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Biosynthesis of nanoparticles has received increasing attention due to the growing need to develop safe, time-effective and environmentally friendly technologies for nano-materials synthesis. This paper reports the one pot green synthesis of silver nanoparticles (AgNPs) using the leaf bud extract of a mangrove plant, Rhizophora mucronata and their antimicrobial effects against aquatic pathogens. Highly stable AgNPs were synthesized by treating the mangrove leaf bud extract with aqueous silver nitrate solution at 15psi pressure and 121°C for 5 minutes.
The biosynthesized AgNPs were characterized by UV-visible spectrum, at 426nm. The X-Ray Diffraction (XRD) pattern revealed the face-centered cubic geometry of AgNPs. Fourier Transform Infra Red (FTIR) spectroscopic analysis was carried out to identify the possible biomolecules responsible for biosynthesis of AgNPs from the leaf bud extract. The size and shape of the well-dispersed AgNPs were documented with the help of High Resolution Transmission Electron Microscopy (HRTEM) with a diameter ranged from 4 to 26nm. However a maximum number of particles were observed at 4nm in size. The antibacterial effects of AgNPs were studied against aquatic pathogens Proteus spp., Pseudomonas fluorescens and Flavobacterium spp., isolated from infected marine ornamental fish, Dascyllus trimaculatus.
This study reveals that the biosynthesized AgNPs using the leaf bud extract of a mangrove plant (R. mucronata) were found equally potent to synthetic antibiotics. The size of the inhibition zone increases when the concentration of the AgNPs increased and varies according to species.