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AMB Express. 2012; 2: 37.
Published online Jul 29, 2012. doi:  10.1186/2191-0855-2-37
PMCID: PMC3444338
Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources
Hironobu Morisaka,1 Kazuma Matsui,1 Yohei Tatsukami,1 Kouichi Kuroda,1 Hideo Miyake,2,3,4 Yutaka Tamaru,2,3,4 and Mitsuyoshi Uedacorresponding author1
1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, 606-8502, Japan
2Department of Life Science, Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie, 514-8507, Japan
3Department of Bioinformatics, Life Science Reseach, Mie University, 1577, Kurimamachiya, Tsu, Mie, 514-8507, Japan
4Laboratory of Applied Biotechnology, Venture Business Laboratory, Mie University, 1577 Kurimamachiya, Tsu, Mie, 514-8507, Japan
corresponding authorCorresponding author.
Hironobu Morisaka: miueda/at/kais.kyoto-u.ac.jp; Kazuma Matsui: kazuma/at/kais.kyoto-u.ac.jp; Yohei Tatsukami: tatsukami.youhei.88a/at/st.kyoto-u.ac.jp; Kouichi Kuroda: k_kuro/at/kais.kyoto-u.ac.jp; Hideo Miyake: miyake/at/bio.mie-u.ac.jp; Yutaka Tamaru: ytamaru/at/bio.mie-u.ac.jp; Mitsuyoshi Ueda: miueda/at/kais.kyoto-u.ac.jp
Received May 18, 2012; Accepted July 20, 2012.
Abstract
We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901–902]. Our system employed a long monolithic column (300 cm), which provides better performance and higher resolution than conventional systems. Twenty-three cellulosomal proteins were, without purification, identified by direct analysis of the culture medium. Proteome analysis of the C. cellulovorans cellulosome after culture in various carbon sources demonstrated the production of carbon source-adapted cellulosome components.
Keywords: Clostridium cellulovorans, Cellulosome, Focused proteome analysis, Monolithic column
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