First isolation and characterization of Chryseobacterium shigense from rainbow trout

doi:10.1186/1746-6148-8-77

BMC Vet Res. 2012; 8: 77.

Published online 2012 June 7. doi: 10.1186/1746-6148-8-77

PMCID: PMC3426477

First isolation and characterization of Chryseobacterium shigense from rainbow trout

Leydis Zamora,¹ Ana I Vela,^1,² Mª Angel Palacios,³ Lucas Domínguez,¹ and José Francisco Fernández-Garayzábal^1,²

¹Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense, 28040 Madrid, Spain

²Departamento de Sanidad Animal. Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain

³Grupo Piszolla, S.L, 37800, Alba de Tormes, Salamanca, Spain

Corresponding author.

Leydis Zamora: lzamoram/at/visavet.ucm.es; Ana I Vela: avela/at/vet.ucm.es; Mª Angel Palacios: m.angel/at/piszolla.com; Lucas Domínguez: lucasdo/at/visavet.ucm.es; José Francisco Fernández-Garayzábal: garayzab/at/vet.ucm.es

Received July 12, 2011; Accepted June 7, 2012.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

There have been an increasing number of infections in fish associated with different species of Chryseobacterium, being considered potentially emerging pathogens. Nevertheless the knowledge of the diversity of species associated with fish disease is partial due to the problems for a correct identification at the species level based exclusively on phenotypic laboratory methods.

Results

Chryseobacterium shigense was isolated from the liver, kidney and gills of diseased rainbow trout in different disease episodes that occurred in a fish farm between May 2008 and June 2009. Identity of the isolates was confirmed by 16

S rRNA gene sequencing and phenotypic characterization. Isolates represented a single strain as determined by random amplified polymorphic DNA analysis.

Conclusions

This is the first description of the recovery of C. shigense from clinical specimens in trout, a very different habitat to fresh lactic acid beverage where it was initially isolated.

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