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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Cell Rep. Author manuscript; available in PMC 2012 July 31.
Published in final edited form as:
PMCID: PMC3408582
NIHMSID: NIHMS379915

Selective activation of cholinergic interneurons enhances accumbal phasic dopamine release: setting the tone for reward processing

Abstract

Dopamine (DA) plays a critical role in motor control, addiction and reward-seeking behaviors, and its release dynamics have traditionally been linked to changes in midbrain dopamine neuron activity. Here, we report that selective endogenous cholinergic activation achieved via in vitro optogenetic stimulation of nucleus accumbens (NAc), a terminal field of dopaminergic neurons, elicits real-time DA release. This mechanism occurs via direct actions on DA terminals, does not require changes in neuron firing within the midbrain and is dependent on glutamatergic receptor activity. More importantly, we demonstrate that in vivo selective activation of cholinergic interneurons (CINs) is sufficient to elicit DA release in the NAc. Therefore, the control of accumbal extracellular DA levels by endogenous cholinergic activity results from a complex convergence of neurotransmitter/neuromodulator systems that may ultimately synergize to drive motivated behavior.

Introduction

The mesolimbic DA system, comprised mainly of the rostral dopaminergic projection from the ventral tegmental area (VTA) to the NAc, (Wise, 2004; Sulzer, 2011), is crucial for decision making, motivated behaviors and addiction. Patterns and levels of DA in NAc are traditionally determined by the combination of firing of VTA neurons and the dynamics of DA release from the axon terminals.

Although cholinergic interneurons (CINs) are 2-5% of all striatal neurons, they establish an extensive arrangement of axons and form a diffuse neurotransmission system (Descarries et al., 1997; Descarries and Mechawar, 2000). Therefore, cholinergic activity in the striatum has long been hypothesized to play a role in the modulation of DA release (Giorguieff et al., 1976; Zhou et al., 2001; Zhang and Sulzer, 2004; Threlfell et al., 2010). Genetic deletion or pharmacological manipulation studies of nicotinic cholinergic (nAChRs) as well as muscarinic (mAChRs) receptors, have shown that they modulate electrically-evoked DA release in the striatum (Exley et al., 2012; Zhou et al., 2001; Exley et al., 2008, 2011; Zhang et al., 2009; Threlfell et al., 2010). Moreover, nAChR-targeted drugs differentially alter DA release in a frequency-dependent manner. This finding has led to the notion that a “high-pass” filter dependent on antagonism of nAChRs or nicotine, facilitates burst release of DA (Exley and Cragg, 2008), an activity pattern observed mainly following the presentation of reward and reward-predicting cues (Mirenowicz and Schultz, 1996; Nakahara et al., 2004; Roesch et al., 2007; Schultz, 2007; Apicella et al., 1991).

However, the precise role that endogenous cholinergic activity exerts on DA release in the NAc has not been explored. Endogenous release of acetylcholine (ACh) obtained by optogenetic control of CINs allows for the elucidation of cholinergic receptor action when activation occurs by the natural ligand. To examine the effect of endogenously released ACh on terminal DA release we used a combination of optogenetic techniques for selective stimulation of CINs, combined with electrophysiology, real-time detection of DA levels and pharmacology. Activation of CINs is sufficient to evoke DA release in the NAc, independently of contingent activation of the VTA, in vitro and in vivo. We further reveal that endogenous cholinergic control of DA release is not only mediated by nAChRs and modulated by mAChRs, but also results from actions at AMPA receptors. Hence, our results show that endogenous cholinergic activity exerts a powerful influence on accumbal DA release and that this phenomenon recruits a previously unrecognized convergence of neurotransmitter systems.

