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Biotechnol Res Int. 2012; 2012: 904763.
Published online Jul 15, 2012. doi:  10.1155/2012/904763
PMCID: PMC3418636
Experimental Investigations on the Effects of Carbon and Nitrogen Sources on Concomitant Amylase and Polygalacturonase Production by Trichoderma viride BITRS-1001 in Submerged Fermentation
Arotupin Daniel Juwon 1 and Ogunmolu Funso Emmanuel 2 *
1Department of Microbiology, Federal University of Technology Akure, PMB 704, Akure, Ondo State, Nigeria
2Department of Microbiology and Biotechnology, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria
*Ogunmolu Funso Emmanuel: blessedfunso/at/yahoo.co.uk
Academic Editor: Yu Hong Wei
Received March 31, 2012; Revised May 23, 2012; Accepted June 6, 2012.
Abstract
The paper investigates the effects of different commercial carbon and nitrogen sources on the concomitant synthesis of amylase and polygalacturonase enzymes with the aim of optimizing them for maximal enzyme production. The microorganism used in this work was the fungus Trichoderma viride BITRS-1001, which had been previously identified as a highly active producer of amylase and polygalacturonase enzymes. The results showed that the different commercial carbon and nitrogen substrate significantly affected the concomitant syntheses of amylase and polygalacturonase in culture media supplemented with the different commercial carbon and nitrogen substrates. The result obtained suggested that for optimal and concomitant synthesis of the enzymes by Trichoderma viride BITRS-1001 in submerged fermentation, minimal medium supplemented with maltose and casein were the carbon and nitrogen substrates of choice.
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