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Ayu. 2012 Jan-Mar; 33(1): 136–142.
PMCID: PMC3456852

Pharmaceutical standardization of Jala Shukti Bhasma and Mukta Shukti Bhasma

Abstract

Shukti is an important component of Sudha Varga, which is considered as the latest class in the field of Rasa Shastra. Two types of Shukti have been mentioned in Rasa Shastra texts i.e. Jala Shukti and Mukta Shukti according to the availability. In present study, an attempt has been made to develop a standard manufacturing procedure (SMP) of Jala Shukti Bhasma and Mukta Shukti Bhasma. Five batches of Jala Shukti Bhasma and Mukta Shukti Bhasma were prepared and standardization was attempted by maintaining batch manufacturing records of individual batches. During pharmaceutical procedures like Shodhana, Bhavana, Marana, etc. due care of temperature, its duration, percentage of weight gain or loss and the cost factor of the end product, etc. were considered. The average weight loss observed was 12.08 g i.e. 2.42% and 14.62 g i.e. 2.92% during Jala Shukti and Mukta Shukiti Shodhana respectively. Average weight loss found was 38.94 g i.e. 7.79% in Jala Shukti Bhasma while in Mukta Shukti Bhasma, it was 35.24 g i.e. 7.05%. At the end of the pharmaceutical procedure, it was found that Mukta Shukti Bhasma is 2.8 times costlier than Jala Shukti Bhasma.

Keywords: Bhasma, Jala Shukti, Marana, Mukta Shukti, Shodhana

Introduction

Ancient Acharyas of Rasa Shastra had included Shukti in different Vargas like Shukla Varga,[1] Shodhaniya Gana,[2] Shweta Varga,[3] Uparasa,[4] Uparatna,[5] Shankhadi Vigyaniyam[6] etc. but later on in the 20th century A.D., it was included under Sudha Varga due to the predominance of calcium or Sudha. First time the author of Ayurved Prakasha[7] has mentioned two types of Shukti (Jala Shukti and Mukta Shukti) along with their synonyms, Shodhana and therapeutic uses accepted these in detail. Almost all authors of Rasa Shastra have mentioned two types of Shukti. In Rasa Tarangini, Shukti is described in detail under the broad heading of Shankhadi Vigyaniyam. Further two types of Mukta Shukti i.e. broad and circular and ear-like (Karnikakara) according to their shape. Among those, Karnikakara Mukta Shukti is the best variety and should be used in medicine. If it is not available, the first variety can be used as the substitute of the second variety but it is of lower quality.[8]

There are some controversies regarding Jala Shukti and Mukta Shukti. Jala Shukti is easily available and cheaper, while Mukta Shukti is not easily available and costlier than Jala Shukti. Jala Shukti is mostly procured from fresh water, while Mukta Shukti is procured from sea water. In this attempt, Jala Shukti Bhasma and Mukta Shukti Bhasma were prepared in order to develop a Standard Manufacturing Procedure (SMP).

Materials and Methods

Collection of raw materials

Jala Shukti was collected from Gomati river bank near Jaunpur, Uttar Pradesh. Mukta Shukti was procured from Prabhas Patan near Veraval, Gujarat. Nimbu (Citrus medica (Linn.) Burm. F) was purchased from the local market of Jamnagar. Kumari (Aloe vera Tourn. ex Linn.) was collected from the botanical garden of Gujarat Ayurved University, Jamnagar [Figures [Figures1,1, ,22].

Figure 1
Ashuddha Jala Shukti
Figure 2
Ashuddha Mukta Shukti

Shodhana of Ashuddha Jala Shukti and Mukta Shukti:

For Shodhana of both Shukti, 500 g was taken in each batch and Nimbu Swarasa was used as media for Swedana. Nimbu Swarasa was prepared as per the Sharangadhara Samhita.[9] Before processing it was washed with potable water properly, then cut in two pieces with knife. The cut part of the Nimbu was put in an in extractor and compressed to collect Swarasa which was filtered through a cotton cloth. The details of observations and results are presented in Table 1. The Shodhana process of both Shukti was carried out as per the Rasatarangini.[10] Details of ingredients and their ratio used in the Shodhana process have been shown in Tables Tables22 and and3.3. Ashuddha Jala Shukti was made into small pieces, kept in two folded white clean cotton cloth and Pottali was prepared [Figure 3]. It was suspended in Dola Yantra containing 3 L of Nimbu Swarasa (lemon juice) and mild heat was applied to boil Nimbu Swarasa which was maintained for 3 h. When the level of Nimbu Swarasa decreased, again extra 1 L (average) was added. After completion of processing, heating was stopped and left for self-cooling. Then the Pottali was opened and Shukti pieces were washed thoroughly with hot water and dried. Similar procedure was followed for Mukta Shukti Shodhana. The observation and results obtained during the Shodhana process have been presented in Tables Tables44,,55 and Figures Figures44,,55.