Results

Endogenous cholinergic activity elicits terminal dopamine release

Studies of nAChR dynamics (desensitization or antagonism) have long suggested that endogenous cholinergic tone may establish the baseline for the probability of DA release modulates DA release in the striatum (Giorguieff et al., 1976; Zhou et al., 2001; Zhang and Sulzer, 2004; Threlfell et al., 2010). This phenomenon has a presynaptic locus of action, as studied by application of exogenous acetylcholine (ACh) or ligands of the nAChR in striatal synaptosomes (Rapier et al., 1990; Wonnacott et al., 2000; Chéramy et al., 1996) or slices (Giorguieff et al., 1977; Wonnacott et al., 2000). However, is not known whether selective activation of CINs and subsequent release of endogenous ACh can directly control DA release. To test this hypothesis, we utilized optogenetic techniques to selectively activate CINs in the NAc. Briefly, we injected an adeno-associated virus encoding channelrhodopsin2 (ChR2) and enhanced yellow fluorescent protein (eYFP) into the NAc of mice expressing Cre-recombinase downstream of the choline-acetyltransferase (ChAT) promoter (ChAT-Cre mice) (see Methods). Four weeks after viral injection, studies were performed in coronal slices of the NAc.

First, we tested the functionality of CINs in our in vitro preparation by performing whole-cell recordings. Under the current-clamp configuration, YFP-positive neurons (putative CINs) displayed the characteristic spontaneous, tonic firing at a rate of ~10 Hz [the average of the membrane potential value cycles between action potentials was -28 mV (Fig. 1A)]. Under voltage clamp, delivery of a single optical (100 ms) or a brief (4 ms) pulse, elicited an inward current that lasted for the length of the pulse (n = 6, Fig. 1A). Furthermore, delivery of a 4 msec blue light pulse under current clamp conditions induced a single action potential on YFP-positive cells (Fig. 1A). Mean latency between the start of the light pulse and the start of the action potential was 5.0 ± 0.36 msec (n = 5, confirming that optical excitation of ChR2 reliably drives generation of action potentials on accumbal CINs). Additionally, histological analyses confirmed expression of ChR2-eYFP (Fig. 1B).

Figure 1
Selective optical stimulation of ChR2-expressing CINs elicits accumbal dopamine release

To verify if selective stimulation of CINs is sufficient to evoke accumbal DA release, optical stimulation was delivered by a 125 μm optical fiber placed on the surface of the slice, while real-time changes in DA release were measured with fast-scan cyclic voltammetry (FSCV) using a glass-encased carbon fiber placed 100 μm away from the optical fiber (Fig. 1C). Single pulse optical stimulation (4 ms duration square, 10 mW) in coronal slices of NAc from ChR2-expressing ChAT-Cre mice elicited an immediate and robust increase in DA levels, which was comparable to single pulse electrical stimulation (400 mA; 4 ms duration square; 23 paired measurements made in 12 animals) (Figure 1D-G). This result confirms that endogenous ACh actions on DA terminals are sufficient to elicit DA release in the NAc.

Frequency-dependence of cholinergic control of dopamine release

Because of cholinergic receptor agonists modulate the release of DA, we hypothesized that changes in CIN firing rate should correspondingly enhance DA release. To test this, we measured DA following application of different patterns of CIN optical stimulation. First, we monitored DA levels in response to paired pulse optical stimulations at different intervals. When two pulses of blue light (10 mW, 4 msec duration) were delivered at an interval of 2 sec, the second pulse triggered a second peak of DA, which was 8.3 ± 1.6 % (n=4), of the first peak (Fig 2A). Subsequent tests showed lessened reduction in the second peak down of 16 ± 2.1% at 5 s intervals (n=4), 31.5 ± 3.1% at 10 s (n=4), 66.0 ± 3.0% at 30 s (n=4) and 84.75 ± 1.1% at 60 sec interval (n=4) (Fig. 2A,B). This suggests that when the conditions for endogenous ACh release are met, a frequency-dependent limiting factor for ACh/DA release exists, most probably resulting from activation of DA or/and ACh auto-receptors. Next, we monitored DA levels following different patterns of optical stimulation to determine the optimal range of CIN activity resulting in enhanced DA release. When compared to single pulse stimulation (n=17), analysis of T80 values (see supplemental materials) of DA concentration versus time curves showed a significant T80 increase following stimulation at 5Hz (p < 0.0001, n= 15) and at 10Hz (p < 0.0001, n=16) but not at 30 Hz (n=10) (Fig. 2C, top). DA peak values were also significantly different when triggered by 5Hz (p < 0.05) and 10Hz (p < 0.001), when compared to single pulse stimulation (Fig. 2C, bottom) This suggests that CIN firing rates between ~5-10 Hz (frequencies well within the normal range of CIN firing) preferentially control release mechanisms. Application of TTX completely abolished DA release triggered by optical activation of CINs with a single pulse or 10 Hz trains (n=2; data not shown), confirming that the control of CIN activity over DA release is action potential-dependent.

