In this study, 84 sessions of workout had a meaningful impact on body weight and body mass index. Interestingly, body weight is something that most researchers expect to be changed during body-building exercises; however, according to the analysis of studies, the quality of body weight changes and how it happens during body-building workout is different 
In this research, It seems that one of the main reasons in losing body weight and body fat mass is the calorie limitation and the change in the length of the track used by runners. In addition to that, the change in body weight could be caused by a change in runners’ psychological state 
According to this research IgA intensity stayed the same before and after the preparation period, while it was 5.75% during competition period, which was not significant.
Regardless of the fact that IgA is responsible for membranous immunity and much of that is available in human saliva, IgM and IgG play an important role in humoral immunity 
. Immunoglobulin G is one of the most important serum immunoglobulins and its smooth, natural function is of great weight for human immune system, and it also protects our bodies from infections and other germ activities 
According to this research, 84 sessions of exercise during preparation time did not have a significant meaning on serum IgG in athletes’ rest time, Hence, this level has reduce during competition period, even so, it was not significant. Imanipour et al, in their study with active men, during 14 weeks found no significant difference between pre and posttests in IgG 
. Verde et al reported that IgG concentration in ten elite male runners that participated in increased training schedules by an average of 38% for 3 wk, decreased significantly
The current study results about immunoglobulin M show that preparation period exercises brought about 27.66% decrease in IgM, during competition period IgM reduced more than before the preparation period, while it was not significant.
Mckune and colleagues reported a significant decrease by 23 percent in IgM intensity after a 24-hour full marathon 
. Coffman and colleagues confirmed IgM reduction after the marathon 
. There is this possibility that the changes in serum immunoglobulins depend on the intensity and the time of exercise.
Overall, the researchers have concluded that the changes in immunoglobulin intensity depend on several factors; thus, they suggested different mechanisms to justify the Ig intensity changes a few of which we will mention here 
Ig level has to be calculated while examining plasma volume changes. The slight increase in Ig intensity (20 percent), which happens after vigorous exercises, is somehow related to plasma volume changes. The less than 10 percent increase in Ig intensity is usually caused by the daily changes or exchanges of outer veins, lymph vessels, or the blood circulation. Therefore, the decreasing 27, 23, 20 to 35 percent changes in of IgM volume in this study and other studies cannot be based on plasma volume changes [1, 7, 23]
Although most of the contrasting studies indicate that immunoglobulin level and its natural amount in resting athletes do not differ from that of non-athletes, the Ig intensity and antibody production decreased in those runners and athletes who practiced intense and competitive training 
According to this research, cortisol levels significantly decrease during preparation phase; although, it increased during pre-competition phase. testosterone levels intangibility increased and decreased respectively during preparation and pre-competition phases. T/C ratio increases significantly during preparation phase and it decreased during pre-competition phase.
Thus, these findings correspond with findings from Lehmann and colleagues (1992), who reported the decrease in cortisol without variation in testosterone after 3 weeks by an increase in the volume and intensity during training in endurance and semi-endurance runners 
. Daly and colleagues (2005) after studying the effect of prolonged endurance exercise reported a significant reduction in cortisol in athletes 
. There have been several reasons for changes in intensity of cortisol in body after physical activities, including hypothalamus stimulation, Pituitary, Adrenal (HPA), adrenocorticotropic release, central body temperature, PH changes, sympathetic nervous system, hypoxia, and ethacridine lactate gathering. Vigorous body activity with 60 to 65 percent of oxygen consumption stimulates HPA axis and increases core temperature; it also causes an increase in releasing cortisol and freeing them from carrying proteins. The increase in pituitary response during vigorous exercises has a big impact on androgenic anabolic. 
According to the conducted research, the amount of cortisol release is different during days and the most amounts are released during mornings, and the closer we get to the afternoons the less the amounts of that is seen, except in stress periods in which its level does not increase 
, Since the time of collecting samples was in the afternoon and the subjects were in the recess time without having any stress, these factors might have influenced the release of cortisol slightly. Adaptation index to exercises in males is used through considering the ratio of free testosterone to cortisol and the ratio of testosterone to cortisol 
An increase in the ratio of free testosterone to cortisol is mainly caused by a decrease in serum cortisol levels. A decrease of cortisol after exercise is probably due to an increase in cortisol circulation removal, or adrenocorticotropic hormone decrease 
. In the period before the competitions, the release of anabolic and catabolic hormones has been reverted in a way that cortisol levels increased more than preparation period. At this period, the ratio of serum testosterone and testosterone to cortisol ratio had decreased, which could indicate an increase in the catabolic procedure of runners’ bodies before the competitions. The result of the research shows that whenever T/C ratio decreases more than 30 percent and this situation continues to prolong, it can lead to overtraining for the athletes