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Nucleic Acids Res. 1985 January 11; 13(1): 207–223.
PMCID: PMC340985

Structural features and restricted expression of a human alpha-tubulin gene.


The nucleotide sequence of a human alpha-tubulin gene (b alpha 1) is described. This gene is extensively homologous to a rat alpha-tubulin gene in its coding regions, 3'-untranslated region and, indeed, in segments of its largest intron. However, with the exception of three short conserved blocks of homology, the 5' flanking regions of the rat and human genes are unrelated. Hence, these genes each encoding an identical protein are transcribed under the influence of divergent promoters. Blot analyses using RNA from a variety of transformed cells derived from different tissues indicate that expression of the human alpha-tubulin gene is restricted to cells of neurological origin. Among neurological cell types b alpha 1 expression is further restricted to adherent cells that are morphologically differentiated. The data presented suggest that the b alpha 1 gene encodes a prominent neuronal and glial alpha-tubulin and that b alpha 1 expression is a function of the differentiated state of these cells.

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Selected References

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  • Kirschner MW. Microtubule assembly and nucleation. Int Rev Cytol. 1978;54:1–71. [PubMed]
  • Cleveland DW, Lopata MA, MacDonald RJ, Cowan NJ, Rutter WJ, Kirschner MW. Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes. Cell. 1980 May;20(1):95–105. [PubMed]
  • Wilde CD, Crowther CE, Cripe TP, Gwo-Shu Lee M, Cowan NJ. Evidence that a human beta-tubulin pseudogene is derived from its corresponding mRNA. Nature. 1982 May 6;297(5861):83–84. [PubMed]
  • Lee MG, Lewis SA, Wilde CD, Cowan NJ. Evolutionary history of a multigene family: an expressed human beta-tubulin gene and three processed pseudogenes. Cell. 1983 Jun;33(2):477–487. [PubMed]
  • Lee MG, Loomis C, Cowan NJ. Sequence of an expressed human beta-tubulin gene containing ten Alu family members. Nucleic Acids Res. 1984 Jul 25;12(14):5823–5836. [PMC free article] [PubMed]
  • Ben-Ze'ev A, Farmer SR, Penman S. Mechanisms of regulating tubulin synthesis in cultured mammalian cells. Cell. 1979 Jun;17(2):319–325. [PubMed]
  • Cleveland DW, Lopata MA, Sherline P, Kirschner MW. Unpolymerized tubulin modulates the level of tubulin mRNAs. Cell. 1981 Aug;25(2):537–546. [PubMed]
  • Lopata MA, Havercroft JC, Chow LT, Cleveland DW. Four unique genes required for beta tubulin expression in vertebrates. Cell. 1983 Mar;32(3):713–724. [PubMed]
  • Kemphues KJ, Raff RA, Kaufman TC, Raff EC. Mutation in a structural gene for a beta-tubulin specific to testis in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3991–3995. [PubMed]
  • Kemphues KJ, Raff EC, Raff RA, Kaufman TC. Mutation in a testis-specific beta-tubulin in Drosophila: analysis of its effects on meiosis and map location of the gene. Cell. 1980 Sep;21(2):445–451. [PubMed]
  • Distel RJ, Kleene KC, Hecht NB. Haploid expression of a mouse testis alpha-tubulin gene. Science. 1984 Apr 6;224(4644):68–70. [PubMed]
  • Lemischka I, Sharp PA. The sequences of an expressed rat alpha-tubulin gene and a pseudogene with an inserted repetitive element. Nature. 1982 Nov 25;300(5890):330–335. [PubMed]
  • Cowan NJ, Dobner PR, Fuchs EV, Cleveland DW. Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions. Mol Cell Biol. 1983 Oct;3(10):1738–1745. [PMC free article] [PubMed]
  • Fritsch EF, Lawn RM, Maniatis T. Molecular cloning and characterization of the human beta-like globin gene cluster. Cell. 1980 Apr;19(4):959–972. [PubMed]
  • Ozaki LS, Kimura A, Shimada K, Takagi Y. ColE1 vectors for direct selection of cells carrying a hybrid plasmid. J Biochem. 1982 Apr;91(4):1155–1162. [PubMed]
  • Sanger F, Coulson AR, Barrell BG, Smith AJ, Roe BA. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980 Oct 25;143(2):161–178. [PubMed]
  • Hu N, Messing J. The making of strand-specific M13 probes. Gene. 1982 Mar;17(3):271–277. [PubMed]
  • Staden R. A new computer method for the storage and manipulation of DNA gel reading data. Nucleic Acids Res. 1980 Aug 25;8(16):3673–3694. [PMC free article] [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Ponstingl H, Krauhs E, Little M, Kempf T. Complete amino acid sequence of alpha-tubulin from porcine brain. Proc Natl Acad Sci U S A. 1981 May;78(5):2757–2761. [PubMed]
  • Valenzuela P, Quiroga M, Zaldivar J, Rutter WJ, Kirschner MW, Cleveland DW. Nucleotide and corresponding amino acid sequences encoded by alpha and beta tubulin mRNAs. Nature. 1981 Feb 19;289(5799):650–655. [PubMed]
  • Wilde CD, Chow LT, Wefald FC, Cowan NJ. Structure of two human alpha-tubulin genes. Proc Natl Acad Sci U S A. 1982 Jan;79(1):96–100. [PubMed]
  • Young RA, Hagenbüchle O, Schibler U. A single mouse alpha-amylase gene specifies two different tissue-specific mRNAs. Cell. 1981 Feb;23(2):451–458. [PubMed]
  • Shapiro SG, Schon EA, Townes TM, Lingrel JB. Sequence and linkage of the goat epsilon I and epsilon II beta-globin genes. J Mol Biol. 1983 Sep 5;169(1):31–52. [PubMed]
  • van Straaten F, Müller R, Curran T, Van Beveren C, Verma IM. Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3183–3187. [PubMed]
  • Miesfeld R, Krystal M, Arnheim N. A member of a new repeated sequence family which is conserved throughout eucaryotic evolution is found between the human delta and beta globin genes. Nucleic Acids Res. 1981 Nov 25;9(22):5931–5947. [PMC free article] [PubMed]
  • Hamada H, Kakunaga T. Potential Z-DNA forming sequences are highly dispersed in the human genome. Nature. 1982 Jul 22;298(5872):396–398. [PubMed]
  • Rogers J. Molecular biology. CACA sequences - the ends and the means? Nature. 1983 Sep 8;305(5930):101–102. [PubMed]
  • Sun L, Paulson KE, Schmid CW, Kadyk L, Leinwand L. Non-Alu family interspersed repeats in human DNA and their transcriptional activity. Nucleic Acids Res. 1984 Mar 26;12(6):2669–2690. [PMC free article] [PubMed]
  • Rich A, Nordheim A, Wang AH. The chemistry and biology of left-handed Z-DNA. Annu Rev Biochem. 1984;53:791–846. [PubMed]
  • Brunke KJ, Anthony JG, Sternberg EJ, Weeks DP. Repeated consensus sequence and pseudopromoters in the four coordinately regulated tubulin genes of Chlamydomonas reinhardi. Mol Cell Biol. 1984 Jun;4(6):1115–1124. [PMC free article] [PubMed]
  • McKnight SL, Kingsbury R. Transcriptional control signals of a eukaryotic protein-coding gene. Science. 1982 Jul 23;217(4557):316–324. [PubMed]

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