Figure 2
Frequency-dependent response of DA release evoked by stimulation of CINs

Application of nicotine acts as a “high-pass” filter facilitating burst release of DA (Exley and Cragg, 2008). Specifically, electrical stimulation frequencies ≤10 Hz elicit lower DA levels compared to control, while stimulation frequencies ≥25 Hz elicit higher DA levels compared to control (Rice and Cragg, 2004). We tested if this filtering phenomenon occurs following sustained CIN activation. To do this, we applied brief electrical stimulation at different frequencies, while long optical stimulation trains to CINs (50 pulses of 4 ms duration delivered at 5 Hz, 10 mW) were applied. Under these conditions, all of the tested protocols of electrical stimulation (1 pulse, 6 pulses at 30Hz and 6 pulses of 100Hz) evoked lower peak levels of DA release, compared to peak levels obtained in absence of optical stimulation (17.6 % at 1 pulse, p < 0.0001; 51.3% at 30Hz, p < 0.0001, 74.1% at 100Hz, p > 0.05; n= 3-6) (Fig. 2D). Therefore, endogenous cholinergic activity under the present experimental conditions does not produce the high-pass filtering elicited by bath application of nicotine. To confirm whether CINs follow high frequency patterns of optical stimulation, we performed intracellular electrophysiological recordings. Under whole-cell current clamp, optical stimulation of CINs at 10 Hz elicited a corresponding sequence of action potentials at 10 Hz (Fig. 2E). However, optical stimulation at 30 Hz elicited a sequence of action potentials where only the first action potential reached full amplitude (Fig. 2F), suggesting that CINs optimally follow optical stimulation at a rate of ~10 Hz. However, CIN responsiveness is limited at higher stimulation frequencies (although this may also result from ChR2 channel dynamics).

β2-containing nicotinic receptors mediate DA release evoked by endogenous cholinergic activity

nAChRs are involved in the presynaptic control of DA release in the striatum (Hersch et al., 1994; Marshall et al., 2002; Rapier et al., 1990; Wonnacott et al., 2000). To confirm that CIN-driven enhancement of DA release is mediated by activation of nAChRs, we monitored DA levels while performing selective optical activation of CINs in the absence and presence of nAChR antagonists. Mecamylamine (n=6) decreased CIN stimulation-induced DA release with a half maximal inhibitory concentration (IC50) of 0.61 μM (Fig. 3A). The highest dose used (20 μM) decreased DA levels by 85.8 ± 3 % compared to pre-drug conditions.

Figure 3
Modulation of DA levels evoked by endogenous cholinergic activity

In mammals, combinations of nAChR subunits from subfamilies II (α7) and III (α2–α6, β2–β4) result in the formation of functional hetero- and homo- pentamers (Le Novère et al., 2002). In striatal DA terminals, heteropentamers display two α/β pairs in the form of α4/β2 and/ or α6/β2 and/or α4/β4 (Champtiaux et al., 2003). Nicotinic control of striatal DA release depends on β2 subunit-containing nAChRs (Zhou et al., 2001; Exley et al., 2012). To verify the role of β2-containing receptors in the augmentation of accumbal DA release by selective activation of CINs, we examined the effect of the β2-containing nAChR antagonist dihydro-β-erythroidine (DHβE). Application of DHβE (1 μM) resulted in a potent reduction of DA peak levels elicited by optical stimulation (by 89.05 % relative to pretreatment values; p < 0.0001; n=4) (Fig. 3B).

