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J Headache Pain. Aug 2008; 9(4): 221–224.
Published online Jul 2, 2008. doi:  10.1007/s10194-008-0047-5
PMCID: PMC3451944
Low urinary 6-sulphatoxymelatonin concentrations in acute migraine
Marcelo Rodrigues Masruha,1,2 Domingos Sávio de Souza Vieira,2 Thais Soares Cianciarullo Minett,1 José Cipolla-Neto,3 Eliova Zukerman,2 Luiz Celso Pereira Vilanova,1 and Mario Fernando Prieto Perescorresponding author1,2
1Department of Neurology and Neurosurgery, Federal University of São Paulo, São Paulo, Brazil
2Instituto Israelita de Ensino e Pesquisa Albert Einstein, Hospital Israelita Albert Einstein, Av. Albert Einstein, 627/701, Morumbi, São Paulo, 05651-901 Brazil
3Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
Mario Fernando Prieto Peres, Phone: +55-11-37470456, marioperes/at/
corresponding authorCorresponding author.
Received April 27, 2008; Accepted June 3, 2008.
Substantial evidence points to melatonin as playing a role in the regulation of circadian rhythms, sleep, and headache disorders. The objective of the study was to assess 6-sulphatoxymelatonin (aMT6s) levels in a large consecutive series of patients with migraine, comparing with controls. A total of 220 subjects were evaluated—146 had migraine and 74 were control subjects. Urinary samples were collected into the same plastic container since 8:00 p.m. to 8:00 a.m. of the next day (12-h period) and aMT6s was measured with quantitative ELISA technique. Among patients with migraine, 53% presented pain on the day of the urine samples collection. Their urinary aMT6s concentration was significantly lower than in the urine of patients without pain [14.0 ± 7.3 vs. 49.4 ± 19.0; t(143) = −15.1; 95% CI = −40.0 to −30.8; P < 0.001]. There was no significant difference in the aMT6s concentration of patients with migraine without pain on the day of their urine samples collection and controls [49.4 ± 19.0 vs. 42.5 ± 27.9; t(140) = 1.7; 95% CI = −1.2 to 14.8; P = 0.094]. To our knowledge, this is the first study to demonstrate reduction in melatonin levels during attacks in episodic and chronic migraine.
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Selected References
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