PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of bmcmicrBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Microbiology
 
BMC Microbiol. 2012; 12: 122.
Published online Jun 22, 2012. doi:  10.1186/1471-2180-12-122
PMCID: PMC3409016
Metagenome analyses of corroded concrete wastewater pipe biofilms reveal a complex microbial system
Vicente Gomez-Alvarez,1 Randy P Revetta,1 and Jorge W Santo Domingocorresponding author1
1U.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, OH, 45268, USA
corresponding authorCorresponding author.
Vicente Gomez-Alvarez: Gomez-Alvarez.Vicente/at/epa.gov; Randy P Revetta: Revetta.Randy/at/epa.gov; Jorge W Santo Domingo: Santodomingo.Jorge/at/epa.gov
Received December 19, 2011; Accepted June 22, 2012.
Abstract
Background
Concrete corrosion of wastewater collection systems is a significant cause of deterioration and premature collapse. Failure to adequately address the deteriorating infrastructure networks threatens our environment, public health, and safety. Analysis of whole-metagenome pyrosequencing data and 16S rRNA gene clone libraries was used to determine microbial composition and functional genes associated with biomass harvested from crown (top) and invert (bottom) sections of a corroded wastewater pipe.
Results
Taxonomic and functional analysis demonstrated that approximately 90% of the total diversity was associated with the phyla Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. The top (TP) and bottom pipe (BP) communities were different in composition, with some of the differences attributed to the abundance of sulfide-oxidizing and sulfate-reducing bacteria. Additionally, human fecal bacteria were more abundant in the BP communities. Among the functional categories, proteins involved in sulfur and nitrogen metabolism showed the most significant differences between biofilms. There was also an enrichment of genes associated with heavy metal resistance, virulence (protein secretion systems) and stress response in the TP biofilm, while a higher number of genes related to motility and chemotaxis were identified in the BP biofilm. Both biofilms contain a high number of genes associated with resistance to antibiotics and toxic compounds subsystems.
Conclusions
The function potential of wastewater biofilms was highly diverse with level of COG diversity similar to that described for soil. On the basis of the metagenomic data, some factors that may contribute to niche differentiation were pH, aerobic conditions and availability of substrate, such as nitrogen and sulfur. The results from this study will help us better understand the genetic network and functional capability of microbial members of wastewater concrete biofilms.
Articles from BMC Microbiology are provided here courtesy of
BioMed Central