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Introduction. Preoperative identification of patients with inadequate hamstring grafts for anterior cruciate ligament reconstruction is still a subject of interest. Purpose. The purpose of this study was to determine whether the semitendinosus tendon length is adequate for four-strand graft harvested by common technique (without bone plug) and whether there is correlation of gracilis and semitendinosus tendon grafts length and diameter of quadrupled graft with anthropometric parameters. Materials and Methods. In this retrospective study, 61 patients (45 males, 16 females) undergoing ACL reconstruction using four-strand hamstring autograft tendons were included. Results. The length of semitendinosus tendon, harvested by the common technique, was in 21% of our cases inadequate in order to be used alone as a four-strand graft especially in females (43%). There was moderate correlation between semitendinosus and gracilis graft diameter and patient's height and weight and fair correlation to BMI. We found no statistically important predictor for graft diameter in female patients. Conclusions. The length of semitendinosus tendon, harvested by common technique, is usually inadequate to be used alone as a four-strand graft especially in females. The most reliable predictor seems to be patient's height in males. In female patients, there is no statistically important predictor.
The anterior cruciate ligament (ACL) is the most commonly reconstructed ligament of the knee . An injury to the ACL can result in significant functional impairment . Strength and stiffness of the graft are important components in order to decide the kind of graft and the technique of tendon replacement.
It is widely accepted that four-strand hamstring autograft represents a successful option for ACL reconstruction [3–7]. A possible complication when using both the semitendinosus (ST) and gracilis (G) tendon graft is that of hamstring strength deficit in deep flexion and internal rotation [8–10]. Gobbi and Francisco suggest to use only ST tendon in a four-strand graft with bone plug in order to reduce donor's site morbidity and to increase graft's diameter , while later on in another study Gobbi again suggests a double bundle using only semitendinosus tendon for better functional rehabilitation of the knee . In this type of operations, it would be essential for the surgeon to be able to predict preoperatively graft length in order to choose the ideal graft type and to avoid scar formation, pain, operating time and infection risk. Prediction of graft length could also be useful in cases of revision ACL reconstruction where usually a larger diameter graft is needed  or in cases of active and/or highly demanding patients or professional athletes where larger diameter grafts would be ideal.
Scott and Insall report that the length of normal ACL is 38mm (25–41mm) and the width is 10mm (7–12mm), on average . In order to assure the optimal 7cm quadrupled graft construct for ACL reconstruction (2cm in the femoral tunnel, 3cm intra articular, and 2cm in the tibial tunnel), it is essential to obtain a minimum tendon length of 28cm (ranged from 28 to 30cm) with a minimum thickness of 7mm [11, 15–17].
The purpose of this retrospective study was to determine whether alone the ST tendon length is adequate for four-strand graft harvested by common technique and whether there is correlation of G and ST grafts length and diameter with anthropometric parameters.
Sixty-one consecutive patients (45 males—16 females) undergoing ACL reconstruction using four-strand hamstring autograft tendons were included in this retrospective study. Age, gender, height, weight, and Body Mass Index (BMI) for each patient were recorded preoperatively.
Surgical operation was performed by the same surgeon in all cases, and hamstring tendon autografts (ST-G) were harvested by the same way. An oblique incision was performed on the skin over the pes anserinus attachment area on the proximal tibia. Subcutaneous fat was incised and attentive blood hemostasis was performed. The sartorius fascia was incised parallel to the direction of G tendon. On the next step, G tendon was dissected (Figure 1). The tendon was removed from its proximal attachment with a close tendon stripper (Figure 2). The detachment of the tendon on its tibial end was done close to the bone in order to preserve its maximum length (Figure 3). The same procedure was followed for ST tendon but before removal of its proximal attachment, the tendon band towards gastrocnemius muscle was dissected with a scissor under direct vision. After both hamstring tendons harvest each one of them became double strand to create a four-strand graft with both tendons. Each end of the tendon grafts was stitched with a no. 2 nonabsorbable polyester suture. After blunt removal of attached muscle and fat but before any further postharvested alteration of trimming of the graft, intraoperative measurements of both tendons were done, such as length of each and diameter of the quadrupled graft using sizing cylinders with incremental size change of 0.5mm. The graft diameter was considered to be the dimension of the smallest cylinder that could pass through (Figure 4). In case that the graft diameter was not exactly fitted in a specific 0.5mm increment, the size of the graft was recorded according to the drill size of our tunnels. Pretensioning of the graft on the surgical table was not performed. Finally single-bundle ACL reconstruction was performed.
