rRex binding with and without the inclusion of NAD+ and NADH.
(A) rRex interactions with the promoter of gshR in the presence of 10 mM NAD+ or 10 mM NADH. The binding affinity between rRex and the gshR promoter is enhanced in the presence of NAD+ while the presence of NADH inhibits complex formation. (B) rRex interactions with the promoter of frdC in the presence of 10 mM NAD+ or 10 mM NADH. At low concentrations, rRex binds better to the repressor site of frdC when compared to gshR (A). Inclusion of NAD+ did not change the mobility pattern; however, NADH causes the complex to dissociate.
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