The CDR is a clinically useful tool but is categorical rather than continuous. A better estimate of decline of resting state functional connectivity with increasing AD severity may be obtained by comparing composite scores using the sum of boxes (CDR-SB) (Morris et al., 2001). We performed an ANCOVA on CDR-SB vs. RSN composite scores. In this analysis, we only included participants with a CDR-SB > 0 (i.e., only CDR 0.5 and CDR 1 subjects). We did not include CDR-SB = 0 subjects (i.e., CDR 0 subjects) as the large number of individuals within this group would impose a floor effect and violate the assumptions of most statistical tests. We modeled the direct effect of CDR-SB (i.e., the correlation between composite scores and CDR-SB across networks), the main effect of network (i.e., the difference in absolute magnitude differences in composite scores across different RSNs), and the interaction between network and CDR-SB (differences in the slope of the CDR-SB vs. composite score relationship across networks) (Figure 6). The composite scores generally were significantly correlated with CDR-SB within RSNs (F(1,595) = 34, p < 0.0001); however, the slopes did not significantly vary across networks (F(4,595) = 1.39, p = 0.24).

Figure 6
Scatter plots of mean composite z scores vs. CDR-SB. Linear fit lines are shown. Both p and r values for each correlation are shown.

Analysis at the level of ROI pairs

Composite scores (Figure 5) achieve data reduction but do not address potentially focal effects at the individual ROI pair level. To examine this question, we plotted correlation difference vs. correlation mean (across CDRs) for all ROI pairs (Figure 7). In this representation, lower positive correlation between ROI pairs in the more advanced CDR group appears in the upper right quadrant while lower magnitude anti-correlation between ROI pairs (trend towards 0) appears in the lower left quadrant. Similarly, greater positive correlations appear in the lower right quadrant and greater magnitude anti-correlations appear in the upper left quadrant. In the CDR 0.5 vs. CDR 0 comparison (Figure 7A), systematically greater correlations were seen for ROI pairs within the SAL (blue) and DAN (red). This result is consistent with the RSN composite score analysis (Figure 5A). The CDR 0.5 vs. CDR 1 plot (Figure 7B) demonstrates no convincing evidence of greater correlation magnitude in any RSN at more advanced disease stage.

Figure 7
Scatter plots of CDR stage-dependent correlation difference vs. correlation mean. A, CDR 0 and CDR 0.5. B, CDR 0.5 and CDR 1. DAN=red, SAL=blue, other RSNs=black. The lower right quadrant shows positively correlated ROI pairs that are more correlated ...

The points in Figure 7 cluster around a central tendency, but considerable variability is evident. To better understand this variability, we assessed CDR 0 vs. CDR 0.5 and CDR 0.5 vs. CDR 1 differences in individual ROI pairs within RSNs (Figure 8). Figure 8 represents the same data in the same manner as Figure 7 except that the ROI pairs in each of the two comparisons are connected, and only within-network effects are plotted. The majority of trajectories are vertical and to the left. The vertical component of the trajectory indicates that the difference between CDR 0.5 and CDR 1 is larger than the difference between CDR 0 and CDR 0.5. The horizontal component indicates that the mean correlation value decreases with increasing CDR. Further, the trajectories of the individual ROI pairs parallel the trajectory of the composite scores. These results suggest that, within RSNs, the effect of higher CDR stage manifests approximately uniformly over ROI pairs.

Figure 8
CDR stage-dependent correlation difference vs. correlation mean. The plot axes are the same as in Figure 7 but ROI pairs are evaluated at CDR 0 and CDR 0.5 (hollow symbols) and at CDR 0.5 and CDR 1 (solid symbols) and connected by lines. Network composite ...


Our principal finding is that cross-sectionally measured differences in intra-network correlations were overwhelmingly in the direction of loss with advancing disease stage. Within the SAL, transient increases were observed between CDR 0 and CDR 0.5 but these effects were overtaken by significant losses at mild AD (CDR 1) (Figures 4--6).6). Loss of cross-network correlations was also observed predominantly in RSN pairs that were anti-correlated at CDR 0. These effects were overwhelmingly in the direction of reduced anti-correlation (movement to zero) with advancing CDR stage. Intra as well as inter-network correlations declined at different rates. These results suggest that AD is characterized by widespread loss of RSN integrity throughout the brain, occurring in a particular spatial and temporal sequence. It has been suggested that the effects of AD may manifest focally in certain ROI pairs but not others. Our results suggest that functional connectivity changes approximately uniformly across ROI pairs within networks (Figures 7 & 8). Hence, composite scores capture the critical phenomenology.

