The purpose of the present study was to conduct direct measurements in a large sample of dried femori in order to record certain morphometric parameters of the femoral condyles and determine whether there are gender and side differences. Three hundred sixty (Greek) Caucasian dried femori (180 left and 180 right), from 192 males and 168 females, were measured using a digital caliper. The mean age was 67.52 years. The mean bicondylar width of the femur was 8.86cm ± 0.42cm in men and 7.85cm ± 0.30cm in women (P < 0.01). The relative values for the medial condylar depth were 6.11cm ± 0.34cm and 5.59cm ± 0.29cm (P < 0.05); for the lateral condylar depth were 6.11cm ± 0.33cm and 5.54cm ± 0.21cm (P < 0.01); for the intercondylar width were 2.20cm ± 0.18cm and 1.87cm ± 0.10cm (P < 0.001); for the intercondylar depth were 2.78cm ± 0.16cm and 2.37cm ± 0.12cm (P < 0.001). No significant side-to-side difference was observed in any parameter. The femoral condyles differences in anatomy between genders might be useful to the design of total knee prostheses. The contralateral healthy side can be safely used for preoperative templating since there were no significant side differences.