Table 1
Quantity of Nimbu Swarasa obtained
Table 2
Ingredients and their quantity for Jala Shukti Shodhana
Table 3
Ingredients and their quantity for Mukta Shukti Shodhana
Table 4
Results obtained during the process of Jala Shukti Shodhana
Table 5
Results obtained during Mukta Shukti Shodhana
Figure 3
Shodhana by Dola Yantra
Figure 4
Shuddha Jala Shukti
Figure 5
Shudhha Mukta Shukti

Marana of Shuddha Jala Shukti and Mukta Shukti

First Puta

Shoddhita materials (Shuddha Jala Shukti or Mukta Shukti) were kept in Sharava in one layer and Sharava Samputa was prepared. After that, Sharava Samputa was allowed to dry in sunlight. The Sharava Samputa was subjected to Puta Paka in the conventional Puta i.e. Gaja Puta. After placing ignited cow dung cakes and filling two-thirds of the pit with 60 cow dung cakes, Sharava Samputa was kept on them and the remaining one-third part was filled with 40 cow dung cakes to cover Sharava Samputa. After complete burning of all the cow dung cakes, the pit was allowed to self-cool. On the next day, after self-cooling of Sharava Samputa, it was opened and Jala Shukti pieces - Mukta Shukti pieces were collected carefully and weighed.[11] The results obtained during the first Puta have been presented in Tables Tables66,,77 and Figure 6.

Table 6
Results obtained during the first Puta of Jala Shukti Bhasma
Table 7
Results obtained during the first Puta of Mukta Shukti Bhasma
Figure 6
Shukti after 1st Puta

Second Puta

Kumari Swarasa was extracted by Nishpidana (expression) Vidhi.[9] Leaves of Kumari were washed in tap water; thorny ridges and apex were cut by knife. Mucilaginous pulp was separated from the leaves with the help of knife and pulp was churned in mixer and then strained through cotton cloth. The results are presented in Table 8.

Table 8
Result obtained during processing of Kumari Swarasa

Jala Shukti powder - Mukta Shukti powder (obtained after 1st Puta) was levigated with Kumari Swarasa in porcelain mortar until it formed a thick paste [Figures [Figures7,7, ,8]8] and become suitable for making Chakrikas (pellets). Small amount of levigated doughy mass was made into flat and round-shaped Chakrikas. The prepared pellets were kept on a plastic sheet for drying.

Figure 7
Bhavana procedure of Jala Shukti powder with Kumari Swarasa after 1st Puta
Figure 8
Bhavana procedure of Mukta Shukti powder with Kumari Swarasa after 1st Puta

After proper drying of Chakrikas, they were weighed and kept in an earthen Sharava. That Sharava was covered by another earthen Sharava and the junction between the two Sharavas was sealed by a cotton cloth smeared with Mulatani Mitti and again allowed to dry completely. The remaining procedure was carried out the was similar to first Puta. The observations and results obtained during the second Puta have been presented in Tables Tables99,,1010 and Figures Figures9912. The final product was powdered and sieved through 120 # mesh to obtain fine Bhasma. Then Jala Shukti Bhasma or Mukta Shukti Bhasma was filled inside ‘0’ no. (500 mg in volume) gelatine capsules for oral administration.

Table 9
Results obtained during the second Puta of Jala Shukti Bhasma
Table 10
Results obtained during the second Puta of Mukta Shukti Bhasma
Figure 9
Jala Shukti Chakrikas after 2nd Puta
Figure 12
Mukta Shukti Bhasma
Figure 10
Mukta Shukti Chakrikas after 2nd Puta
Figure 11
Jala Shukti Bhasma

Average temperature pattern

The temperature of Puta during Marana procedure of Jala Shukti and Mukta Shukti was recorded at regular intervals of 15 min for 6 h. During Jala Shukti Bhasma preparation, the average peak temperature observed was 859.80°C and 852.6°C in the first and second Puta respectively, whereas in Mukta Shukti Bhasma preparation the peak temperature was found to be 850.80°C and 849.20°C in the first and second Puta respectively. The temperature pattern observed during the first Puta and second Puta of both Bhasma are presented in Graphs Graphs11 and and22.

