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From:
Published online Sep 13, 2012. doi: 10.1371/journal.pone.0044766
Table 2
Primers used in this study.
NameNucleotide SequenceApplications
Rex-FwForward, 5′-GGTCGTGCTCTCCTGAATTATCG-3′RT-PCR analysis
GuaA-RvReverse, 5′-TTCGTGATTCAAGCAGCAGTTC-3′RT-PCR analysis
148FwForward, 5′-ATTTTTTTGTGAAAAGGGTTACAATGACAAG-3′Rex-binding site #1 oligo
148RvReverse, 5′-CTTGTCATTGTAACCCTTTTCACAAAAAAAT-3′Rex-binding site #1 oligo
m148FwForward, 5′-ATTTTTAACAGTAAAGGGTTACAATGACAAG-3′Mutation of Rex-binding site #1 oligo
m148RvReverse, 5′-CTTGTCATTGTAACCCTTTACTGTTAAAAAT-3′Mutation of Rex-binding site #1 oligo
140FwForward, 5′-TTATGATGTCATTATCATCG-3′20 bp oligo within gshR promoter
140RevReverse, 5′-CGATGATAATGACATCATAA-3′20 bp oligo within gshR promoter
1363cFwForward, 5′-GATGATTTTTCGTCTATA-3′18 bp oligo within tpn promoter
1363cRevReverse, 5′-TATAGACGAAAAATCATC-3′18 bp oligo within tpn promoter
p1053FwForward, 5′-GGCAAAGTAAAGTCTAACAGTGTTTA-3′rex promoter amplification
p1053RevReverse, 5′-TTATCGAAAGTCACTGTACTTCCTC-3′rex promoter amplification
p148FwForward, 5′-CCTTGGTACTTTATTGCTGATAAGAGT-3′adhE promoter amplification
p148RevReverse, 5′-TAGCTGCTTCTTCAGCAGTGAG-3′adhE promoter amplification
p1410FwForward, 5′-ACTAAGCAAACCTCCCATCTA-3′frdC promoter amplification
p1410RevReverse, 5′-ATGACAGTTCCTCGCATTT-3′frdC promoter amplification
p1363FwForward, 5′-GGTCTGGCATCAGATGG-3′tpn promoter amplification
p1363RevReverse, 5′-AGTCCTTAAGTAAAACCACATC-3′tpn promoter amplification
p140FwForward, 5′-ACAAGCCCATACTGAGTTTG-3′gshR promoter amplification
p140RevReverse, 5′-GGCTAGAGTCACACCTGAAACA-3′gshR promoter amplification
p1115FwForward, 5′-TCTGGAAGAGCCCGAGC-3′ldh promoter amplification
p1115RevReverse, 5′-CAGCATCCGCACAGTCT-3′ldh promoter amplification
1054 55′Forward, 5′-AGTTTAATACAGAGATTGGAGAG-3′PCR of region 5′ to guaA for mutagenesis
1054 53′RIReverse, 5′-TGATATCGGGAATTCTAGCATGTG-3′PCR of region 5′ to guaA for mutagenesis
1054 35′RIForward, 5′-TCTGAATTCACTTTTCTAGGTGTTC-3′PCR of region 3′ to guaA for mutagenesis
1053 53′ BmReverse, 5′-TCTAACTGTCGCTGGATCCATACC-3′PCR for rex::guaA deletion/mutation
1054 35′ HdForward, 5′-TCTAAGCTTACTTTTCTAGGTGTTC-3′PCR for rex::guaA deletion/mutation
1053comp-5RIForward, 5′-ACAGAGAATTCTGGACTTAGAGTTGTC-3′PCR of rex for complementation
1053comp-3BmReverse, 5′-AGTCACTGTGGATCCTCCTTTTTC-3′PCR of rex for complementation
1054comp-5BmForward, 5′-AAATAAGGAGGATCCATGAATAAACC-3′PCR of guaA for complementation
1054comp-3HdReverse, 5′-ATAAGCTTCCTTCAGGTTCAAC-3′PCR of guaA for complementation
5′-1053Forward, 5′-ATGGATCCATCCCTAAGGCGACAATC-3′For Rex expression
3′-1053Reverse, 5′-TGACATAAGCTTATGGTGTATAAGAC-3′For Rex expression
Biotin-adhE1 FwForward, 5′-Biotin-TTTTGTGAAAAGGGTTACAATG-3′“Hot” Oligo for Chemiluminescent EMSA
adhE1 FwForward, 5′-TTTTGTGAAAAGGGTTACAATG-3′“Cold” Oligo for Chemiluminescent EMSA
adhE1 RevReverse, 5′-CATTGTAACCCTTTTCACAAAA -3′“Cold” Oligo for Chemiluminescent EMSA
1053 55′Forward, 5′-TCTAATGAAGATGCTCTTCCTC-3′PCR for rex::guaA deletion & complementation
1054 33′Reverse, 5′-TCTTATGTATTCAATCCATTCTGC-3′PCR for rex::guaA deletion, complementation,
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