Adequate length of ST tendon graft as only four-strand graft for single-bundle ACL reconstruction was considered to be 28cm. Adequate diameter of four-strand hamstring autograft (ST-G) was considered to be more than 7mm.
In statistical analysis, independent samples t-test was used to identify differences between the mean values of continuous variables according to gender. Chi-square statistics (χ2) was done to investigate any possible association of the categorical variables with the diameter of the graft. Bivariate correlation coefficients (Pearson r) and multiple linear regressions were calculated to evaluate any possible association between clinical data and intraoperatively measured hamstring graft lengths and diameters. Higher correlation coefficients indicate stronger relationships between variables. Statistical package SPSS version 18.0; SPSS, Chicago, III for Windows was used for analyses. A P value of 0.01 was taken as the level of significance.
Anthropometric measurements including the average age, weight, height and BMI, and gender of patients participating in this study are shown in Table 1. Graft characteristics are described according to gender in Table 2. Frequency of adequate ST tendon graft length according to gender is presented in Table 3. Frequency of adequate four-strand hamstring (ST-G) autograft diameter according to gender is summarized in Table 4.
Female patients were lighter and shorter with lower BMIs, and had shorter length and smaller diameter hamstring grafts with statistical significance in comparison with males.
Linear regression analyses separated by gender, considering height and BMI had showed existing statistically important correlation with graft size (diameter) only in males (Figures (Figures55 and and6).6).
Pearson's correlation tests analysis in the whole sample indicates that hamstring graft size (diameter) was correlated to patient's height, BMI, and also weight. Height and weight of patients was correlated with length of G and ST tendon graft but not BMI (Table 5). According to gender, Pearson's correlation tests analysis considering weight, height, and BMI had shown existing statistically important correlation with graft size (diameter) only in males (Table 6).
Simple linear regression indicated that patient BMI and height explained approximately 17.1% and 24.7%, respectively, of the variation in quadrupled graft diameter.
Through regression analysis, we constructed the following predictive equations for quadrupled graft diameter:
These equations indicate that patients with BMI less than 19.875 and less than 170cm tall whose weight is less than 57.4kg are at highest risk for having a hamstring graft less than 7mm in diameter.
Also simple linear regression for graft lengths indicated that height explained approximately 13.9% of variance in G tendon length and 19.4% of the variance in ST length. Through regression analysis, we came up with the following predictive equations for G graft length (GL) and ST graft length (SL):
These equations indicate that patients with height less than 167cm are at highest risk for having an inadequate semitendinosus graft tendon less than 28cm in length.
When we separated these analyses by gender, we found that height and probably BMI only referring to G length were the best predictors for graft diameter in male patients. We found no statistically important predictor for graft diameter in female patients (Table 6).
In our study in one out of five patients (21%) the length of ST tendon, harvested by the common technique, was inadequate in order to be used alone as a four-strand graft. Especially in female patients, the length of ST tendon was less than 28cm in 43.75%. Moreover, according to our findings, height and weight are considered to be moderate predictors of the adequacy of the semitendinosus tendon length when using alone ST four-strand graft or of the four-strand ST and G graft diameter for ACL single-bundle reconstruction harvested by common technique (without bone plug). The most reliable predictor seems to be patient's height in males. In female patients, there is no such statistically important predictor.
