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Logo of bmcmudisBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Musculoskeletal Disorders
 
BMC Musculoskelet Disord. 2012; 13: 130.
Published online Jul 24, 2012. doi:  10.1186/1471-2474-13-130
PMCID: PMC3416648
Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy kashin-beck disease
Feng Zhang,1 Xiong Guo,corresponding author1 Weizhuo Wang,2 Shixun Wu,1 Weijuan Ma,1 and Hua Yan3
1 Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Faculty of Public Health, College of Medicine, Xi’an Jiaotong University, Xi’an, Shaanxi, People’s Republic of China, 710061
2Department of Orthopedics Surgery, The Second Affiliated Hospital, College of Medicine, Xi’an Jiaotong University, Xi’an, Shaanxi, 710004, People’s Republic of China
3National Engineering Research Center for Miniaturized Detection Systems, Northwest University, Xi’an, Shaanxi, 710069, People’s Republic of China
corresponding authorCorresponding author.
Feng Zhang: fzhxjtu/at/mail.xjtu.edu.cn; Xiong Guo: guox/at/mail.xjtu.edu.cn; Weizhuo Wang: wangweizhuo/at/mail.xjtu.edu.cn; Shixun Wu: wsx19821115/at/163.com; Weijuan Ma: janemwj/at/yahoo.com.cn; Hua Yan: lental1798/at/sina.com
Received March 1, 2012; Accepted July 24, 2012.
Abstract
Background
Kashin-Beck Disease (KBD) is an endemic osteochondropathy. Mycotoxins are believed to play an important role in the pathogenesis of KBD. Because the molecular mechanism of mycotoxin-induced cartilage lesions remains unclear, there is not effective treatment for KBD now. To identify key genes involved in the mycotoxin-induced cartilage lesions, we compared the expression profiles of mycotoxin-related genes (MRG) between KBD cartilage and healthy cartilage.
Methods
Total RNA was isolated from cartilage samples, following by being amplified, labeled and hybridized to Agilent human whole genome microarray chip. qRT-PCR was conducted to validate the microarray data. 1,167 MRG were derived from the environmentally related genomic database Toxicogenomics. The microarray data of MRG was subjected to single gene and gene ontology (GO) expression analysis for identifying differently expressed genes and GO.
Results
We identified 7 up-regulated MRG and 2 down-regulated MRG in KBD cartilage, involved in collagen, apoptosis, metabolism and growth & development. GO expression analysis found that 4 apoptosis-related GO and 5 growth & development-related GO were significantly up-regulated in KBD cartilage.
Conclusions
Based on the results of previous and our studies, we suggest that mycotoxins might contribute to the development of KBD through dysfunction of MRG involved in collagen, apoptosis and growth & development in cartilage.
Keywords: Kashin-beck disease, Mycotoxins, Microarray
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