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Autoimmune Dis. 2012; 2012: 265823.
Published online Jul 19, 2012. doi:  10.1155/2012/265823
PMCID: PMC3407603
Identification of TNIP1 Polymorphisms by High Resolution Melting Analysis with Unlabelled Probe: Association with Systemic Lupus Erythematosus
Jie Zhang, 1 , 2 , 3 Yuewen Chen, 1 , 3 , 4 Yong Shao, 1 , 2 , 3 Qi Wu, 1 , 3 Ming Guan, 5 Wei Zhang, 1 , 3 Jun Wan, 3 , 6 * and Bo Yu 2 *
1Shenzhen Key Lab for Translational Medicine of Dermatology, Shenzhen PKU-HKUST Medical Center, No. 1120 Lian-Hua Road, Futian District, Shenzhen, Guangdong 518036, China
2Department of Dermatology, Shenzhen Hospital, Peking University, Shenzhen, Guangdong 518036, China
3Biomedical Research Institute, Shenzhen PKU-HKUST Medical Center, No. 1120 Lianhua Road, Futian District, Shenzhen, Guangdong 518036, China
4JNU-HKUST Joint Laboratory, Jinan University, Guangdong 510632, China
5Department of Clinical Laboratory, Shanghai Worldwide Medical Center, Huashan 200040, China
6Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong
*Jun Wan: wanj/at/ust.hk and
*Bo Yu: yubomd/at/hotmail.com
Academic Editor: Hiroshi Okamoto
Received March 6, 2012; Accepted May 31, 2012.
Abstract
Background. TNFα-induced protein 3 (TNFAIP3) interacting with protein 1 (TNIP1) acts as a negative regulator of NF-κB and plays an important role in maintaining the homeostasis of immune system. A recent genome-wide association study (GWAS) showed that the polymorphism of TNIP1 was associated with the disease risk of SLE in Caucasian. In this study, we investigated whether the association of TNIP1 with SLE was replicated in Chinese population. Methods. The association of TNIP1 SNP rs7708392 (G/C) was determined by high resolution melting (HRM) analysis with unlabeled probe in 285 SLE patients and 336 healthy controls. Results. A new SNP rs79937737 located on 5 bp upstream of rs7708392 was discovered during the HRM analysis. No association of rs7708392 or rs79937737 with the disease risk of SLE was found. Furthermore, rs7708392 and rs79937737 were in weak linkage disequilibrium (LD). Hypotypes analysis of the two SNPs also showed no association with SLE in Chinese population. Conclusions. High resolution melting analysis with unlabeled probes proves to be a powerful and efficient genotyping method for identifying and screening SNPs. No association of rs7708392 or rs79937737 with the disease risk of SLE was observed in Chinese population.
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