PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of narLink to Publisher's site
 
Nucleic Acids Res. 1976 December; 3(12): 3255–3269.
PMCID: PMC343172

Kinetic studies on the cleavage of adenovirus DNA by restriction endonuclease Eco RI.

Abstract

The kinetics of cleavage of DNA from Adenovirus Type 1 (Ad1), Type 5 (Ad5) and Type 6 (Ad6) by restriction endonuclease EcoRI was investigated by quantitative evaluation of the fluorescence from ethidium stained DNA fragments separated on agarose gels. The apparent rate constants of cleavage at different cleavage sites have been determined and large differences in the cleavage rates of the individual sites within one type of DNA were found. From the kinetics of cleavage information on the sequence of the DNA fragments can be obtained. The order of the fragment A, B, C, D of Ad6 DNA obtained after complete cleavage by restriction endonuclease Eco RI was found to be A-D-C-B; the order of the corresponding fragments A, B, C of Ad1 and Ad5 DNA was found to be A-C-B.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Pettersson U, Mulder C, Deluis H, Sharp PA. Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A. 1973 Jan;70(1):200–204. [PubMed]
  • Mulder C, Arrand JR, Delius H, Keller W, Pettersson U, Roberts RJ, Sharp PA. Cleavage maps of DNA from adenovirus types 2 and 5 by restriction endonucleases EcoRI and HpaI. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):397–400. [PubMed]
  • Polisky B, Greene P, Garfin DE, McCarthy BJ, Goodman HM, Boyer HW. Specificity of substrate recognition by the EcoRI restriction endonuclease. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3310–3314. [PubMed]
  • Pettersson U, Tibbetts C, Philipson L. Hybridization maps of early and late messenger RNA sequences on the adenovirus type 2 genome. J Mol Biol. 1976 Mar 15;101(4):479–501. [PubMed]
  • Tibbetts C, Pettersson U. Complementary strand-specific sequences from unique fragments of adenovirus type 2 DNA for hybridization-mapping experiments. J Mol Biol. 1974 Oct 5;88(4):767–784. [PubMed]
  • Tibbetts C, Pettersson U, Johansson K, Philpson L. Relationship of mRNA from productively infected cells to the complementary strands of adenovirus type 2 DNA. J Virol. 1974 Feb;13(2):370–377. [PMC free article] [PubMed]
  • Sambrook J, Sharp PA, Keller W. Transcription of Simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells. J Mol Biol. 1972 Sep 14;70(1):57–71. [PubMed]
  • Garon CF, Berry KW, Hierholzer JC, Rose JA. Mapping of base sequence heterologies between genomes from different adenovirus serotypes. Virology. 1973 Aug;54(2):414–426. [PubMed]
  • Sambrook J, Williams J, Sharp PA, Grodzicker T. Physical mapping of temperature-sensitive mutations of adenoviruses. J Mol Biol. 1975 Sep 25;97(3):369–390. [PubMed]
  • Thomas M, Davis RW. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975 Jan 25;91(3):315–328. [PubMed]
  • Greene PH, Poonian MS, Nussbaum AL, Tobias L, Garfin DE, Boyer HW, Goodman HM. Restriction and modification of a self-complementary octanucleotide containing the EcoRI substrate. J Mol Biol. 1975 Dec 5;99(2):237–261. [PubMed]
  • Lin S, Riggs AD. The general affinity of lac repressor for E. coli DNA: implications for gene regulation in procaryotes and eucaryotes. Cell. 1975 Feb;4(2):107–111. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press