The use of ST and G grafts seems to have good results in many studies [18–21], while other studies report similar results by the use of ST only tendon as a quadrupled graft in reconstruction of ACL [22–24]. Gobbi et al. recommended using only one tendon whenever possible because the ST alone seem to have an advantage over the ST-G construct with regard to internal rotation weakness following harvest of two tendons, although there is not much clinical difference in both techniques . In order to assure the optimal 7cm quadrupled graft construct (2cm in the femoral tunnel, 3cm intra articular, and 2cm in the tibial tunnel), it is essential to obtain a minimum tendon length of 28cm (ranged from 28 to 30cm) . Increased research of double-bundle reconstruction and development of new operative techniques necessitate preoperative planning of size parameters for ideal graft choice . Furthermore, a new technique of ACL reconstruction with double-bundle, single tendon (ST) seems to offer the possibility of reconstructing both the AM and PL bundles without disrupting the function of hamstring muscles. This is achieved due to preservation of gracilis tendon, which offers stability in deep flexion and internal rotation strength and protects from further ACL injuries . But even in this case, the minimum graft length needed is 28cm (2cm in the femoral tunnel, 3cm intra-articular, and 2cm in the tibial tunnel) . Additionally, it has been demonstrated that the average diameter of the normal ACL is 11mm; therefore, a graft of minimum thickness of 7mm is recommended [15–17]. The thicker the graft is the stronger and stiffer the graft will be. The biomechanical properties of the graft are certainly affected by its diameter.
According to Vernon et al., the use of ST tendon alone is adequate in almost all cases  and the rate of insufficiency for a quadrupled reconstruction is only one in 300 cases and is almost always the result of improper graft harvest . In contrast to our results regarding the adequacy of semitendinosus tendon as a four-strand graft for ACL reconstruction, the ST graft length was inadequate (i.e., shorter than 28cm) in 21% of all our patients and in 18% it was marginally adequate (28cm) and only in 61% of our patients semitendinosus tendon graft length was longer than 28cm. This is a high percent of possible cases in which ST four-strand tendon graft could be inadequate for ACL reconstruction and additional G tendon graft would be needed and comes in contrast to claims of other authors who support and recommend to use of only one tendon whenever possible . Referring to female patients, these rates are more impressive, while in 43.75% of all cases the ST graft length was inadequate, and in 18.75% it was marginally adequate and only in 37.5% of our female patients ST tendon graft length was longer than 28cm (Table 3). Additionally simple linear regression for graft lengths indicated that patients with height less than 167cm are at highest risk for having an inadequate ST graft tendon less than 28cm in length.
Referring to graft diameter and according to our results, the majority of patients (86.7%) had an adequate quadrupled graft diameter (7 to 8mm), while 10% of patient's grafts were inadequate (less than 7mm). Referring to female patients, this percent becomes 25%. Only 2 patients (3.3%) had graft diameter greater than 8mm (Table 4). Pinheiro et al. report that males with height equal to or greater than 1.80m achieved a higher percentage of 9mm grafts and larger average of graft diameter in comparison to the other patients with a height less than 1.80m males or females or both . In our sample, the two men with graft diameter greater than 8mm had height greater than 1.80m (1.83m and 1.90m). The hypothesis of Pinheiro et al.  is confirmed in our cases, but we cannot reach to safe conclusion because of the small number of our cases.
Hamstring graft size according to our study could be predicted by evaluating preoperatively some simple anthropometric parameters. According to our results ST, and G graft diameter was most strongly correlated to patient's weight (moderate correlation, r = 0.567), then to height (moderate correlation, r = 0.498) and finally to BMI (fair correlation, r = 0.414). Treme et al. in a study of 50 consecutive patients observed a positive effect of the BMI on graft diameter  in contrast to Tuman et al. and Pinheiro et al. who claim that BMI does not influence graft diameter [28, 30]. Referring to patient's weight in the study of Pinheiro et al. had less influence in graft diameter, contrary to us and to Treme et al. who found the strongest correlation with weight [28, 29]. Finally Schwatzberg et al. found moderate correlation between weight and graft diameter in a study of 119 consecutive patients . In another series of 536 patients, height was found to be a strong predictor of quadrupled hamstring graft diameter in 234 male patients .
