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Logo of jbcThe Journal of Biological Chemistry
 
J Biol Chem. 2012 August 17; 287(34): 28503–28507.
Published online 2012 July 9. doi:  10.1074/jbc.C112.387514
PMCID: PMC3436563

The Autophagy-related Protein Kinase Atg1 Interacts with the Ubiquitin-like Protein Atg8 via the Atg8 Family Interacting Motif to Facilitate Autophagosome Formation*An external file that holds a picture, illustration, etc.
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Abstract

In autophagy, a cup-shaped membrane called the isolation membrane is formed, expanded, and sealed to complete a double membrane-bound vesicle called the autophagosome that encapsulates cellular constituents to be transported to and degraded in the lysosome/vacuole. The formation of the autophagosome requires autophagy-related (Atg) proteins. Atg8 is a ubiquitin-like protein that localizes to the isolation membrane; a subpopulation of this protein remains inside the autophagosome and is transported to the lysosome/vacuole. In the budding yeast Saccharomyces cerevisiae, Atg1 is a serine/threonine kinase that functions in the initial step of autophagosome formation and is also efficiently transported to the vacuole via autophagy. Here, we explore the mechanism and significance of this autophagic transport of Atg1. In selective types of autophagy, receptor proteins recognize degradation targets and also interact with Atg8, via the Atg8 family interacting motif (AIM), to link the targets to the isolation membrane. We find that Atg1 contains an AIM and directly interacts with Atg8. Mutations in the AIM disrupt this interaction and abolish vacuolar transport of Atg1. These results suggest that Atg1 associates with the isolation membrane by binding to Atg8, resulting in its incorporation into the autophagosome. We also show that mutations in the Atg1 AIM cause a significant defect in autophagy, without affecting the functions of Atg1 implicated in triggering autophagosome formation. We propose that in addition to its essential function in the initial stage, Atg1 also associates with the isolation membrane to promote its maturation into the autophagosome.

Keywords: Autophagy, Membrane Trafficking, Protein Degradation, Protein-Protein Interactions, Yeast, AIM, GABARAP, LC3, LIR, ULK1

Introduction

Autophagy (macroautophagy) is a pathway for membrane transport to the lysosome/vacuole (1). During this process, a cup-shaped membrane called the isolation membrane is formed and expanded into a double membrane-bound vesicle called the autophagosome. The autophagosome eventually fuses with those lytic organelles, allowing degradation of its contents. Under starvation conditions, autophagosomes randomly sequester portions of the cytoplasm. In contrast, autophagosomes also selectively engulf degradation targets, such as cytotoxic protein aggregates, and damaged or surplus organelles (2, 3). These processes require autophagy-related (Atg)2 proteins. The core Atgs mediate the formation of the autophagosomal membrane, whereas receptor and adaptor Atgs recognize degradation targets and recruit the core Atgs to allow membrane formation along the surfaces of targets. The ubiquitin-like protein Atg8 (microtubule-associated protein 1 light chain 3 (LC3) or gamma-aminobutyric acid A receptor-associated protein (GABARAP) in mammals) is conjugated to phosphatidylethanolamine (PE) and thereby anchored to the isolation membrane, where it is involved in the expansion of the membrane (47). A proportion of Atg8 is retained inside the complete autophagosomes and is transported to the lysosome/vacuole. In addition, Atg8 also plays an important role in the incorporation of degradation targets into autophagosomes; it interacts with receptor proteins that contain the Atg8 family interacting motif (AIM) (or the LC3 interacting region), which binds to highly conserved, hydrophobic pockets in Atg8 (2, 3, 8, 9). Atg8-PE conjugates on the isolation membrane may link the target-receptor complex to the membrane to facilitate its engulfment.