Glutamate acting at AMPA receptors mediates DA release evoked by selective activation of CINs

Glutamate receptors also control DA release presynaptically (Desce et al., 1992; Krebs et al., 1991; Chéramy et al., 1986a, 1998, 1996) and CINs have recently been shown to release glutamate (Higley et al., 2011; Guzman et al., 2011). Moreover, several effects evoked by CIN activity are thought to be mediated by glutamate and not by ACh (Guzman et al., 2011). Because of our findings that endogenous cholinergic activity drives DA release in a nAChR-dependent fashion, we next examined if this occurred, at least in part, through activation of AMPA receptors. First, we confirmed that stimulation of CINs led to glutamate release. Under whole-cell voltage-clamp, single pulse optical stimulation of CINs evoked a single EPSC in medium spiny neurons (MSNs) (Fig. 3C). Furthermore, optical train stimulation failed to produce subsequent EPSCs following the initial pulse in the trains (5 pulses at 2 or 30 Hz; see supplemental material). Bath application of the AMPA receptor antagonist NBQX (5 μM) attenuated the EPSC amplitude to 18.6% ± 3.4% of the pretreatment value; p = 0.0002, n = 4. This confirms that selective optical stimulation of CINs drives AMPA receptor activation on MSNs (Higley et al., 2011). Next, we monitored DA release changes triggered by selective CIN optical stimulation following NBQX. When added to the bath solution, NBQX (5 μM) significantly decreased the peak amplitude of DA release by 40.3% p < 0.01, n = 7; Fig. 3D, confirming that AMPA receptors contribute to the control of DA release evoked by CIN activation in the NAc.

mAChR receptor activation modulates cholinergic-driven release of dopamine

mAChRs are also present in the striatum (Hersch et al., 1994) and the mAChR agonist oxotremorine decreases DA release evoked by single pulse electrical stimulation, but increases release evoked by high frequencies (Threlfell et al., 2010). Furthermore, genetic deletion of the muscarinic M4 subunit prevents this modulation of DA release in NAc (Threlfell et al., 2010). However, in contrast to nAChRs, there is no evidence of M4 expression on DA terminals, suggesting that mAChR activation preferentially inhibits ACh release in the NAc (Threlfell et al., 2010; Threlfell and Cragg, 2011).

To identify the role of mAChRs on DA release evoked by endogenous cholinergic activity, we next tested the effect of the mAChR antagonist scopolamine on DA levels triggered by selective stimulation of CINs. When scopolamine (1 μM) was applied to the bath, DA peak levels evoked by a single pulse of selective CIN stimulation only modestly increased to 112 % of pretreatment values; p = 0.38. When CINs were optically stimulated by a train of 30 pulses at 10 or 5 Hz (10 mW, 4 msec duration per pulse), peak DA levels significantly increased to 146-148 %, p < 0.05) of pretreatment values (n=6-12) (Fig. 3E,F) suggesting that blocking mAChRs relieves the inhibition of ACh release and that this effect is preferentially seen when trains of action potentials drive mobilization of the ACh releasable pool.

In vivo selective stimulation of accumbal cholinergic interneurons induces dopamine release

The present results suggest that termino-terminal endogenous cholinergic activity controls DA release and that this involves activation of glutamate receptors in vitro. However, it is not known if promoting DA release by selective activation of CINs occurs in vivo. To test this possibility, FSCV recordings combined with optical stimulation from adjacent sites (200 μm separation) were performed by implantation of an optical fiber/carbon fiber arrangement (optrode, see Methods, Fig. 4A) into the NAc of urethane-anesthetized mice. Because under these recording conditions the recording electrode cannot be optimally placed in the area of highest fluorescence under visual control, the conditions necessary to obtain CIN-evoked release were different from those used in the slice. Optical stimulation was reliably achieved by delivery of a train of blue light (473 nm wavelength, 10 mW, 4 msec duration/pulse, 150 pulses, 20 Hz) through the optical fiber of the implanted optrode. Selective activation of accumbal CINs triggered a significant increase in DA concentration (18.2 ± 2.1 nM), respect to baseline values; n= 3) (Fig. 4B,C), providing unambiguous in vivo evidence that CIN activity locally enhances DA release in the NAc.

