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Logo of bmcvetresBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Veterinary Research
 
BMC Vet Res. 2012; 8: 61.
Published online May 20, 2012. doi:  10.1186/1746-6148-8-61
PMCID: PMC3416721
Clinical, haematological and biochemical responses of sheep undergoing autologous blood transfusion
Rejane Santos Sousa,1 Dowglish Ferreira Chaves,1 Raimundo Alves Barrêto-Júnior,1 Isadora Karolina Freitas Sousa,2 Herbert Sousa Soares,1 Isabella Oliveira Barros,1 Antonio Humberto Hamad Minervino,2 and Enrico Lippi Ortolanicorresponding author2
1Federal Rural University of Semi-Arid, Av. Francisco Mota, 572, Bairro Costa e Silva, CEP: 59.625-900, Mossoró, RN, Brazil
2Department of Clinical Sciences, College of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87 - Cidade Universitária, CEP 05508 270, São Paulo, SP, Brazil
corresponding authorCorresponding author.
Rejane Santos Sousa: rejane.santossousa/at/gmail.com; Dowglish Ferreira Chaves: dowglish/at/hotmail.com; Raimundo Alves Barrêto-Júnior: barreto/at/ufersa.edu.br; Isadora Karolina Freitas Sousa: isadoramedvet/at/hotmail.com; Herbert Sousa Soares: hssfortal/at/yahoo.com.br; Isabella Oliveira Barros: doutorabella/at/hotmail.com; Antonio Humberto Hamad Minervino: hhamad/at/usp.br; Enrico Lippi Ortolani: enrico.ortolani/at/pq.cnpq.br
Received November 3, 2011; Accepted May 20, 2012.
Abstract
Background
This study aimed to evaluate the clinical, haematological and biochemical responses to autologous blood transfusion and the feasibility of this practice in sheep. Thus, we used eight male, 8 months old sheep, weighing on average 30 kg, from which 15 mL/kg of whole blood was collected and stored in CPDA-1 bags. Blood samples were refrigerated for 8 days and subsequently re-infused. The clinical, haematological and biochemical parameters were evaluated before blood collection and reinfusion, after 10 minutes of collection and reinfusion, after 3, 6, 12, 24, 48, 96 and 192 hours after collection and reinfusion.
Results
With respect to clinical parameters, we observed a decrease in heart rate after 24, 48 and 196 hours from reinfusion compared to basal values (p < 0.05). Haematological variables including globular volume and erythrocyte counts showed a significant decrease (p < 0.01) at all time points after collection and increased (p < 0.01) at all time points after reinfusion. There was a significant increase in total protein and calcium at all time points after reinfusion (p < 0.05).
Conclusion
Autologous transfusion in sheep slightly altered the physiological, biochemical and haematological responses of sheep, indicating that the technique proposed is safe and can be applied in the clinical practice of this species. The 8 d period was not sufficient for complete recovery of the haematological parameters after blood collection.
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