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Indian J Clin Biochem. 2008 October; 23(4): 337–340.
Published online 2008 December 20. doi:  10.1007/s12291-008-0074-7
PMCID: PMC3453139

Oxidative stress and disturbance in antioxidant balance in beta thalassemia major


Repeated blood transfusion in beta thalassemia major patients may lead to peroxidative tissue injury by secondary iron overload. In the present study, 72 children with beta thalassemia major were included. Serum levels of total lipid peroxides, Iron, Total Iron Binding Capacity, Copper, Zinc, Vitamin E, plasma Total Antioxidant Capacity, activity of Erythrocyte Superoxide Dismutase, were measured. The findings were compared with 72 age matched healthy controls irrespective of sex. A significant increase in the levels of lipid peroxide and Iron (p<0.001), whereas, significant decrease in the levels of vitamin-E, Total Antioxidant Capacity and Total Iron Binding Capacity (p<0.001) was observed. Serum Zinc was significantly increased (p<0.001) with significant decrease in the levels of copper (p<0.001). Non Significant increase in the activity of Erythrocyte Superoxide Dismutase (p>0.05) was found in the patients when compared with controls. This suggest that oxidative stress and reduced antioxidant defense mechanism play an important role in pathogenesis of beta thalassemia major.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
1. Widad N.M., Al-Naama L. Meaad. Trace element in patients with beta thalassemia major. Haem. 2003;6(3):376–383.
2. Das N., Chowdhury T.D., Chattopadhyay A. Datta. Attenuation of oxidation stress - induced changes in thalassemic erythrocytes by Vitamin E. Pol J Pharmocol. 2004;56:85–96. [PubMed]
3. Ambekar S.S., Phadke M.A., Balpande D.N., Mokashi G.D., Khedkar V.A., et al. The prevalence and Heterogeneity of Beta Thalassemia Mutation in The Western Maharashtra Population: A hospital based study. IJHG. 2001;1(3):219–223.
4. Livrea M.A., Tesoriere L., Pintaudi A.M., Calabrese A., Maggio A., Freisleben H.J., et al. Oxidative stress and Antioxidant status in beta thalassemia major: Iron overload and depletion of lipid — soluble antioxidants. Blood. 1996;88:3608–3614. [PubMed]
5. Baumgartener T.G. Vitamins. In: Way C.W., editor. Nutrition secrets. Philadephia: Hanley and Belfus; 1999. pp. 13–20.
6. Satoh K. Serum Lipid Peroxide in cerebrovascular diseases, determined by a new colorimetric method. Clin Chim Acta. 1978;90:37–43. doi: 10.1016/0009-8981(78)90081-5. [PubMed] [Cross Ref]
7. Varley H. Ramasay’s Dipyridyl method for iron. In: Practical Clinical Biochemistry: 1967, 4th Edn.: 472.
8. Varley H. Ramasays Dipyridyl method for total iron binding capacity. In: Practical Clinical Biochemistry: 1967; 4th Edn.: 475.
9. Das K. A modified spectrophotometric assay of superoxide dismutase using nitrate formation by superoxide radicals. Ind J Biochem Biophy. 2000;57:201–204.
10. Baker and Frank. Determination of serum tocopherol by colorimetric method. In: Varley’s practical clinical biochemistry, Heinmann Professional Publishing 1988; 6th Ed.902.
11. Iris F.F., Bernin J. S. The Ferric reducing ability of plasma [FRAP] as a measure of “Antioxidant power”. The FRAP Assay. Analytical Biochemistry. 1996;239:70–76. doi: 10.1006/abio.1996.0292. [PubMed] [Cross Ref]

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