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From:
Published online Sep 13, 2012. doi: 10.1371/journal.pone.0044766
Table 1
Bacterial strains and plasmids used in this study.
Strains/plasmidsMajor propertiesReferences
S. mutans UA159wildtype, serotype c[17]
S. mutans TW239UA159 derivative, Δrex, Kanr[25]
S. mutans TW239CTW239 carrying rex in pDL278, Kanr, Sp[25]
S. mutans TW263UA159 derivative, ΔguaA, KanRThis study
S. mutans TW263CTW263 carrying guaA in pDL278, Kanr, SprThis study
S. mutans JB314UA159 derivative, Δrex, ΔguaA, SprThis study
S. mutans JB315JB314 complemented with guaA, Δrex, Kanr, SprThis study
S. mutans JB316JB314 complemented with rex, ΔguaA, Kanr, SprThis study
E. coli DH10BCloning host, ΔmcrA, ΔmcrBC, Δmrr, and ΔhsdInvitrogen
E. coli M15Expression strain, carrying pRep4, KanrQiagen, Inc.
pDL278Shuttle vector, Spr[33]
pQE30Expression vector, AmpRQiagen, Inc.
Note: Kanr, Spr, and Ampr are kanamycin, spectinomycin and ampicillin resistant, respectively.
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