To date, this is the largest rs-fcMRI study to assess the effects of AD severity on functional connectivity. Only participants with a probable diagnosis of AD were included in CDR > 0 groups. The large sample size enabled us to distinguish between three groups (CDR 0 vs. CDR 0.5 and CDR 0.5 vs. CDR 1). Stringent QA reduced the impact of head motion on rs-fcMRI results.

Our technique included global signal regression during preprocessing (Shulamn et al., 1997; Raichle et al., 2001; Greicius et al., 2004; Seeley et al., 2007; Fox et al., 2009; Zhou et al., 2010; Sestieri et al., 2011). Algebraically, this is approximately equivalent to computing partial correlations treating the global signal as a nuisance regressor (Raichle et al., 2001; Alexander, 2002; Seeley et al., 2007; Hampson et al., 2010). Global signal regression effectively reduces non-neuronal physiological noise (Raichle et al., 2001; Alexander, 2002; Macey et al., 2004; Seeley et al., 2007). Concomitantly, negative correlations are markedly enhanced (de Zubicaray et al., 2001; Raichle et al., 2001; Dosenbach et al., 2007; Seeley et al., 2007; Fox et al., 2009). The merits of this strategy have been debated (see (Raichle et al., 2001; Johnson et al., 2002; Dosenbach et al., 2007; Seeley et al., 2007; Cole et al., 2010) for discussion) but it is generally acknowledged that global signal regression enhances the spatial specificity of obtained results (Raichle et al., 2001; Johnson et al., 2002; Dosenbach et al., 2007; Seeley et al., 2007; Fox et al., 2009; Weissenbacher et al., 2009).

Loss of BOLD signal correlations within the DMN, as found here, is a well-established finding in AD (Casey et al., 1998; Greicius et al., 2004; Rombouts et al., 2005; Fox et al., 2006; Sorg et al., 2007; Sylvester et al., 2007; Hedden et al., 2009). The principal novelty of our work is in extending these observations to other RSNs at two levels of disease severity. For each of five RSNs, we demonstrate reduced intra-network correlations at CDR stage 1. Intra-network effects were RSN dependent. Most RSNs showed significant decreases in composite scores, relative to CDR 0, even at CDR 0.5, except for the DAN, which remained relatively spared, and the SAL, which showed a significant increase. These results are generally consistent with several other recent studies (Casey et al., 1998; Fox et al., 2006; Sylvester et al., 2007; Supekar et al., 2008; Sanz-Arigita et al., 2010; Zhou et al., 2010; Agosta et al., 2011).

Zhou and colleagues reported greater functional connectivity in the SAL of participants with mild dementia (primarily CDR 1) relative to controls (Büchel et al., 1998; Mazoyer et al., 2001; Fox et al., 2006; Dosenbach et al., 2007; Sylvester et al., 2009; Zhou et al., 2010). This discrepancy with respect to the present results may be related to the following differences in methodology. First, Zhou and colleagues performed an ICA analysis while this study used a seed-based approach. Second, a smaller sample was studied (n=12 for CDR > 0 vs. the present n=124). Third, our studies applied greater stringency in the application of rs-fcMRI QA inclusion criteria.

Our work also demonstrates reduced anti-correlations in three specific inter-network relationships (DMN-DAN, DMN-SMN, CON-SMN). All three network pairs showed strong anti-correlations within CDR 0 subjects, which became progressively attenuated with increasing CDR. In particular, the DMN-DAN pair showed a nearly significant difference in the magnitude of the anti-correlations between CDR 0 and CDR 0.5, but all of these RSN pairs showed a significantly weaker anti-correlations at CDR 1 compared to CDR 0.5. We believe that these effects are not an artifact of signal processing. While it is true that whole brain signal regression mandates that, for every decreasing positive correlation, there be increases in negative correlations (Büchel et al., 1998; Mazoyer et al., 2001; Fox et al., 2006; Dosenbach et al., 2007; Murphy et al., 2009; Sylvester et al., 2009), the topography of these differences is not constrained (for an extended discussion see (Fox et al., 2009)).

Why these three network pairs were preferentially impacted in AD remains a question. We hypothesize that this result relates to the high level of anti-correlation at CDR 0 (Figures 4C & 5B-C). Indeed, the three most highly anti-correlated RSN pairs demonstrated the greatest reduction in negative correlation with increasing AD severity. This result suggests that these anti-correlations reflect relationships predisposed to loss of functional connectivity via as yet not understood mechanism(s). Two of the three involved RSN pairs are members of the DMN, which is known to be a connectivity hub, both anatomically (Hagmann et al., 2008) and functionally (Buckner et al., 2009; Sperling et al., 2009; Zuo et al., 2011; 2011). Our results suggest that if one RSN of a RSN pair is abnormal, that dysfunction may then spread and cause synaptic dysfunction within the unaffected RSN of the pair (Drzezga et al., 2011).

