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Logo of bmcmicrBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Microbiology
 
BMC Microbiol. 2012; 12: 116.
Published online Jun 21, 2012. doi:  10.1186/1471-2180-12-116
PMCID: PMC3438084
Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates
Ben Fan,1,2 Lilia C Carvalhais,2 Anke Becker,3 Dmitri Fedoseyenko,4 Nicolaus von Wirén,4 and Rainer Borrisscorresponding author2,5
1Institute of Forest Protection, Nanjing Forestry University, Longpan Road 159, 210037, Nanjing, China
2Institut für Biologie Bakteriengenetik, Humboldt Universität Berlin, Chausseestrasse 117, D-10115, Berlin, Germany
3Molekulare Genetik, Institut für Biologie III, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104, Freiburg, Germany
4Leibniz Institute for Plant Genetics and Crop Plant Research, Corrensstr. 3, 06466, Gatersleben, Germany
5ABiTEP GmbH, Glienicker Weg 185, D-12489, Berlin, Germany
corresponding authorCorresponding author.
Ben Fan: fanben2000/at/gmail.com; Lilia C Carvalhais: l.carvalhais/at/uq.edu.au; Anke Becker: anke.becker/at/biologie.uni-freiburg.de; Dmitri Fedoseyenko: fedoseyenko/at/yahoo.ie; Nicolaus von Wirén: vonwiren/at/ipk-gatersleben.de; Rainer Borriss: rainer.borriss/at/rz.hu-berlin.de
Received April 1, 2012; Accepted May 31, 2012.
Abstract
Background
Plant root exudates have been shown to play an important role in mediating interactions between plant growth-promoting rhizobacteria (PGPR) and their host plants. Most investigations were performed on Gram-negative rhizobacteria, while much less is known about Gram-positive rhizobacteria. To elucidate early responses of PGPR to root exudates, we investigated changes in the transcriptome of a Gram-positive PGPR to plant root exudates.
Results
Bacillus amyloliquefaciens FZB42 is a well-studied Gram-positive PGPR. To obtain a comprehensive overview of FZB42 gene expression in response to maize root exudates, microarray experiments were performed. A total of 302 genes representing 8.2% of the FZB42 transcriptome showed significantly altered expression levels in the presence of root exudates. The majority of the genes (261) was up-regulated after incubation of FZB42 with root exudates, whereas only 41 genes were down-regulated. Several groups of the genes which were strongly induced by the root exudates are involved in metabolic pathways relating to nutrient utilization, bacterial chemotaxis and motility, and non-ribosomal synthesis of antimicrobial peptides and polyketides.
Conclusions
Here we present a transcriptome analysis of the root-colonizing bacterium Bacillus amyloliquefaciens FZB42 in response to maize root exudates. The 302 genes identified as being differentially transcribed are proposed to be involved in interactions of Gram-positive bacteria with plants.
Articles from BMC Microbiology are provided here courtesy of
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