Atg1 (unc-51-like kinase 1 (ULK1) in mammals) is a serine/threonine kinase essential for autophagy. In yeast, Atg1 forms a complex with the regulatory proteins Atg13, Atg17, Atg29, and Atg31 in response to autophagy-inducing signals (10, 11). This complex serves as a scaffold to organize the pre-autophagosomal structure (PAS), a dynamic assembly of Atg proteins, during the initiation of autophagosome formation (1, 1113). The formation of the PAS-scaffolding complex also enhances the kinase activity of Atg1 (10), which regulates the PAS localization of downstream Atgs, probably via phosphorylation of some of those proteins.

In a previous study, we found an intriguing behavior of Atg1 during autophagy (12). Although Atg1 has been implicated in the early stage of autophagosome formation, a fraction of this kinase is incorporated into the autophagosome and transported to the vacuole. In addition, although the PAS localization of Atg1 requires complex formation with the regulatory proteins, only Atg1 among these proteins is significantly transported to the vacuole.

In this study, we investigated the mechanism of the incorporation of Atg1 into the autophagosome. We found that Atg1 contains an AIM, with which it directly binds to Atg8. Mutations that affected this interaction accordingly reduced the vacuolar transport of Atg1. Thus, interaction with Atg8 allows Atg1 to associate with the isolation membrane, resulting in Atg1 incorporation into the autophagosome. We also found that mutations in the AIM of Atg1 attenuated autophagic activity, although they did not affect Atg1 functions involved in the initiation of autophagosome formation. These results suggest that Atg1 on the isolation membrane plays an important role in the promotion of autophagosome formation, distinct from its role in the initial stage.

EXPERIMENTAL PROCEDURES

Yeast Strains, Plasmids, and Media

The yeast strains and oligonucleotides used in this study are listed in supplemental Tables S1 and S2, respectively. Construction of these strains and plasmids and the compositions of culture media are described in supplemental Methods.

Fluorescence Microscopy

Fluorescence microscopy was performed as described previously (14).

Immunoblotting

Immunoblotting analysis was performed as described previously (14). Monoclonal antibodies against GFP (Roche Applied Science) was used for detection of GFP-fused proteins. Antibodies against Atg8 (anti-Atg8-2), Atg1 (anti-Atg1), Atg13 (anti-Atg13), and Atg17 (anti-GST-Atg17-1) were described previously (10, 11, 14).

Yeast Two-hybrid Assay

The yeast two-hybrid assay was performed using Matchmaker Gal4 Two-hybrid System 3 (Clontech). The AH109 strain was transformed with different combinations of pGADT7-ATG1 and pGBD-C-ATG8 plasmids and their vectors, and the overnight cultures were spotted onto SC-LT (control), SC-LTH (−His), and SC-LTA (−Ade) agar plates followed by incubation at 30 °C for 1, 2, and 3 days, respectively.

Immunoprecipitation

Yeast cells expressing Atg1-GFP and 3×FLAG-Atg8 were grown to mid-log phase, treated with rapamycin for 2 h, and disrupted in HSE buffer (25 mm HEPES-KOH (pH 7.2), 750 mm sorbitol, 5 mm EDTA) containing 0.5× Complete protease inhibitor mixture (Roche Applied Science) using Multi-beads shocker and 0.5-mm YZB zirconia beads (Yasui Kikai). Sodium chloride and Triton X-100 were added to the lysates to concentrations of 50 mm and 0.5%, respectively. A monoclonal anti-FLAG M2 antibody (Sigma) and Dynabeads protein G (Invitrogen) were incubated with the lysates at 4 °C for 3 h. After washing the beads, the bound proteins were eluted by incubating the beads in SDS sample buffer at 65 °C for 5 min. To examine the formation of the PAS-scaffolding complex, the cell lysates were prepared from rapamycin-treated yeast cells expressing Atg1-GFP by essentially the same method as described above, except that TSG buffer (50 mm Tris-HCl (pH 8.0), 150 mm NaCl, 10% glycerol) was used instead of HSE buffer. The lysates were treated with 0.2% n-dodecyl-β-d-maltoside at 4 °C for 30 min and incubated with GFP-Trap_M beads (ChromoTek) at 4 °C for 2 h. After washing the beads, the bound proteins were eluted as described above.

