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Logo of annaldermaAnnals of DermatologyThis ArticleInformation for AuthorsOnline Submission
 
Ann Dermatol. 2012 August; 24(3): 261–266.
Published online 2012 July 25. doi:  10.5021/ad.2012.24.3.261
PMCID: PMC3412233

Epigenetic Modulation of Gene Expression during Keratinocyte Differentiation

Seung Ju Back, M.D.,* Myung Im, M.D.,* Kyung Cheol Sohn, Ph.D., Dae Kyoung Choi, Ph.D., Ge Shi, Ph.D., Nam Ji Jeong, M.D., Young Lee, M.D., Young Joon Seo, M.D., Chang Deok Kim, Ph.D., and Jeung Hoon Lee, M.D.corresponding author

Abstract

Background

Epigenetic modulation of gene expression occurs by various methods, including DNA methylation and histone modification. DNA methylation of specific genes may affect the chromatin structure, preventing access by the transcriptional machinery. Although gene expression is dramatically changed during keratinocyte differentiation, there is no evidence of epigenetic modulation during the process of epidermal stratification.

Objective

We investigated whether epigenetic modulation is involved in keratinocyte differentiation-specific gene regulation.

Methods

We used trypsin to produce epidermal fragmentation (named T1-T4) and performed a morphological analysis using hematoxylin-eosin stain and cytokeratin expression based on reverse transcription polymerase chain reaction. We then constructed a DNA methylation microarray.

Results

Each epidermal fragment showed morphological features of the epithelial layer. T1 represented the basal layer, T2 was the spinous layer, T3 was the granular layer, and T4 was the cornified layer. The level of the K14 proliferation marker was increased in the T1 fraction, and the level of K10 differentiation marker was increased in the T2-T4 fractions. Using a methylation microarray with the T1 and T4 fractions, we obtained many hypermethylated and hypomethylated genes from differentiated keratinocytes.

Conclusion

The importance of epigenetic modulation in target gene expression during keratinocyte differentiation is identified.

Keywords: Cell differentiation, DNA methylation, Epigenomics, Keratinocytes

Articles from Annals of Dermatology are provided here courtesy of Korean Dermatological Association and Korean Society for Investigative Dermatology