The lengths of the hamstring graft can also be predicted by preoperative anthropometric measurements. In our study, the length of G and ST graft was most strongly correlated with height (fair correlation, r = 0.441) and then with weight (weak correlation, r = 0.369) of patient's, but there was no correlation with BMI. Also in a study of 80 patients, Pinheiro et al. claim that height is the most important variable that influences most the graft length . Treme et al. noted that height and leg length were strongly correlated with the hamstring tendon lengths . Chiang et al. in a study of 100 patients conclude that the patients' height could be used to predict both ST and G tendon lengths in Chinese patients . Tuman et al. after studying 106 patients concluded that height was also the most important variable but mainly in women . Schwatzberg et al. claim weak correlation to patient's height .
We found no statistically important predictor for graft diameter in female patients. Female patients were significantly lighter and shorter with lower BMIs and had shorter length and smaller diameter hamstring grafts in comparison with males. This result is in accordance with studies of Tuman et al., and Treme et al., who claimed that, mean values of graft diameter as well as weight and height in males were greater than in females [29, 30]. Chiang et al. also in their findings showed that men had significantly longer tendons than women . In our study, simple linear regression for graft diameter indicated that patients with BMI less than 19.875 and less than 170cm tall whose weight is less than 57.4kg are at highest risk for having a hamstring graft less than 7mm in diameter.
There are some limitations in our study. Firstly, the sample of female patients is not adequate in order to exact secure conclusions. Moreover, we were unable to investigate if smaller size of hamstring graft tendon in women were related to gender or to the smaller average anthropometric measurements . This was due to the fact that our groups according to gender had great differences of anthropometric variances. Secondly, we recognize the fact that our results could be influenced by the size of the sample, which could influence our data and as a consequence our results. For example, the correlation of BMI and the length of the graft could change if we had operated patients with great BMI. However, we believe that this patient group is a representative sample of patients that we operate for ACL deficiency. Thirdly, we did not evaluate the different level of sport activity of our patients and any possible correlation with graft diameter of length. Finally, in some cases the graft diameter was not exactly fitted in a specific 0.5mm increment. In these cases, the size of the graft was recorded according to the drill size of our tunnels.
The clinical relevance of this study showed that in shorter or female patients, there was a relatively higher risk of obtaining inadequate individual hamstring tendon lengths for double-bundle anterior cruciate ligament reconstruction procedures. Moreover, in our surgical practice, we used to harvest first the G tendon and then the ST tendon. After this study, we have altered our technique. The clinical importance of these findings and our suggestion is that ST tendon graft removal should always be performed before G tendon harvesting, and according to its adequacy of length (>28cm), the surgeon should decide whether further augmentation of the ACL graft with G tendon would be necessary.
Hamstring grafts less than 7mm in diameter and 28cm in length are not so rare. According to our findings we come to the conclusion that the length of ST tendon, harvested by the common technique, is usually inadequate in order to be used alone as a four-strand graft especially in females. Identification of these patients is still a subject of research. The potential of size prediction of autograft hamstring tendons in ACL reconstruction could contribute to choose the best graft and surgical technique individualized on patient's needs and in accordance with their special characteristics. By that way the possibilities of a good postsurgical result would be multiplied in difficult cases of patients like women, children, and professional athletes or revision of ACL. Also special surgical techniques such as quadrupled ST double-bundle ACL reconstruction and the DBST (double bundle, single-tendon) technique could be used more wisely avoiding unnecessary complications such as scar formation, pain, and operating time and infection risk. Height and weight are considered to be moderate predictors of the adequacy of the semitendinosus tendon length when using alone ST four-strand graft or of the four-strand ST and G graft diameter for ACL single-bundle reconstruction harvested by common technique (without bone plug). The most reliable predictor seems to be patients' height in males. In female patients, there is no such statistically important predictor.