Figure 4
In vivo selective stimulation of CINs evokes accumbal DA release

Discussion

Cholinergic receptor activation potently controls striatal levels of DA, a neuromodulator crucial for the expression of coordinated motor activity and Pavlovian cue-reward associations (reviewed by Wise, 2004; Sulzer, 2011). In this report, we characterize the effects of selective CIN activation on accumbal DA levels. We find that, while having a relatively sparse distribution, CINs profoundly modulate DAergic output in NAc.

We show that selective optogenetic stimulation of CINs evokes DA release in a β2-containing nAChR-dependent manner. While electrophysiological studies have hypothesized that dopamine can be released in a manner that is not contingent upon ongoing activity in dopaminergic fibers (Ding et al., 2010), our data reveal previously unseen dynamics of this release process directly. Furthermore, we identify the convergence of different neurotransmitter systems participating in this phenomenon. Increased DA concentration during blockade of mAChRs suggests a critical role of these receptors in controlling ACh release. Consistent with recent reports demonstrating glutamate release from CIN terminals, interfering with AMPA receptor signaling weakens optically evoked DA release. More importantly, we determine that DA release can also be evoked by blue light activation of CINs in vivo.

Study of frequency-dependent relations between CIN stimulation and DA levels showed a clear paired-pulse depression, suggesting strong mechanisms of presynaptic control of release at either, or both, CIN and DA neuron terminals. Although this has been described separately at DA and ACh synapses, more detailed studies are necessary to demonstrate how interactions between these two sites of release interact into determining final DA levels. Moreover, we report that sustained optical stimulation of CINs does not mimic the nicotine-dependent high-pass filtering of electrically evoked DA release (Exley and Cragg, 2008). Together, these results point to a crucial role of mAChR activation in limiting the effects of persistent endogenous ACh activity on nAChRs. This feedback mechanism is absent under the effect of nicotine, which promotes desensitization of nAChRs, thought to be the main mechanism underlying nicotine-evoked high-pass filtering of DA release (Rice and Cragg, 2004; Exley and Cragg, 2008). In support of this notion, we confirmed that β2-containing nAChRs mediate ACh-evoked release of DA, and that mAChRs play a predominant role in limiting endogenous ACh release, because ACh-evoked DA release is enhanced (albeit modestly) following blockade of mAChRs.

Glutamate modulates DA release by acting on dopaminergic terminals (Chéramy et al., 1986b; Krebs et al., 1991; Chéramy et al., 1998, 1996) and because CINs mediate glutamatergic transmission (Guzman et al., 2011; Higley et al., 2011), we hypothesized that a fraction of the DA released by selective stimulation of CINs involves activation of glutamate receptors. Supporting this view, we found that CIN-evoked DA release relies –at least partially– on activation of AMPA receptors. This establishes even broader implications, given that glutamate released from CINs mediates not only excitation of MSNs, as previously described (Higley et al., 2011), but also shapes accumbal DA release.