Aβ is an early marker of AD, with the deposition of amyloid plaques and possibly oligomers playing an early and pivotal role in synaptic dysfunction. In addition, tau aggregation, which independently occurs during normal aging and in the early stages of AD, is further accelerated by the presence of concomitant amyloid pathology (Morris et al., 2001; Fagan et al., 2009). Furthermore, Aβ1-42 deposition preferentially occurs within the DMN (Buckner et al., 2005; Bero et al., 2011) and has been correlated with changes in rs-fcMRI (Sheline et al., 2010). The mechanism whereby pathology spreads from the DMN to other RSNs remains a question. One recently advanced hypothesis is based on the finding that misfolded tau can spread trans-synaptically through neural networks where it can induce tau misfolding in the post-synaptic neuron (de Calignon et al., 2012).

Another possibility is suggested by the present results. Proceeding with caution, as we are considering a fundamentally cellular-molecular question on the basis rs-fcMRI, we first note that loss of functional connectivity within the DMN is one of the earliest findings in AD (Greicius et al., 2004; for recent review see Mevel et al., 2011). We suggest that disordered neural communication may be a mechanism whereby AD pathology spreads from the DMN to the rest of the brain. Accordingly, loss of anti-correlations should be evident in the CDR 0 vs. CDR 0.5 contrast, as presently observed (Figures 2D, ,4D4D and and5,5, Table 3). This model also is generally consistent with observed non-uniformity over space and CDR stage. Moreover, the model predicts that spread of pathology is self-reinforcing. This is consistent with the presently observed acceleration of change (Figures 4 and and5).5). This essentially informational view of pathology spread is entirely compatible with molecular mechanisms involving amyloid or tau or both (Fagan et al., 2009).

This study has limitations as follows: First, all presented data are cross sectional in nature. Additional longitudinal studies are needed to assess the progression of disease in the same participants. Longitudinal studies would enable investigation of which network properties predict AD progression. Second, our results reflect the particular selection of seeds chosen for investigation. Some details in the presently reported results might be different with another selection of a seeds or different analysis strategy (e.g., dual-regression (Zuo et al., 2010)). The set of ROIs used in our analyses represents a good faith effort to survey five major RSNs in a principled manner while keeping the total number manageable in order to minimize the multiple comparisons burden. Third, our sampling of participants with more advanced AD is relatively limited. Finally, we did not perform partial volume correction of rs-fcMRI data; consequently, possible effect of regional atrophy on our results cannot be excluded.


Funding support came from Medical Scientist Training Program Grant to WUSTL (MRB) (5T32GM007200-37), Knight Alzheimer’s Disease Research Center (ADRC) Pilot Grant (3255 ADRC 26) (BMA), National Institute of Mental Health (NIMH) (K23MH081786) (BMA), National Institute of Nursing Research (NINR) (R01NR012907 and R01NR012657) (BMA), Dana Foundation (DF10052) (BMA), Alene and Meyer Kopolov Fund for Geriatric Psychiatry and Neurology (BMA), National Institute of Aging (NIA) (P01AG026276, P01AG026276, P01AG03991 and P50 AG05681) (JCM), National Institute of Neurological Disorders and Stroke (NS06833) (MER, AZS), Washington University Center for Translational Neuroscience (P30NS057105) (DMH), NIMH P30NS048056 (AZS), and ARRS Foundation (TB). The authors would also like to thank Russ Hornbeck for his invaluable assistance with the figures in this manuscript and the Clinical Core of the ADRC for participant assessments. Mr. Brier, Mr. Thomas, Dr. Zhang, Dr. Snyder and Dr. Raichle, report no disclosures. Dr. Ances serves on the advisory board to Lily and Medscape and participates in a clinical trial of anti-dementia drugs for Pfizer. Dr. Benzinger consults for Biomedical Systems, Inc. and ICON Medical Imaging and receives research support from Avid Radiopharmaceuticals. Dr. Holtzman reports consulting for Pfizer, Bristol-Myers Squibb, and Innogenetics and is on the scientific advisory boards of En Vivo, Satori, and C2N Diagnostics. Dr. Morris is currently participating in clinical trials of antidementia drugs sponsored by Janssen Alzheimer Immunotherapy, Eli Lilly and Company, and Pfizer. He reports consulting for AstraZeneca, Bristol-Myers Squibb, Eisai, Elan/Janssen Alzheimer Immunotherapy Program, Genentech, Lilly, Merck, Novartis, Otsuka Pharmaceuticals, Pfizer/Wyeth, and Schering Plough.


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