Search tips
Search criteria 


Logo of ijclbiowww.springer.comThis journalToc AlertsSubmit OnlineOpen Choice
Indian J Clin Biochem. 2000 July; 15(2): 76–82.
PMCID: PMC3453967

Direct diagnosis ofMycobacterium tuberculosis in blood samples of HIV infected patients by polymerase chain reaction


We have developed a simple, economical and reproducible method for processing blood samples from HIV infected patients for diagnosis of tuberculosis. The procedure was validated on 55 samples selected for tuberculosis based on clinical criteria. 52 patients had radiological changes indicative of pulmonary tuberculosis of which only 28 were positive for AFB in sputum (sensitivity 54%) and 27 for tuberculin (sensitivity 52%). 26 HIV positive patients who showed positive X-ray did not react to tuberculin. The genus PCR probe missed 3 samples (sensitivity 94%) compared to X-ray.M.tuberculosis was detected in the blood of all X-ray positive cases by PCR using TB400 probe (sensitivity 100%) and another probe forM. tuberculosis, IS6110, missed 6 of them (sensitivity 88% compared to X-ray and 89% compared to TB400). It is proposed that this simple sample processing method could be used to screen all blood samples quickly for mycobacteremia using the genus PCR and only those positive for mycobacteria need to be tested forM.tuberculosis. This would save the scarce resources and time by reducing significantly the number of samples to be screened for species confirmation.

Key words: Polymerase Chain Reaction, mycobacteremia, tuberculosis diagnosis in AIDS, DNA probes, Mycobacterium tuberculosis

Full Text

The Full Text of this article is available as a PDF (726K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
1. Stefan H.E., Kanframann J.D.A., emden Tuberculosis: a neglected disease strikes back. Trends in Microbiol. 1993;1:2–5. doi: 10.1016/0966-842X(93)90015-J. [PubMed] [Cross Ref]
2. Kramer F.T., Modilevski A.R., Waliany T.M., Leedom, Barnes P.F. Delayed diagnosis of tuberculosis in-patients with immuno deficiency virus infection. Amer. J. Med. 1990;89:451–456. doi: 10.1016/0002-9343(90)90375-N. [PubMed] [Cross Ref]
3. Daniel T.M. The Rapid Diagnosis of Tuberculosis. A selected review. J. Lab. Clin. Med. 1990;116:277–288. [PubMed]
4. Schluger N.W., Condos R., Lewis S., Rom W.N. Amplification of DNA ofMycobacterium tuberculosis from peripheral blood of patients with pulmonary tuberculosis. Lancet. 1990;344:232–233. doi: 10.1016/S0140-6736(94)92999-8. [PubMed] [Cross Ref]
5. Dewitt D., Steyn L., Shoemaker S., Sogin M. Direct Detection ofMycobacterium tuberculosis in ciinical specimens by DNA amplification. J. Clin. Microbiol. 1990;28:2437–2441. [PMC free article] [PubMed]
6. Eissenach K.D., Stifford M.D., Cave M.D., Bates J.H., Crawford J.T. Detection ofMycobacterium tuberculosis in sputum samples using a polymerase chain reaction. Amer. Rev. Respir. Dis. 1991;144:1160–63. [PubMed]
7. Hance A.J., Grandchamp B., Levy Frebault V., Lecosier D., Rauzier J., Bocart D, Giquel B. Detection of mycobacteria by amplification of mycobacterial DNA. Mol. Microbiol. 1989;3:843–849. doi: 10.1111/j.1365-2958.1989.tb00233.x. [PubMed] [Cross Ref]
8. Hermans P.W.M., Schuitema A.R.J., Sooligen Specific detection ofMycobacterium tuberculosis complex strains by polymerase chain reaction. J. Clin. Microbiol. 1990;28:1204–1213. [PMC free article] [PubMed]
9. Pao C.C., Yen T.S.B., You J.B., Fiss J.S., Chang Detection and identification ofMycobacterium tuberculosis by DNA amplification. J. Clin. Microbiol. 1990;28:1877–1880. [PMC free article] [PubMed]
10. Sjobring U., Mecklenburg M., Andersen A. B., Miorner H. Polymerase Chain Reaction for the detection ofMycobacterium tuberculosis. J. Clin. Microbiol. 1990;28:2200–2204. [PMC free article] [PubMed]
11. Sritharan V, Barker R.H. A simple method for the diagnosing ofM.tuberculosis infection in clinical samples using PCR. Mol. and Cell. Probes. 1991;5:385–395. doi: 10.1016/S0890-8508(06)80011-3. [PubMed] [Cross Ref]
12. Fujiwara H., Kleinholz M.E., Wallis R.S., Ellner J.J. Expression of Functional interleukin 2 receptors by peripheral blood monocytes from patients with active pulmonary tuberculosis. J. Clin. Invest. 1990;85:1777–1784. doi: 10.1172/JCI114635. [PMC free article] [PubMed] [Cross Ref]
13. Toosi Z., Sedor J.R., Lapurga J.P., Ondash R.J., Ellner J.J. Expression of functional interleukin-2 by peripheral blood monocytes from patients with active pulmonary tuberculosis. J. Clin. Invest. 1990;85:1777–84. doi: 10.1172/JCI114635. [PMC free article] [PubMed] [Cross Ref]
14. Mattar S., Morta M., Drobonic L. Sepsis caused byMycobacterium tuberculosis inpatients with AIDS. Enfermedates Infec. Microbiol. Clin. 1992;10:29–31. [PubMed]
15. Sritharan V., Iralu J.V., Barker R.H. Comparison of Genus and Species specific probes for PCR detection of mycobacterial infection. Mol. and Cell. Probes. 1994;8:409–416. doi: 10.1006/mcpr.1994.1058. [PubMed] [Cross Ref]
16. Yuen L.K.W., Ross B.C., Jackson K.M., Dwyer B. Characterisation ofM.tuberculosis strains from Vietnamese patients by Southern blot hybridization. J. Clin. Microbiol. 1993;31:1615–1618. [PMC free article] [PubMed]

Articles from Indian Journal of Clinical Biochemistry are provided here courtesy of Springer