Graph 1
Average temperature pattern observed during Jala Shukti Bhasma preparation (Temp. in °C at Y axis, time in hours at X axis)
Graph 2
Average temperature pattern observed during Mukta Shukti Bhasma preparation (Temp. in °C at Y axis, time in hours at X axis)

Discussion

For the Shodhana procedure, each batch of Jala Shukti and Mukta Shukti, 500 g were taken and average four l of Nimbu Swarasa was required for each batch to complete Swedana process. The ratio of materials and liquid media required was 1:8.

Average 4 L Nimbu Swarasa was obtained from 8 kg of Nimbu. During Shodhana procedure, it was observed that the color of media i.e. Nimbu Swarasa changed from yellow to turbid yellow and more viscous which may be due to the reaction between the media and the substance. The temperature of the media was maintained between 105°C and 110°C throughout the procedure. A whitish powder-like substance was deposited at the bottom of the Dola Yantra suggesting the escape of impurities through the pores of the Pottali. After Shodhana, it was noticed that Jala Shukti pieces became whiter with shining and became smooth while Mukta Shukti pieces became bright white with rainbow-like shining and smoothness.

The average weight loss observed was 12.08 g i.e. 2.42% and 14.62 g i.e. 2.92% in Jala Shukti and Mukta Shukti Shodhana respectively [Tables [Tables44,,5].5]. It may be due to removal of impurities that dissolved and escaped through the pores of Pottali during Shodhana procedure. Some particles may be lost during washing with hot water after Shodhana.

For the first Puta, a fixed amount of cow dung cakes (100 in number) was taken for average 487.92 g of Jala Shukti and 485.38 g of Mukta Shukti. Average weight of 100 Upalas was found to be 12.62 kg. After the first Puta, Jala Shukti and Mukta Shukti pieces became pale white in color, brittle and soft. Shining was disappeared but fragility attributed after 1st Puta. The average weight loss observed was 26.26 g i.e. 5.25% after the first Puta in the preparation of Jala Shukti Bhasma and 27.44 g i.e. 5.49% in the preparation of Mukta Shukti Bhasma. After that Jala Shukti and Mukta Shukti pieces were powdered well and taken for further procedure i.e. Bhavana.

Jala Shukti powder and Mukta Shukti powder were levigated with Kumari Swarasa in porcelain mortar with the help of pestle until it formed a thick paste and was suitable for making Chakrikas (pellets). Total 250 ml of Kumari Swarasa was used for levigation and was done continuously for 3 h. Extra Kumari Swarasa was added from time to time for maintaining proper levigation. Levigation was done properly with uniform and sufficient pressure to make the materials fine. The pellets were made uniform in shape and size for proper heat exposure. After drying, the average weight of one Chakrika was observed 8 to 10 g, the average diameter was 2.0 to 2.5 cm and thickness was 0.5 to 0.7 cm.

For the second Puta, fixed amount of cow dung cakes (100 in number) were taken for average 473.74 g of Jala Shukti and 472.56 g of Mukta Shukti. Average weight of 100 Upalas was found to be 12.89 kg. After the second Puta, Chakrikas of Jala Shukti Bhasma were found to be soft, fragile, smooth and white in color while Chakrikas of Mukta Shukti Bhasma were found to be more smooth and bright white in color. After powdering of the Jala Shukti Bhasma became white whereas Mukta Shukti Bhasma became bright white [Table 11]. Both Bhasma became fine, soft and smooth and they had passed classical parameters like Rekhapurnatwa, Varitaratwa, Gatarasatwa, Sukshmatwa, Mridutwa [Table 12]. Regarding Sudha Varga, no specific parameters are described in the classics of Rasa Shastra for Bhasma Pariksha except the color of Bhasma. Rasa Tarangini has mentioned the color of Shukti Bhasma as ‘Himakundendu Samkasham’ i.e. white which was observed in both Bhasma after the second Puta.

