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From:
Published online Jul 24, 2012. doi: 10.1186/1741-7007-10-63
Table 1
buffy mutant larvae maintain smaller steady-state lipid and glycogen stores
Wild-typebuffyH37buffyH37/wt
Complete medium:
Nile Red fat body (mean luminosity)62.4 ± 3.6 (3)50.4 ± 2.5 (3)0.81
Nile Red lysate (FU)323 ± 47 (4)260 ± 16 (4)0.80
Triacylglyceride (μg TAG/μg protein)0.56 ± 0.05 (4)0.47 ± 0.04 (4)0.85
Glycogen (ng glycogen/μg protein)1.54 ± 0.16 (6)1.30 ± 0.11 (5)0.85
20% medium:
Nile Red fat body (mean luminosity)62.2 ± 15.5 (3)46.3 ± 1.6 (3)0.74
Number of lipid droplets/fat body cell30.7 ± 5.3 (3)20.7 ± 3.4 (3)0.67
Perimeter of lipid droplets38.9 ± 3.4 (3)32.3 ± 2.2 (3)0.83
Complete medium + added sucrose:
Triacylglyceride (μg TAG/μg protein)0.87 ± 0.07 (4)0.74 ± 0.10 (4)0.85
All experiments were performed on third instar larvae fed the indicated medium. All data are presented as averages ± SEM with number of biological replicates in parentheses. With the exception of mean luminosity of Nile Red fat body in complete medium (P = 0.05), all other differences are not significant (P > 0.1) as determined by Student's unpaired two-tailed t test. Nevertheless, the trend is similar across all measurements. For TAG assays, a representative of four biological replicates is presented. For technical reasons the mean luminosity cannot be compared across different media conditions (see Methods for experimental details).
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