Search tips
Search criteria 


Logo of narLink to Publisher's site
Nucleic Acids Res. 1985 April 25; 13(8): 3005–3014.
PMCID: PMC341210

A mitochondrial reading frame which may code for a maturase-like protein in Saccharomyces cerevisiae.


In S. cerevisiae, the large oxi3/oli2 mitochondrial transcript contains the products of the oxi3, aap1 and oli2 genes and an unassigned reading frame, RF3. In the work presented here, we have completed the nucleotide sequence of RF3. We have shown that RF3 is composed of four fairly large ORFs which overlap within GC rich sequences. Furthermore, a shift of +1 base was found between each pair of consecutive reading frames. We discuss how these frameshifts could be removed to produce a 500 aminoacid long protein containing the two well conserved P1 and P2 oligopeptide sequences featuring several mitochondrial intron reading frames, suggesting, thereby, a RNA-maturase-like activity for the putative RF3 protein. In addition, we suggest that the insertion of GC clusters in a gene could provide a novel way of regulating its expression.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (894K), or click on a page image below to browse page by page.

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press