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Aquat Biosyst. 2012; 8: 17.
Published online Jul 27, 2012. doi:  10.1186/2046-9063-8-17
PMCID: PMC3445841
Disruption of Yarrowia lipolytica biofilms by rhamnolipid biosurfactant
Devendra H Dusane,1,3 Sushovan Dam,1 Yarlagadda V Nancharaiah,2 Ameeta Ravi Kumar,1 Vayalam P Venugopalan,2 and Smita S Zinjardecorresponding author1
1Institute of Bioinformatics and Biotechnology, University of Pune, Pune, 411 007, India
2Biofouling and Biofilm Processes Section, BARC Facilities, Kalpakkam, 603 102, India
3Present address: Biocolloids and Surfaces Laboratory, Department of Chemical engineering, McGill University, Montreal, QC, Canada
corresponding authorCorresponding author.
Devendra H Dusane: devendra.dusane/at/mail.mcgill.ca; Sushovan Dam: dam.sushovan/at/gmail.com; Yarlagadda V Nancharaiah: yvn/at/igcar.gov.in; Ameeta Ravi Kumar: ameeta/at/unipune.ac.in; Vayalam P Venugopalan: vpv/at/igcar.gov.in; Smita S Zinjarde: smita/at/unipune.ac.in
Received May 29, 2012; Accepted July 27, 2012.
Abstract
Background
Yarrowia lipolytica is an ascomycetous dimorphic fungus that exhibits biofilm mode of growth. Earlier work has shown that biosurfactants such as rhamnolipids are efficient dispersants of bacterial biofilms. However, their effectiveness against fungal biofilms (particularly Y. lipolytica) has not been investigated. The aim of this study was to determine the effect of rhamnolipid on a biofilm forming strain of Y. lipolytica. Two chemical surfactants, cetyl-trimethyl ammonium bromide (CTAB) and sodium dodecyl sulphate (SDS) were used as controls for comparison.
Results
The methylene blue dye exclusion assay indicated an increase in fungal cell permeability after rhamnolipid treatment. Microtiter plate assay showed that the surfactant coating decreased Y. lipolytica biofilm formation by 50%. Rhamnolipid treatment disrupted pre-formed biofilms in a more effective manner than the other two surfactants. Confocal laser scanning microscopic studies showed that biofilm formation onto glass surfaces was decreased by 67% after sub-minimum inhibitory concentration (sub-MIC) treatment with rhamnolipids. The disruption of biofilms after rhamnolipid treatment was significant (P<0.05) when compared to SDS and CTAB.
Conclusion
The results indicate a potential application of the biological surfactant to disrupt Y. lipolytica biofilms.
Keywords: Biofilm, Biosurfactant, CTAB, Rhamnolipid, SDS, Yarrowia lipolytica
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