ALP Assay

To quantify autophagic activity of yeast cells, an alkaline phosphatase (ALP) assay was performed as described previously (15).

RESULTS

Atg1 Is Transported to and Degraded in the Vacuole via Autophagy

In systematic analysis of the PAS localization of Atg proteins (12), Atg1 was found to be transported to the vacuole via autophagy. When yeast cells expressing Atg1 tagged with GFP were treated with the autophagy inducer rapamycin, GFP fluorescence in the vacuole increased in a wild-type strain, but not in atg mutants such as atg14Δ (Fig. 1A). Immunoblotting analysis using antibodies against GFP showed that wild-type cells produce GFP-containing fragments under autophagy-inducing conditions, but atg14Δ cells do not (Fig. 1B). These fragments were likely to have been produced by proteolysis of Atg1-GFP in the vacuole because their accumulation was blocked by the addition of phenylmethylsulfonyl fluoride (PMSF), which inactivates the vacuolar protease Prb1 (supplemental Fig. S1A). In contrast, specific fragments were not detected by immunoblotting using antibodies against Atg1 (supplemental Fig. S1B), suggesting that the Atg1 portion of the fusion protein is degraded in the vacuole, whereas the GFP portion is resistant to vacuolar proteases.

FIGURE 1.
Atg1 interacts with Atg8 via the AIM. A, wild-type (YNH512) and atg14Δ (YNH731) cells expressing Atg1-GFP were grown to mid-log phase, treated with rapamycin for 6 h, and observed under a fluorescence microscope. Arrowheads indicate vacuoles. ...

Atg1 Directly Binds to Atg8 via the AIM

We next addressed the mechanism by which Atg1 is efficiently incorporated into the autophagosome. First, we examined the involvement of factors known to function in selective types of autophagy in yeast (1618). Deletion of genes encoding adaptor (Atg11) and receptor proteins (Atg19, Atg34, and Atg32) did not significantly affect the vacuolar transport of Atg1 (supplemental Fig. S2). However, a double mutation in Atg8 (P52A/R67A), which decreases its binding to the AIM but not its function in autophagosome formation (8, 19), retarded Atg1 transport to the vacuole (Fig. 1C). Although an as yet unidentified receptor containing the AIM might mediate Atg1 incorporation into the autophagosome, previous studies showed that not only receptors but also some core Atgs use the AIM to interact with Atg8 (20). Therefore, we examined the possibility that Atg1 directly interacts with Atg8 via the AIM. Atg1 consists of the N-terminal kinase domain, the C-terminal region responsible for binding to Atg13, and the intervening middle region (Fig. 1D). The yeast two-hybrid assay clearly showed that Atg1 binds to Atg8; an indicator strain coexpressing Atg1 fused to the Gal4 transcription activation domain and Atg8 fused to the Gal4 DNA-binding domain could grow on agar medium lacking histidine (Fig. 1E and supplemental Fig. S3). Furthermore, the Atg1 middle region alone interacted with Atg8, whereas Atg1 lacking this region did not, suggesting that Atg1 interacts with Atg8 via its middle region (Fig. 1E and supplemental Fig. S3). When compared with the full-length protein, the Atg1 middle region exhibited a higher affinity to Atg8; yeast strains coexpressing this Atg1 variant could also grow in the absence of adenine (Fig. 1E). We found that the P52A/R67A double mutation in Atg8 negatively affected the interaction of Atg8 with the Atg1 middle region (Fig. 1E), suggesting that the AIM binding ability of Atg8 is important for the interaction with Atg1.

Based on common features of known AIMs (20), we identified a putative AIM with the amino acid sequence Tyr429-Val430-Val431-Val432 in the middle region of Atg1 (Fig. 1D). When Tyr429 and Val432 were simultaneously replaced with alanine residues, the interaction between Atg1 and Atg8 was severely impaired (Fig. 1E). This was also confirmed by coimmunoprecipitation experiments (Fig. 1F). Although immunoprecipitation of Atg8 tagged with the 3×FLAG sequence coprecipitated wild-type Atg1-GFP, the efficiency was significantly reduced by the Y429A/V432A double mutation. Thus, we conclude that this sequence in Atg1 functions as the AIM.

