Table 1
buffy mutant larvae maintain smaller steady-state lipid and glycogen stores
| Wild-type | buffyH37 | buffyH37/wt | |
|---|---|---|---|
| Complete medium: | |||
| Nile Red fat body (mean luminosity) | 62.4 ± 3.6 (3) | 50.4 ± 2.5 (3) | 0.81 |
| Nile Red lysate (FU) | 323 ± 47 (4) | 260 ± 16 (4) | 0.80 |
| Triacylglyceride (μg TAG/μg protein) | 0.56 ± 0.05 (4) | 0.47 ± 0.04 (4) | 0.85 |
| Glycogen (ng glycogen/μg protein) | 1.54 ± 0.16 (6) | 1.30 ± 0.11 (5) | 0.85 |
| 20% medium: | |||
| Nile Red fat body (mean luminosity) | 62.2 ± 15.5 (3) | 46.3 ± 1.6 (3) | 0.74 |
| Number of lipid droplets/fat body cell | 30.7 ± 5.3 (3) | 20.7 ± 3.4 (3) | 0.67 |
| Perimeter of lipid droplets | 38.9 ± 3.4 (3) | 32.3 ± 2.2 (3) | 0.83 |
| Complete medium + added sucrose: | |||
| Triacylglyceride (μg TAG/μg protein) | 0.87 ± 0.07 (4) | 0.74 ± 0.10 (4) | 0.85 |
All experiments were performed on third instar larvae fed the indicated medium. All data are presented as averages ± SEM with number of biological replicates in parentheses. With the exception of mean luminosity of Nile Red fat body in complete medium (P = 0.05), all other differences are not significant (P > 0.1) as determined by Student's unpaired two-tailed t test. Nevertheless, the trend is similar across all measurements. For TAG assays, a representative of four biological replicates is presented. For technical reasons the mean luminosity cannot be compared across different media conditions (see Methods for experimental details).