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Ectopic Buffy inhibits autophagy following 4 h of amino-acid starvation but does not block programmed autophagy. (A, B) LTR staining of fat bodies from 4 h-starved larvae expressing da-Gal4 (A) or da-Gal4; UAS-buffy (B). (C) TEM of fat body from da-Gal4; UAS-buffy after 4 h of starvation with no visible autophagic structures. (D-F) Autophagic vesicles are clearly visible in da-Gal4; UAS-buffy fat body from late third instar (wandering) larvae demonstrated by LTR staining (D), mCherry-Atg8a (E), and TEM (F); arrowheads point to autolysosomes). Scale bar = 47 μm (A, B, D, E) and 2.0 μm (C, F).