Association of Atg1 with the Isolation Membrane Facilitates Autophagosome Formation

We showed that the mutations in the AIM abolished the vacuolar transport of Atg1 (Fig. 2, A and B). Given that Atg8 localizes to the isolation membrane and is retained on the complete autophagosomal membrane, these results suggest that Atg1 interacts with Atg8 using the AIM, thereby associates with the isolation membrane, and is eventually encapsulated into the autophagosome.

FIGURE 2.
Characterization of an AIM mutant of Atg1. A and B, atg1Δ cells (YNH204) expressing Atg1WT-GFP or Atg1AIM mut-GFP from single-copy plasmids were treated with rapamycin and analyzed by fluorescence microscopy (A) or immunoblotting with anti-GFP ...

To examine the autophagic activity of cells expressing the AIM mutant of Atg1, we performed an ALP assay (Fig. 2C and supplemental Fig. S4A) (15). The results revealed that the Atg1 AIM mutant cells exhibited a partial but significant defect in autophagy. In accordance with this, the vacuolar transport of GFP-Atg8 also decreased in the mutant cells (supplemental Fig. S4, B and C). We also examined accumulation of autophagic bodies, inner membrane vesicles released into the vacuolar lumen upon fusion between autophagosomal outer membranes and the vacuolar membrane, using vacuolar protease-deficient strains (supplemental Movie S1). The Atg1 AIM mutant cells accumulated autophagic bodies less than wild-type cells. These results suggest that the mutations in the AIM of Atg1 decrease the efficiency of autophagosome formation. Coimmunoprecipitation analysis showed that the Atg1 AIM mutant interacted normally with Atg13 and Atg17, suggesting that the mutations in the AIM did not affect the formation of the PAS-scaffolding complex (Fig. 2D). In wild-type cells, Atg1 kinase activity is up-regulated under autophagy-inducing conditions (10), which can be assessed by the appearance of a slower migrating band of autophosphorylated Atg1 in immunoblotting analysis (21). That band was detected in the AIM mutant at a level similar to wild-type Atg1 and disappeared upon deletion of ATG13, which is essential for activation of Atg1 (Fig. 2E). Thus, the stimulation of Atg1 kinase activity occurred normally in the AIM mutant. Furthermore, the Atg1 AIM mutant localized to the PAS as efficiently as the wild-type protein (Fig. 2F and supplemental Fig. S5). It was also shown that the mutations in the AIM of Atg1 did not reduce the PAS localization of Atg8; it rather accumulated at the PAS, probably due to delayed autophagosome formation (Fig. 2G and supplemental Fig. S5). Taken together, these results suggest that mutations in the AIM of Atg1 cause a defect in autophagy by abolishing Atg1 association with the isolation membrane rather than by affecting the functions of Atg1 involved in the initiation of autophagosome formation.

A double mutation in the C-terminal region of Atg1, Y878A/R885A, impairs the interaction with Atg13, resulting in reduced PAS localization of Atg1 (13). This Atg1 mutant also exhibited a defect in PAS localization when it was coexpressed with wild-type Atg1 (supplemental Fig. S6). We showed that this mutant was less efficiently transported to the vacuole than wild-type Atg1 (Fig. 2, H and I), although autophagy occurred normally in these cells. These results suggest that PAS localization is a prerequisite for Atg1 to associate with autophagosomal membranes.

