PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of bmcbiotBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Biotechnology
 
BMC Biotechnol. 2012; 12: 22.
Published online May 6, 2012. doi:  10.1186/1472-6750-12-22
PMCID: PMC3416697
Untranslated regions of diverse plant viral RNAs vary greatly in translation enhancement efficiency
Qiuling Fan,1,2 Krzysztof Treder,1 and W Allen Millercorresponding author1
1Department of Plant Pathology & Microbiology, and Center for Plant Responses to Environmental Stresses, Iowa State University, Ames, IA, 50011, USA
21615 E 8th St, #6, Davis, CA, 95616, USA
corresponding authorCorresponding author.
Qiuling Fan: qlfanhn/at/gmail.com; Krzysztof Treder: ktreder/at/iastate.edu; W Allen Miller: wamiller/at/iastate.edu
Received December 23, 2011; Accepted May 6, 2012.
Abstract
Background
Whole plants or plant cell cultures can serve as low cost bioreactors to produce massive amounts of a specific protein for pharmacological or industrial use. To maximize protein expression, translation of mRNA must be optimized. Many plant viral RNAs harbor extremely efficient translation enhancers. However, few of these different translation elements have been compared side-by-side. Thus, it is unclear which are the most efficient translation enhancers. Here, we compare the effects of untranslated regions (UTRs) containing translation elements from six plant viruses on translation in wheat germ extract and in monocotyledenous and dicotyledenous plant cells.
Results
The highest expressing uncapped mRNAs contained viral UTRs harboring Barley yellow dwarf virus (BYDV)-like cap-independent translation elements (BTEs). The BYDV BTE conferred the most efficient translation of a luciferase reporter in wheat germ extract and oat protoplasts, while uncapped mRNA containing the BTE from Tobacco necrosis virus-D translated most efficiently in tobacco cells. Capped mRNA containing the Tobacco mosaic virus omega sequence was the most efficient mRNA in tobacco cells. UTRs from Satellite tobacco necrosis virus, Tomato bushy stunt virus, and Crucifer-infecting tobamovirus (crTMV) did not stimulate translation efficiently. mRNA with the crTMV 5′ UTR was unstable in tobacco protoplasts.
Conclusions
BTEs confer the highest levels of translation of uncapped mRNAs in vitro and in vivo, while the capped omega sequence is most efficient in tobacco cells. These results provide a basis for understanding mechanisms of translation enhancement, and for maximizing protein synthesis in cell-free systems, transgenic plants, or in viral expression vectors.
Articles from BMC Biotechnology are provided here courtesy of
BioMed Central