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Cytotechnology. 2006 June; 51(2): 45–50.
Published online 2006 August 3. doi:  10.1007/s10616-006-9013-8
PMCID: PMC3449683

Identity tests: determination of cell line cross-contamination

Abstract

Cell line cross-contamination is a phenomenon that arises as a result of the continuous cell line culture. It has been estimated that around 20% of the cell lines are misidentified, therefore it is necessary to carry out quality control tests for the detection of this issue. Since cell line cross-contamination discovery, different methods have been applied, such as isoenzyme analysis for inter-species cross-contamination; HLA typing, and DNA fingerprinting using short tandem repeat and a variable number of tandem repeat for intra-species cross-contamination. The cell banks in this sense represent the organizations responsible for guaranteeing the authenticity of cell lines for future research and clinical uses.

Acknowledgements

We thank Ms. Angela Barnie for checking the use of English in the manuscript.

Abbreviations

AFLP
Amplified fragment length polymorphism
MCB
Master cell bank
STR
Short tandem repeat
SBT
Sequence based typing
SSO
Sequence specific oligonucleotide
SSOP
Sequence specific oligonucleotide probe
VNTR
Variable number tandem repeat
WCB
Working cell bank

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