DISCUSSION

Previous studies have established the role of Atg1 kinase and its regulatory proteins in the initiation of autophagosome formation; in response to autophagy-inducing signals, Atg1 forms a large complex with Atg13 and the Atg17-Atg29-Atg31 ternary complex. This complex further forms a higher-order assembly that serves as a scaffold, which organizes the PAS and induces autophagosome formation. However, the autophagic transport of Atg1 to the vacuole suggested that the kinase remains associated with the autophagosomal membrane until a late stage of its formation. In contrast, few of the regulatory proteins are transported to the vacuole. These data indicated that Atg1 plays an additional role, distinct from its function in triggering autophagosome formation in association with the regulators. In agreement with these observations, it has recently been shown that Atg1, but not its regulators, localizes to the isolation membrane.3 In the present study, our results suggest that the association of Atg1 with the isolation membrane is mediated by direct interaction with Atg8. Mutations that disrupt this interaction, and thereby prevent Atg1 association with the membrane, caused a significant defect in autophagy, although they did not impair Atg1 functions involved in the initiation of autophagosome formation. Thus, in accordance with the above notion, our results suggest that Atg1 plays an additional role in promoting autophagosome formation on the isolation membrane.

The interaction between Atg1 and Atg8 involves an AIM located in the middle region of Atg1. The AIM was first identified as a sequence commonly seen in receptors for selective autophagy and subsequently found in core Atg proteins such as yeast Atg3 and mammalian Atg4 (20). Although the AIM in Atg3 is specifically required for the cytoplasm-to-vacuole targeting pathway, which can be regarded as a type of selective autophagy, this study identified for the first time the AIM involved in starvation-induced, nonselective autophagy.

Most core Atg proteins localize to the PAS in a hierarchical manner; the PAS-scaffolding complex assembles first, whereas Atg8 is one of the most downstream factors (1113). We showed that AIM mutations do not significantly affect the PAS localization of either Atg1 or Atg8. Therefore, it seems that the AIM-mediated interaction between Atg1 and Atg8 is not involved in PAS organization based on the hierarchical model. We also showed that an Atg1 mutant defective in interaction with Atg13, and thus in PAS localization, is not efficiently transported to the vacuole. These results suggest that Atg1 in complex with the regulators first assembles to organize the PAS, induces the formation of the isolation membrane by recruiting downstream factors including Atg8, and is subsequently dissociated from the complex and transferred to Atg8-PE on the membrane. We propose that the role of Atg1 on the isolation membrane is to facilitate membrane expansion by phosphorylating some protein(s) on the membrane. These target(s) of Atg1 kinase may not be identical to those phosphorylated by Atg1 in the early stage of autophagosome formation; identification of these targets will require further study.

Interactions between Atg1 and Atg8 homologs have also been reported in mammals and plants (22, 23). Although the details of these interactions remain to be elucidated, the AIM in Atg1 homologs may mediate their interactions with Atg8 homologs in higher eukaryotes as well. Indeed, amino acid sequences predicted to function as an AIM are found in Arabidopsis Atg1 homologs (supplemental Fig. S7). However, the significance of the AIM-mediated interaction between Atg1 and Atg8 may differ among organisms. In plant cells, unlike yeast, Atg13 is also transported to the vacuole via autophagy (23). In contrast, the autophagic transport of ULK1 to the lysosome has not been observed in mammalian cells. These differences may be relevant to the different organization of the Atg1 complex and its behavior in response to autophagy-inducing signals in these organisms (24). Therefore, it will be important to elucidate how the interaction of Atg1 with Atg8 contributes to autophagy in mammals and plants. Our study provides a framework to address these issues.

Supplementary Material

Supplemental Data:

Acknowledgments

We thank the members of our laboratories for materials, helpful discussions, and technical support, and Yoko Hara for her secretarial support.

*This work was supported in part by the Funding Program for Next Generation World-Leading Researchers (to H. N.) and grants-in-aid for scientific research (to Y. O.) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

An external file that holds a picture, illustration, etc.
Object name is sbox.jpgThis article contains supplemental Methods, Tables S1 and S2, Figs. S1–S7, and Movie S1.

3K. Suzuki, M. Akioka, C. Kondo, and Y. Ohsumi, unpublished results.

2The abbreviations used are:

Atg
autophagy-related
AIM
Atg8 family interacting motif
PE
phosphatidylethanolamine
PAS
pre-autophagosomal structure
ALP
alkaline phosphatase
OD
optical density
mut
mutant
SC
synthetic complete.

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