Table 11
Classical physical parameters of both Bhasma
Table 12
Classical chemical parameters of both Bhasma

The average weight loss was found to be 38.94 g i.e. 7.79% in Jala Shukti Bhasma after the second Puta while in the Mukta Shukti Bhasma it was observed to be 35.24 g i.e. 7.05%. This loss may be due to vaporization of water and burning of some organic or inorganic materials. The peculiar odor observed during levigation with Kumari Swarasa disappeared after the second Puta in both Bhasma.

Varitaratwa was attempted to evaluate in both the samples of Shukti Bhasma by following the classical method as explained in Rasa Ratna Samuchaya. In general, this test is not mentioned in classical literature, though the authors tried to evaluate it. The Varitaratwa test was observed to be positive in Mukta Shukti Bhasma, while it was mild in Jala Shukti Bhasma, it may be due to some difference regarding its inorganic chemical elements. Rekhapurnatwa was observed in both Bhasma after the second Puta indicating fineness of Bhasma.

At the end of the pharmaceutical procedure, it was found that Mukta Shukti Bhasma is 2.8 times costlier than Jala Shukti Bhasma.

Conclusion

  1. 51.75% Nimbu Swarasa (average 4.142 l) acquired from 8 kg lemon is sufficient for 500 g of Jala Shukti or Mukta Shukti Shodhana by Swedana Vidhi i.e. ratio of materials and liquid is 1: 8.
  2. To convert 500 g batches of Shukti into Bhasma, minimum 100 cow dung cakes in the first and second Puta were required and peak temperature observed for Jala Shukti was 859.8°C and for Mukta Shukti was 850.8°C.
  3. There is no significant color difference between Jala Shukti Bhasma and Mukta Shuikti Bhasma i.e. white and bright white respectively. Varitaratwa Bhasma Pariksha was found positive in both the Bhasma.

References

1. Bhairabhava Mathan. 4th ed. 40. Vol. 5. Varanasi: Chaukhamba Sanskrit Series; 2005. Rasarnava with Rasa Chandrika Hindi Commentary; p. 62.
2. Kulkarni DA, editor. 3rd ed. 70. Vol. 10. Varanasi: Meherchand Lachmandass; 1982. Rasa Vagbhata, Rasa Ratna Samuchchaya; p. 195.
3. Siddha Nityanath. Rasa Ratnakara Riddhi Khanda. In: Mishra S, editor. 2nd ed. 6. Vol. 2. Varanasi: Chaukhamba Orientalia; 2003. p. 13.
4. Bhairavoktam, Anandakandah . “Siddhiprada” In: Mishra Siddhi Nandan., Prof., editor. Kriyakarn. 1st ed. 7. Vol. 2. Varanasi: Chaukhamba Orientalia; 2008. p. 64.
5. Mishra Brahmashankara., editor. Dhatwadi Varga. 10th ed. 1st Part. Vol. 189. Varanasi: Chaukhamba Sanskrit Sansthan; 2002. Bhava Mishra, Bhava Prakasha with Vidyotini; p. 628.
6. Sharma S. Rasa Tarangini. In: Shastry Kashinatha., editor. 11th ed. 1. Vol. 12. Delhi: Motilal Banarasi Das; 2004. p. 285. Reprint.
7. Mishra Gulrajsharma., editor. Reprint. 326. Vol. 2. Varanasi: Chaukhambha Bharati Academy; 1999. Upadhyaya Madhava, Ayurveda Prakasha, Arthavidyotini and Arthaprakshini; p. 336.
8. Shastry Bramhanand., editor. Dhatwadi Varga. 9th edition. Vol. 189. Varanasi: Chaukhamba Samskrit Samsthana; 1999. Bhava Mishra, Bhava Prakasha with Vidyotini Commentary; p. 628.
9. Shastry Parashuram, vidyasagar, editors. Madhyama Khanda. 6th ed. 2. Vol. 1. Varanasi: Chaukhambha Orientalia; 2005. Sharangadhara, Sharangadhara Samhita with Adhamalla's Gudartha Dipika; p. 137.
10. Sharma S. Rasa Tarangini. In: Shastry Kashinatha., editor. 11th ed. 66. Vol. 12. Delhi: Motilal Banarasi Das; 2004. p. 285. Reprint.
11. Ibid. 12(67):296.

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