The present experiments uncover a multiplicity of regulatory mechanisms that converge to control DA release elicited by the selective activation of CINs. In behaving animals, CINs encode reward-related events (Morris et al., 2004). While DA neurons increase or decrease their basal firing rate in response to the presentation or omission of reward, CINs respond with a brief pause independently of the outcome (Aosaki et al., 1994; Morris et al., 2004). This has been interpreted as the establishment of the appropriate temporal window for contingencies to be encoded, while DAergic responses are theorized to carry a learning signal about future outcomes (Morris et al., 2004). Here, we determined that in vivo DA release is in fact triggered by endogenous release of ACh. This allows new considerations to be taken into account for the way that CIN activity may set the stage for DA neuron activity to produce its postsynaptic effects. Reward-related activity of CINs consists of several phases (initial rise, pause, and second rise) (Morris et al., 2004; Aosaki et al., 1994; Shimo and Hikosaka, 2001; Apicella et al., 1991, 2011; Apicella, 2007). In response to reward, the peak of the initial phase coincides with the rise in DA neuron activity. We speculate that the initial rise phase of CIN firing rate and subsequent ACh-Glu release could act as a priming event, exciting MSN neurons and boosting DA release originating from the midbrain, whereas the transition to the pause in CIN activity may allow for the hypothesized contrast enhancement of the midbrain signal (Zhang and Sulzer, 2004; Cragg, 2006; Nicola et al., 2004). Moreover, activation of nAChRs promotes long-term depression of corticostriatal glutamatergic transmission via regulation of DA release (Partridge et al., 2002), and thus our findings provide evidence of a link between CIN activity and synaptic plasticity implicated in reinforcement learning. Our results generate a novel conceptual framework with which to interpret the regulation of accumbal DA release and its role in reward-directed behaviors.

Methods

Subjects

Male ChAT-Cre mice were used. They were single-housed in a room under 12 hour light/dark cycle and food/water was available ad libitum.

Stereotaxic virus injection

An AAV-ChR2 vector was injected bilaterally (500 nl/side) into the nucleus accumbens of mice that were allowed to recover for 4 - 8 weeks after virus injection, before any subsequent intervention.

Histology

At least 4 weeks after viral injection mice were perfused transcardially with 4% paraformaldehyde. Brains were extracted, sliced and processed with an anti-ChAT polyclonal antibody and a fluorescein-conjugated secondary antibody. Sections were then mounted and visualized by epifluorescence with a Leica DM LB microscope to identify ChAT-labeled and eYFP-positive neurons.

In vitro optical stimulation and fast-scan cyclic voltammetry

4 weeks after virus injection, mice were killed by decapitation, the brain quickly removed and incubated in modified Krebs buffer. Coronal slices containing the NAc (250 μm thick) were obtained, incubated and then transferred to the recording chamber, perfused at 1 ml/min with 34°C oxygenated Krebs buffer.

Using a cylindrical carbon fiber, voltammetric recordings (versus an Ag/AgCl reference electrode) were performed using Demon Voltammetry and Analysis Software. Optical and electrical stimulation were delivered through an optical fiber in apposition with the brain slice and through a bipolar tungsten electrode in contact with the slice, respectively.

Whole-cell electrophysiological recordings

After incubation, brain striatal slices were transferred to the recording chamber, superfused with artificial cerebrospinal fluid and maintained at 30 ± 1° C. Current- and voltage- clamp recordings of CINs were performed in unmodified aCSF, while voltage clamp recordings of MSN EPSCs were performed in aCSF containing 50 μM picrotoxin to block GABAA-mediated currents. Optical stimulation was delivered via the epifluorescence light path. Clampex 10.3 software was used for data acquisition.

In vivo optical stimulation and fast-scan cyclic voltammetry

4 weeks after virus injection, access to the NAc was obtained under stereotaxic surgery. An optrode (carbon fiber/optical fiber) was implanted and voltammetric recordings (versus an Ag/AgCl reference electrode) performed using Tar Heel CV software.

Statistical Analysis

Unless otherwise indicated, 2-way ANOVA followed by Bonferroni corrections was performed using Prism (GraphPad Software, CA).

Highlights

  • Selective cholinergic interneuron activation evokes accumbal dopamine release.
  • Cholinergic control of DA involves glutamatergic actions in vitro.
  • Accumbal regulation of DA release by cholinergic activity also occurs in vivo.
  • This interaction may be a novel target for the treatment of disorders of motivation.

Supplementary Material

01

Acknowledgements

This work was supported by NIDA grants DA022340 and DA025890 to JFC, and the Intramural Research Program at NIAAA (YM, BNM, DML) or NIDA (MM and HLW). The authors would like to acknowledge Dr. Garret Stuber for assistance with optogenetic techniques.

Footnotes

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